Product Info Summary
SKU: | M01263-5 |
---|---|
Size: | 100 μl/vial |
Reactive Species: | Goat, Human, Monkey, Mouse, Rat |
Host: | Mouse |
Application: | WB |
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Product info
Product Name
Anti-beta Actin Mouse Monoclonal Antibody
View all beta-Actin Antibodies
SKU/Catalog Number
M01263-5
BM5422 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-beta Actin Mouse Monoclonal Antibody catalog # M01263-5. Tested in WB application. This antibody reacts with Human, Monkey, Mouse, Rat, Goat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Actin Mouse Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01263-5)
Host
Mouse
Contents
Mouse IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
17A11
Isotype
IgG2b
Immunogen
A synthesized peptide derived from human beta Actin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01263-5 is reactive to ACTB in Goat, Human, Monkey, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated molecular weight
41737 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01263-5 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:3000-1:10000
Positive Control
WB: Hela, Human fetal kidney, 3T3 cell, PC-12 cell, COS-1 cell, Goat muscle
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41737 MW
Superfamily
actin family
Alternative Names
Actin, cytoplasmic 1;Beta-actin;Actin, cytoplasmic 1, N-terminally processed;ACTB; ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Actin Mouse Monoclonal Antibody (M01263-5)
Hello CJ!
M01263-5 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Curcumin suppresses neuroinflammation to protect neurons by preventing NLRP3 inflammasome activation:
Effects of L-theanine on intestinal morphology, barrier function, and MAPK signaling pathways in diquat-challenged piglets
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