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Product Info Summary
| SKU: | M00730 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bid Rabbit Monoclonal Antibody
SKU/Catalog Number
M00730
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Bid Rabbit Monoclonal Antibody catalog # M00730. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bid Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00730)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAGC-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bid
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00730 is reactive to BID in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
90 kDa
Calculated molecular weight
21995 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00730 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:10
FC 1:50
Positive Control
WB: Jurkat cell
IHC: human lung tissue
ICC/IF: Jurkat cell
Validation Images & Assay Conditions
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Western blot analysis of Bid expression in Jurkat cell lysate.
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Immunohistochemical analysis of paraffin-embedded human lung, using Bid Antibody.
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Immunofluorescent analysis of Jurkat cells, using Bid Antibody .
Protein Target Info & Infographic
Gene/Protein Information For BID (Source: Uniprot.org, NCBI)
Gene Name
BID
Full Name
BH3-interacting domain death agonist
Weight
21995 MW
Alternative Names
BH3-interacting domain death agonist;p22 BID;BID;BH3-interacting domain death agonist p15;p15 BID;BH3-interacting domain death agonist p13;p13 BID;BH3-interacting domain death agonist p11;p11 BID;BID; BID FP497 BH3 interacting domain death agonist BH3-interacting domain death agonist|Human BID coding sequence|apoptic death agonist|desmocollin type 4|p22 BID
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BID, check out the BID Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BID: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bid Rabbit Monoclonal Antibody (M00730)
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No publications found for M00730
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Customer Q&As
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16 Customer Q&As for Anti-Bid Rabbit Monoclonal Antibody
Question
We are currently using anti-Bid Rabbit Monoclonal antibody M00730 for human tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
The anti-Bid Rabbit Monoclonal antibody (M00730) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-28
Question
I see that the anti-Bid Rabbit Monoclonal antibody M00730 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-19
Question
Our lab were satisfied with the WB result of your anti-Bid Rabbit Monoclonal antibody. However we have been able to see positive staining in embryonic kidney isoform 2: mitochondrion membrane using this antibody. Is that expected? Could you tell me where is BID supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-25
Answer
From literature, embryonic kidney does express BID. Generally BID expresses in cytoplasm., bh3-interacting domain death agonist p15:, bh3-interacting domain death agonist p13:, isoform 1: cytoplasm, isoform 3: cytoplasm, isoform 2: mitochondrion membrane. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2019-10-25
Question
My question regarding product M00730, anti-Bid Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00730 anti-Bid Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-09
Question
Does anti-Bid Rabbit Monoclonal antibody M00730 work for IP with blood?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
According to the expression profile of blood, BID is highly expressed in blood. So, it is likely that anti-Bid Rabbit Monoclonal antibody M00730 will work for IP with blood.
Boster Scientific Support
Answered: 2019-07-04
Question
We have been able to see staining in human brain skin. What should we do? Is anti-Bid Rabbit Monoclonal antibody supposed to stain brain skin positively?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
From what I have seen in literature brain skin does express BID. From what I have seen in Uniprot.org, BID is expressed in blood, brain skin, embryonic kidney, liver, among other tissues. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2018-12-25
Question
Does M00730 anti-Bid Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-07-16
Answer
As indicated on the product datasheet, M00730 anti-Bid Rabbit Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-07-16
Question
Is this M00730 anti-Bid Rabbit Monoclonal antibody reactive to the isotypes of BID?
F. Thomas
Verified customer
Asked: 2018-05-23
Answer
The immunogen of M00730 anti-Bid Rabbit Monoclonal antibody is A synthesized peptide derived from human Bid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-23
Question
We bought anti-Bid Rabbit Monoclonal antibody for IF on brain skin in a previous project. I am using human, and We intend to use the antibody for IP next. I would like examining brain skin as well as liver in our next experiment. Could give a recommendation on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
I looked at the website and datasheets of our anti-Bid Rabbit Monoclonal antibody and it seems that M00730 has been validated on human in both IF and IP. Thus M00730 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-03-06
Question
We need using your anti-Bid Rabbit Monoclonal antibody for extrinsic apoptotic signaling pathway via death domain receptors studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-03-02
Answer
We appreciate your inquiry. This M00730 anti-Bid Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for IP, IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-03-02
Question
I was wanting to use your anti-Bid Rabbit Monoclonal antibody for IP for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2017-12-26
Answer
You can see on the product datasheet, M00730 anti-Bid Rabbit Monoclonal antibody has been validated for IP, IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-12-26
Question
I was wanting to use to test anti-Bid Rabbit Monoclonal antibody M00730 on human blood for research purposes, then I may be interested in using anti-Bid Rabbit Monoclonal antibody M00730 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
F. Krishna
Verified customer
Asked: 2017-11-08
Answer
The products we sell, including anti-Bid Rabbit Monoclonal antibody M00730, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-08
Question
See attached the WB image, lot number and protocol we used for blood using anti-Bid Rabbit Monoclonal antibody M00730. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-12
Question
Is a blocking peptide available for product anti-Bid Rabbit Monoclonal antibody (M00730)?
Verified Customer
Verified customer
Asked: 2017-06-06
Answer
We do provide the blocking peptide for product anti-Bid Rabbit Monoclonal antibody (M00730). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-06-06
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Bid Rabbit Monoclonal antibody M00730. Let me know if you need anything else.
E. Anderson
Verified customer
Asked: 2014-03-06
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-06
Question
Do you have a BSA free version of anti-Bid Rabbit Monoclonal antibody M00730 available?
A. Moore
Verified customer
Asked: 2013-09-30
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Bid Rabbit Monoclonal antibody M00730 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-09-30
