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Product Info Summary
| SKU: | EK0696 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. |
Product info
Product Name
Human CD26/DPP4 ELISA Kit PicoKine®
SKU/Catalog Number
EK0696
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD26/DPP4 ELISA Kit PicoKine® (96 Tests). Quantitate Human DPP4 in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD26/DPP4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0696)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D34-P766
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0696 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0696 is reactive to DPP4 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.
Application Guarantee
EK0696 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of DPP4
Dipeptidyl peptidase-4 (DPP4), also known as adenosine deaminase complexing protein 2 or CD26 (cluster of differentiation 26) is a protein that, in humans, is encoded by the DPP4 gene. By fluorescence in situ hybridization, the CD26 gene was mapped to 2q24.3. DPP4 plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. Furthermore, it appears to work as a suppressor in the development of cancer and tumours. CD26/ DPP4 plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0696-CAP | Anti-Human DPP4 Pre-coated 96-well strip microplate | 1 |
| EK0696-ST | Human DPP4 Standard | 2 vials, 20 ng/tube |
| EK0696-DA | Human DPP4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.008 | 0.082 | 0.124 | 0.198 | 0.366 | 0.615 | 1.164 | 1.833 |
Data Example Images
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Human CD26/DPP4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect DPP4, Dilution ratio of 1:100, concentration in serum and plasma is 3127-734 ng/ml..
Some articles we found to cite concentrations of DPP4 in samples: 25705004 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD26/DPP4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 590 | 3074 | 9263 | 536 | 3241 | 9182 |
| Standard deviation | 26.55 | 196.73 | 574.3 | 26.26 | 269 | 661.1 |
| CV (%) | 4.5% | 6.4% | 6.2% | 4.9% | 8.3% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of DPP4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 590 | 523 | 573 | 598 | 571 | 29.14 | 5.1% |
| Sample 2 | 3074 | 2972 | 3152 | 2772 | 2992 | 142.41 | 4.7% |
| Sample 3 | 9263 | 9581 | 9698 | 9711 | 9563 | 180.59 | 1.8% |
Protein Target Info & Infographic
Gene/Protein Information For DPP4 (Source: Uniprot.Org, NCBI)
Gene Name
DPP4
Full Name
Dipeptidyl peptidase 4
Weight
88279 MW
Superfamily
peptidase S9B family
Alternative Names
Dipeptidyl peptidase 4;3.4.14.5 ;ADABP;Adenosine deaminase complexing protein 2;ADCP-2;Dipeptidyl peptidase IV;DPP IV;T-cell activation antigen CD26;TP103;CD26;Dipeptidyl peptidase 4 membrane form;Dipeptidyl peptidase IV membrane form;Dipeptidyl peptidase 4 soluble form;Dipeptidyl peptidase IV soluble form;DPP4;ADCP2, CD26; DPP4 ADABP, ADCP2, CD26, DPPIV, TP103 dipeptidyl peptidase 4 dipeptidyl peptidase 4|ADCP-2|DPP IV|Gly-Pro naphthylamidase|Post-proline dipeptidyl aminopeptidase IV|T-cell activation CD26|Xaa-Pro-dipeptidylaminopeptidase|adenosine deaminase complexing protein 2|dipeptidyl peptidase IV|dipeptidylpeptidase 4|dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DPP4, check out the DPP4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DPP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD26/DPP4 ELISA Kit PicoKine® (EK0696)
Hello CJ!
EK0696 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Diagnostic and prognostic significance of serum CD26 level in Asian women with high-grade serous ovarian carcinoma
Serum CD26 levels in patients with gastric cancer: a novel potential diagnostic marker
Prognostic Significance of Serum CD26 Concentration in Patients with Esophageal Squamous Cell Carcinoma
CD26/DPP4 - a potential biomarker and target for cancer therapy
Species: Human
Serum CD26 levels in patients with gastric cancer: a novel potential diagnostic marker
Extra virgin olive oil use is associated with improved post-prandial blood glucose and LDL cholesterol in healthy subjects
KLF4 promotes hydrogen-peroxide-induced apoptosis of chronic myeloid leukemia cells involving the bcl-2/bax pathway
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Customer Q&As
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10 Customer Q&As for Human CD26/DPP4 ELISA Kit PicoKine®
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis DPP4 using the EK0696 Human DPP4 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-12-17
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2020-12-17
Question
Q: What is the optimal O.D. value for DPP4 ELISA kit? I performed your DPP4 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain DPP4 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-09-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this DPP4 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of DPP4 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-09-19
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-26
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-02-10
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-05-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Allen
Verified customer
Asked: 2018-04-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-04-30
Question
Q: can I use citrate plasma as samples in Human DPP4 Picokine® ELISA Kit (Catalog # EK0696)?
J. Miller
Verified customer
Asked: 2018-03-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of DPP4 causing degradation of its protein structure. DPP4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the DPP4 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-03-04
Question
Q: how can I thaw whole blood sample for DPP4 ELISA after freezing?
T. Robinson
Verified customer
Asked: 2018-02-10
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test DPP4 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-02-10
Question
Q: if the enzyme conjugated DPP4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DPP4 antigen?
Verified Customer
Verified customer
Asked: 2016-09-03
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2016-09-03
Question
Which form of DPP4 (membrane, soluble or total) will be detected by this ELISA kit?
Verified Customer
Verified customer
Asked: 2013-03-01
Answer
The standards in the kit are recombinant human CD26 (NSO, D34-P766), and it detects the soluble form.
Boster Scientific Support
Answered: 2013-03-01
