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Product Info Summary
| SKU: | MA1115 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)
View all beta-Actin Antibodies
SKU/Catalog Number
MA1115
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) catalog # MA1115. Tested in IHC, WB applications. This antibody reacts with Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1115)
Host
Mouse
Contents
Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
Clonality
Monoclonal
Clone Number
AC-15
Isotype
Mouse IgG1
Immunogen
slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1115 is reactive to Actb in Chicken, Human, Monkey, Mouse, Pig, Rabbit, Rat
Reconstitution
Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
41737 MW
Background of beta-Actin
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1115 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.05-0.1μg/ml, Human, mouse, rat, pig, monkey, rabbit, chicken
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, mouse, rat, By Heat
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, monkey kidney tissue, monkey liver tissue, rat brain tissue, rat PC-12 whole cell, mouse brain tissue, mouse ANA-1 whole cell, rabbit intestine tissue, rabbit intestine tissue, pig intestine tissue
IHC: Mouse Intestine Tissue, Rat Intestine Tissue, Human Lung Cancer Tissue, Human Intestinal Cancer Tissue, Human Mammary Cancer Tissue
Validation Images & Assay Conditions
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Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Mouse Intestine Tissue
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Figure 1. Western blot analysis of beta-Actin using anti-beta-Actin antibody (MA1115).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: monkey kidney tissue lysates,
Lane 4: monkey liver tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse ANA-1 whole cell lysates,
Lane 9: rabbit intestine tissue lysates,
Lane 10: rabbit intestine tissue lysates,
Lane 11: pig intestine tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-beta-Actin antigen affinity purified monoclonal antibody (Catalog # MA1115) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for beta-Actin at approximately 42KD. The expected band size for beta-Actin is at 42KD.
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Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Rat Intestine Tissue
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Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Human Lung Cancer Tissue
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Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-beta-Actin antibody (monoclonal), MA1115, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For Actb (Source: Uniprot.org, NCBI)
Gene Name
Actb
Full Name
Actin, cytoplasmic 1
Weight
41737 MW
Superfamily
actin family
Alternative Names
Actin, cytoplasmic 1;Beta-actin;Actin, cytoplasmic 1, N-terminally processed;Actb; ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Actb, check out the Actb Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Actb: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15) (MA1115)
Hello CJ!
MA1115 has been cited in 683 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
HMGB1-induced endothelial cell pyroptosis is involved in systemic inflammatory response syndrome following radiofrequency ablation of hepatic hemangiomas
Rat bone mesenchymal stem cells exert antiproliferative effects on nicotine‑exposed T cells via iNOS production
Oxyresveratrol Inhibits IL-1β-Induced Inflammation via Suppressing AKT and ERK1/2 Activation in Human Microglia, HMC3
Artocarpus lakoocha Extract Inhibits LPS-Induced Inflammatory Response in RAW 264.7 Macrophage Cells
Kaempferia parviflora Extract Inhibits STAT3 Activation and Interleukin-6 Production in HeLa Cervical Cancer Cells
Inhibiting FAK–Paxillin Interaction Reduces Migration and Invadopodia-Mediated Matrix Degradation in Metastatic Melanoma Cells
Ischemic postconditioning reduced myocardial ischemia-reperfusion injury: The roles of melatonin and uncoupling protein 3
Boesenbergia rotunda extract accelerates human keratinocyte proliferation through activating ERK1/2 and PI3K/Akt kinases
Physiological and pharmacological effects of melatonin on remote ischemic perconditioning after myocardial ischemia‐reperfusion injury in rats: Role of Cybb, Fas, NfκB, Irisin signaling pathway
Species: Rat
Liu ZZ,Han ZD,Liang YK,Chen JX,Wan S,Zhuo YJ,Cai ZD,Deng YL,Lin ZY,Mo RJ,He HC,Zhong WD.TRIB1 induces macrophages to M2 phenotype by inhibiting IKB-zeta in prostate cancer.Cell Signal.2019 Jul;59:152-162.doi:10.1016/j.cellsig.2019.03.017.Epub 2019 Mar 26.PMID:30926388.
Species: Human,Mouse
MA1115 usage in article: APP:WB, SAMPLE:PC3 CELL,DU145 CELL AND THP-1 CELL, DILUTION:1:3000
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Customer Q&As
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7 Customer Q&As for Anti-Beta-Actin Actb Antibody (Monoclonal, AC-15)
Question
Is a blocking peptide available for product anti-Beta-Actin antibody (Monoclonal, AC-15) (MA1115)?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We do provide the blocking peptide for product anti-Beta-Actin antibody (Monoclonal, AC-15) (MA1115). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-25
Question
We are currently using anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
The anti-Beta-Actin antibody (Monoclonal, AC-15) (MA1115) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-19
Question
I see that the anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-03
Question
Is this MA1115 anti-Beta-Actin antibody (Monoclonal, AC-15) reactive to the isotypes of ACTB?
Verified Customer
Verified customer
Asked: 2019-01-07
Answer
The immunogen of MA1115 anti-Beta-Actin antibody (Monoclonal, AC-15) is slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-01-07
Question
I have attached the WB image, lot number and protocol we used for cajal-retzius cell fetal brain cortex using anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-30
Question
Will anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 work on feline IHC with b-cell lymphoma?
Verified Customer
Verified customer
Asked: 2018-01-12
Answer
Our lab technicians have not tested anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Beta-Actin antibody (Monoclonal, AC-15) MA1115 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline b-cell lymphoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-12
Question
I was wanting to use your anti-Beta-Actin antibody (Monoclonal, AC-15) for IHC for human cajal-retzius cell fetal brain cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cajal-retzius cell fetal brain cortex identification?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
It shows on the product datasheet, MA1115 anti-Beta-Actin antibody (Monoclonal, AC-15) has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cajal-retzius cell fetal brain cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-24
