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Product Info Summary
| SKU: | A00730-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Bid DyLight® 488 conjugated Antibody
SKU/Catalog Number
A00730-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Bid DyLight® 488 conjugated Antibody catalog # A00730-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Bid DyLight® 488 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00730-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequence identity with mouse and rat Bid, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00730-Dyl488 is reactive to BID in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
21.995kDa
Background of BID
BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00730-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: K562 cell
Validation Images & Assay Conditions
Click image to see more details
1. Flow Cytometry analysis of K562 cells using anti-Human Bid antibody (A00730-Dyl488).
Overlay histogram showing K562 cells stained with A00730-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bid Antibody (A00730-Dyl488,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For BID (Source: Uniprot.org, NCBI)
Gene Name
BID
Full Name
BH3-interacting domain death agonist
Weight
21.995kDa
Alternative Names
BH3-interacting domain death agonist; p22 BID; BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID; BID BID FP497 BH3 interacting domain death agonist BH3-interacting domain death agonist|Human BID coding sequence|apoptic death agonist|desmocollin type 4|p22 BID
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BID, check out the BID Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BID: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Bid DyLight® 488 conjugated Antibody (A00730-Dyl488)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Human Bid DyLight® 488 conjugated Antibody
Question
Do you have a BSA free version of anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488 available?
R. Bhatt
Verified customer
Asked: 2020-05-08
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-05-08
Question
I see that the anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-02
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-02
Question
I was wanting to use your anti-Human Bid DyLight® 488 conjugated antibody for Flow Cytometry for human brain skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain skin identification?
Verified Customer
Verified customer
Asked: 2019-04-15
Answer
You can see on the product datasheet, A00730-Dyl488 anti-Human Bid DyLight® 488 conjugated antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain skin in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-15
Question
Is a blocking peptide available for product anti-Human Bid DyLight® 488 conjugated antibody (A00730-Dyl488)?
Verified Customer
Verified customer
Asked: 2018-10-11
Answer
We do provide the blocking peptide for product anti-Human Bid DyLight® 488 conjugated antibody (A00730-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-10-11
Question
See below the WB image, lot number and protocol we used for brain skin using anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488. Please let me know if you require anything else.
G. Thomas
Verified customer
Asked: 2018-10-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-11
Question
Is this A00730-Dyl488 anti-Human Bid DyLight® 488 conjugated antibody reactive to the isotypes of BID?
Verified Customer
Verified customer
Asked: 2018-05-21
Answer
The immunogen of A00730-Dyl488 anti-Human Bid DyLight® 488 conjugated antibody is E. coli-derived human Bid recombinant protein (Position: M1-D195). Human Bid shares 64% and 61% amino acid (aa) sequence identity with mouse and rat Bid, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-05-21
Question
We have observed staining in human liver. Any tips? Is anti-Human Bid DyLight® 488 conjugated antibody supposed to stain liver positively?
S. Rodriguez
Verified customer
Asked: 2017-12-13
Answer
From literature liver does express BID. From Uniprot.org, BID is expressed in blood, brain skin, embryonic kidney, liver, among other tissues. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2017-12-13
Question
Our lab want to know about using your anti-Human Bid DyLight® 488 conjugated antibody for hepatocyte apoptotic process studies. Has this antibody been tested with western blotting on k562 cells? We would like to see some validation images before ordering.
H. Parker
Verified customer
Asked: 2017-10-11
Answer
I appreciate your inquiry. This A00730-Dyl488 anti-Human Bid DyLight® 488 conjugated antibody is validated on k562 cells. It is guaranteed to work for Flow Cytometry in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-11
Question
Can you help my question with product A00730-Dyl488, anti-Human Bid DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00730-Dyl488 anti-Human Bid DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain skin using anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-22
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-22
Question
Will anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488 work for Flow Cytometry with brain skin?
J. Miller
Verified customer
Asked: 2016-11-24
Answer
According to the expression profile of brain skin, BID is highly expressed in brain skin. So, it is likely that anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488 will work for Flow Cytometry with brain skin.
Boster Scientific Support
Answered: 2016-11-24
Question
My boss were satisfied with the WB result of your anti-Human Bid DyLight® 488 conjugated antibody. However we have been able to see positive staining in brain skin isoform 2: mitochondrion membrane using this antibody. Is that expected? Could you tell me where is BID supposed to be expressed?
A. Johnson
Verified customer
Asked: 2016-01-21
Answer
Based on literature, brain skin does express BID. Generally BID expresses in cytoplasm., bh3-interacting domain death agonist p15:, bh3-interacting domain death agonist p13:, isoform 1: cytoplasm, isoform 3: cytoplasm, isoform 2: mitochondrion membrane. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2016-01-21
Question
Will A00730-Dyl488 anti-Human Bid DyLight® 488 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Patel
Verified customer
Asked: 2015-04-23
Answer
It shows on the product datasheet, A00730-Dyl488 anti-Human Bid DyLight® 488 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-04-23
Question
We are currently using anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
M. Mangal
Verified customer
Asked: 2015-04-06
Answer
The anti-Human Bid DyLight® 488 conjugated antibody (A00730-Dyl488) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-06
Question
We are interested in to test anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488 on human brain skin for research purposes, then I may be interested in using anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Zhang
Verified customer
Asked: 2014-03-04
Answer
The products we sell, including anti-Human Bid DyLight® 488 conjugated antibody A00730-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-03-04
