Product Info Summary
SKU: | A01263-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Beta-actin ACTB Antibody
View all beta-Actin Antibodies
SKU/Catalog Number
A01263-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Beta-actin ACTB Antibody (Catalog # A01263-1). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Beta-actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Beta-actin ACTB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01263-1)
Host
Rabbit
Contents
Beta-actin Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Beta-actin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Beta-actin. The immunogen is located within the last 50 amino acids of Beta-actin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of SDHD are known to exist; this antibody will detect the two longest isoforms.
Reactive Species
A01263-1 is reactive to ACTB in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
41737 MW
Background of beta-Actin
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01263-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Beta-actin antibody can be used for the detection of Beta-actin by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of beta-actin in HeLa cell lysate with beta-actin antibody at (A) 0.5
Click image to see more details
Immunocytochemistry of beta-actin in HeLa cells with beta-actin antibody at 10 μg/mL.
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Immunofluorescence of Beta-actin in HeLa cells with Beta-actin antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41737 MW
Superfamily
actin family
Alternative Names
BRWS1, PS1TP5BP1, Actin, cytoplasmic 1, Beta-actin,b-actin ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beta-actin ACTB Antibody (A01263-1)
Hello CJ!
A01263-1 has been cited in 78 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cui J,Xu H,Yu J,Li Y,Chen Z,Zou Y,Zhang X,Du Y,Xia J,Wu J.IL-4 inhibits regulatory T cells differentiation by HDAC9-mediated epigenetic regulation.Cell Death Dis.2021 May 18;12(6):501.doi:10.1038/s41419-021-03769-7.PMID:34006836.
Species: Mouse
A01263-1 usage in article: APP:WB, SAMPLE:T CELL, DILUTION:1:1000
Hu J,Cai D,Zhao Z,Zhong G-C and Gong J (2021) Suppression of Heterogeneous Nuclear Ribonucleoprotein C Inhibit Hepatocellular Carcinoma Proliferation, Migration, and Invasion via Ras/MAPK Signaling Pathway.Front.Oncol.11:659676.doi:10.3389/fonc.2021.659676
Species: Human,Mouse
A01263-1 usage in article: APP:WB, SAMPLE:HCC TISSUE, DILUTION:1:2000
Li D,Liu J,Yang C,Tian Y,Yin C,Hu L,Chen Z,Zhao F,Zhang R,Lu A,Zhang G,Qian A.Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging.Theranostics.2021 Mar 20;11(11):5585-5604.doi:10.7150/thno.54477. PMID:33859765;PMCID:PMC8039942.
Species: Human,Mouse
A01263-1 usage in article: APP:WB, SAMPLE:HFOB1.19 CELL AND FEMUR TISSUE, DILUTION:1:1000
Xie J,Li L,Deng S,Chen J,Gu Q,Su H,Wen L,Wang S,Lin C,Qi C,Zhang Q,Li J,He X,Li W,Wang L,Zheng L.Slit2/Robo1 Mitigates DSS-induced Ulcerative Colitis by Activating Autophagy in Intestinal Stem Cell.Int J Biol Sci.2020 Apr 6;16(11):1876-1887.doi:10.7150/ijbs.42331.PMID:32398956;PMCID:PMC7211176.
Species: Mouse
A01263-1 usage in article: APP:WB, SAMPLE:COLON CRYPTS, DILUTION:1:10000
Wang L,Xiong X,Zhang X,Ye Y,Jian Z,Gao W,Gu L.Sodium Tanshinone IIA Sulfonate Protects Against Cerebral Ischemia-reperfusion Injury by Inhibiting Autophagy and Inflammation.Neuroscience.2020 Aug 10;441:46-57.doi:10.1016/j.neuroscience.2020.05.054.Epub 202
Species: Mouse
A01263-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:2000
xiangrong chen,Yile Zeng,Fan Wang et al.ADAM17 Aggravates the Inflammatory Response by Modulating Microglia Polarization Through the TGF-β1/Smad Pathway Following Experimental Traumatic Brain Injury,04 February 2021,PREPRINT (Version 1) available at
Species: Rat,Mouse
A01263-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE AND BV2 CELL, DILUTION:1:5000
Gu W,Shi Z,Song G,Zhang H.MicroRNA-199-3p up-regulation enhances chondrocyte proliferation and inhibits apoptosis in knee osteoarthritis via DNMT3A repression.Inflamm Res.2021 Jan 12.doi:10.1007/s00011-020-01430-1.Epub ahead of print.PMID:33433641.
Species: Rat
A01263-1 usage in article: APP:WB, SAMPLE:CHONDROCYTE, DILUTION:1:2500
Meng J,Su R,Wang L,Yuan B,Li L.2021.Inhibitory effect and mechanism of action (MOA) of hirsutine on the proliferation of T-cell leukemia Jurkat clone E6-1 cells.PeerJ 9:e10692 https://doi.org/10.7717/peerj.10692
Species: Human
A01263-1 usage in article: APP:WB, SAMPLE:JURKAT CLONE E6 CELL, DILUTION:1:1000
Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973. doi:10.1155/2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
A01263-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:1000
Fan J,He M,Wang CJ,Zhang M. Gadolinium Chloride Inhibits the Production of Liver Interleukin-27 and Mitigates Liver Injury in the CLP Mouse Model. Mediators Inflamm. 2021 Jan 29;2021:2605973.doi:10.1155/ 2021/2605973.PMID:33564275;PMCID:PMC7867451.
Species: Mouse
A01263-1 usage in article: APP:WB, SAMPLE:LIVER TISSUE, DILUTION:1:1000
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