Product Info Summary
SKU: | A00223 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Transcription activator BRG1 SMARCA4 Antibody
SKU/Catalog Number
A00223
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transcription activator BRG1 SMARCA4 Antibody (Catalog # A00223). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
SMARCA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Transcription activator BRG1 SMARCA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00223)
Host
Rabbit
Contents
SMARCA4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
SMARCA4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human SMARCA4. The immunogen is located within amino acids 1420 - 1470 of SMARCA4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
SMARCA4 antibody is human, mouse and rat reactive. Multiple isoforms of SMARCA4 are known to exist.
Reactive Species
A00223 is reactive to SMARCA4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
184646 MW
Background of Brg1
The SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 4 (SMARCA4) protein, also known as BRG1, is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila (1). Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes (2). The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin (3). In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44 (4,5).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00223 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
SMARCA4 antibody can be used for detection of SMARCA4 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 2 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of SMARCA4 in SK-N-SH cell lysate with SMARCA4 antibody at 1 μg/ml.
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Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.
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Immunohistochemistry of SMARCA4 in rat brain tissue with SMARCA4 antibody at 5 μg/mL.
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Immunohistochemistry of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 2 μg/ml.
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Immunofluorescence of SMARCA4 in mouse testis tissue with SMARCA4 antibody at 20 μg/ml.
Green: SMARCA4 Antibody (A00223)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For SMARCA4 (Source: Uniprot.org, NCBI)
Gene Name
SMARCA4
Full Name
Transcription activator BRG1
Weight
184646 MW
Superfamily
SNF2/RAD54 helicase family
Alternative Names
BRG1, SNF2, SWI2, MRD16, RTPS2, BAF190, SNF2L4, SNF2LB, hSNF2b, BAF190A, BRG1, SNF2B, Transcription activator BRG1, ATP-dependent helicase SMARCA4 SMARCA4 BAF190, BAF190A, BRG1, CSS4, MRD16, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 transcription activator BRG1|ATP-dependent helicase SMARCA4|BRG1-associated factor 190A|BRM/SWI2-related gene 1|SNF2-like 4|brahma protein-like 1|global transcription activator homologous sequence|homeotic gene regulator|mitotic growth and transcription activator|nuclear protein GRB1|protein BRG-1|protein brahma homolog 1|sucrose nonfermenting-like 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SMARCA4, check out the SMARCA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SMARCA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transcription activator BRG1 SMARCA4 Antibody (A00223)
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