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Product Info Summary
| SKU: | A00730 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Bid Antibody Picoband®
SKU/Catalog Number
A00730
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bid Antibody Picoband® catalog # A00730. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bid Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00730)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00730 is reactive to Bid in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
21952 MW
Background of BID
BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, the human BID gene is mapped to 22q11. It is reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00730 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat, By Heat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Mouse
Positive Control
WB: mouse L929 whole cell, mouse spleen tissue, mouse RAW2647 whole cell
IHC: rat spleen tissue
FCM: Neuro-2a cell
Validation Images & Assay Conditions
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Figure 3. Flow Cytometry analysis of Neuro-2a cells using anti-Bid antibody (A00730).
Overlay histogram showing Neuro-2a cells stained with A00730 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Bid Antibody (A00730, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 1. Western blot analysis of Bid using anti-Bid antibody (A00730).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse L929 whole cell lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bid antigen affinity purified polyclonal antibody (Catalog # A00730) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bid at approximately 22 kDa. The expected band size for Bid is at 22 kDa.
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Figure 2. IHC analysis of Bid using anti-Bid antibody (A00730).
Bid was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Bid Antibody (A00730) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Bid (Source: Uniprot.org, NCBI)
Gene Name
Bid
Full Name
BH3-interacting domain death agonist
Weight
21952 MW
Alternative Names
BH3-interacting domain death agonist;p22 BID;BID;BH3-interacting domain death agonist p15;p15 BID;BH3-interacting domain death agonist p13;p13 BID;BH3-interacting domain death agonist p11;p11 BID;Bid; BID FP497 BH3 interacting domain death agonist BH3-interacting domain death agonist|Human BID coding sequence|apoptic death agonist|desmocollin type 4|p22 BID
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Bid, check out the Bid Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Bid: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bid Antibody Picoband® (A00730)
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No publications found for A00730
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Customer Q&As
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16 Customer Q&As for Anti-Bid Antibody Picoband®
Question
Is this A00730 anti-Bid antibody reactive to the isotypes of BID?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The immunogen of A00730 anti-Bid antibody is E.coli-derived mouse Bid recombinant protein (Position: M1-D195). Mouse Bid shares 64.6% and 87.2% amino acid (aa) sequence identity with human and rat Bid, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-25
Question
We have seen staining in rat embryonic kidney. Are there any suggestions? Is anti-Bid antibody supposed to stain embryonic kidney positively?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
According to literature embryonic kidney does express BID. According to Uniprot.org, BID is expressed in blood, brain skin, embryonic kidney, liver, among other tissues. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2020-03-09
Question
Please see the WB image, lot number and protocol we used for embryonic kidney using anti-Bid antibody A00730. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-27
Question
I was wanting to use your anti-Bid antibody for WB for mouse embryonic kidney on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
As indicated on the product datasheet, A00730 anti-Bid antibody has been tested for IHC, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-11
Question
I see that the anti-Bid antibody A00730 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-03
Question
Do you have a BSA free version of anti-Bid antibody A00730 available?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bid antibody A00730 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-31
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-Bid antibody A00730. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
Would anti-Bid antibody A00730 work for WB with embryonic kidney?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
According to the expression profile of embryonic kidney, BID is highly expressed in embryonic kidney. So, it is likely that anti-Bid antibody A00730 will work for WB with embryonic kidney.
Boster Scientific Support
Answered: 2019-12-12
Question
Is a blocking peptide available for product anti-Bid antibody (A00730)?
Verified Customer
Verified customer
Asked: 2019-12-06
Answer
We do provide the blocking peptide for product anti-Bid antibody (A00730). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-06
Question
We bought anti-Bid antibody for WB on brain skin in the past. I am using rat, and We intend to use the antibody for IHC next. We want examining brain skin as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
I looked at the website and datasheets of our anti-Bid antibody and I see that A00730 has been validated on rat in both WB and IHC. Thus A00730 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-12-03
Question
Can you help my question with product A00730, anti-Bid antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00730 anti-Bid antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-21
Question
We are currently using anti-Bid antibody A00730 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
The anti-Bid antibody (A00730) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-08
Question
My lab would like using your anti-Bid antibody for apoptotic mitochondrial changes studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
B. Wu
Verified customer
Asked: 2018-06-21
Answer
I appreciate your inquiry. This A00730 anti-Bid antibody is tested on mouse spleen tissue, tissue lysate, rat spleen tissue, brain tissue. It is guaranteed to work for IHC, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-06-21
Question
Would A00730 anti-Bid antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Gonzalez
Verified customer
Asked: 2018-01-23
Answer
It shows on the product datasheet, A00730 anti-Bid antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-23
Question
Our team were satisfied with the WB result of your anti-Bid antibody. However we have been able to see positive staining in embryonic kidney isoform 3: cytoplasm using this antibody. Is that expected? Could you tell me where is BID supposed to be expressed?
B. Anderson
Verified customer
Asked: 2015-06-18
Answer
Based on literature, embryonic kidney does express BID. Generally BID expresses in cytoplasm., bh3-interacting domain death agonist p15:, bh3-interacting domain death agonist p13:, isoform 1: cytoplasm, isoform 3: cytoplasm, isoform 2: mitochondrion membrane. Regarding which tissues have BID expression, here are a few articles citing expression in various tissues:
Brain, and Skin, Pubmed ID: 15489334
Embryonic kidney, Pubmed ID: 17525332
Liver, Pubmed ID: 24275569
Boster Scientific Support
Answered: 2015-06-18
Question
I am interested in to test anti-Bid antibody A00730 on mouse embryonic kidney for research purposes, then I may be interested in using anti-Bid antibody A00730 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
O. Zhang
Verified customer
Asked: 2013-03-25
Answer
The products we sell, including anti-Bid antibody A00730, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-25
