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Product Info Summary
| SKU: | M02417 |
|---|---|
| Size: | 100µg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, IF, IHC, WB |
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Product info
Product Name
Anti-APRIL (human) Monoclonal Antibody (Aprily-8)
View all APRIL/TNFSF13 Antibodies
SKU/Catalog Number
M02417
Size
100µg
Form
Liquid. In PBS containing 0.02% sodium azide.
Description
Boster Bio Anti-APRIL (human) Monoclonal Antibody (Aprily-8) catalog # M02417. Tested in Flow Cytometry, IP, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APRIL (human) Monoclonal Antibody (Aprily-8) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02417)
Host
Mouse
Contents
Liquid. In PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Aprily-8
Isotype
IgG1, kappa
Immunogen
Recombinant human APRIL (aa 93-233) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M02417 is reactive to TNFSF13 in Human
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
58827 MW
Background of APRIL/TNFSF13
This monoclonal antibody recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02417 is guaranteed for Flow Cytometry, IP, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Optimal conditions must be determined individually for each application.
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TNFSF13 using anti-TNFSF13 antibody (M02417).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNFSF13 antigen affinity purified polyclonal antibody (Catalog # M02417) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for TNFSF13.
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Figure 2: FACS analysis of cells with MAb to APRIL (Aprily-8).Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5x105) were incubated on ice for 30 min. in 50
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Figure 3: Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8.Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4% formaldehyde 5 min. at RT. After a wash in PBS, samples were dehydrated by washes in 60%, 80%, 90%, 100% EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50°C. Slides were then successively washed 2x 10 min. in xylol, 2x 10 min. in 100% ethanol, and then treated 10 min. in 100% methanol/0.6% H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90%, 80%, 60% ethanol
Protein Target Info & Infographic
Gene/Protein Information For TNFSF13 (Source: Uniprot.org, NCBI)
Gene Name
TNFSF13
Full Name
Tumor necrosis factor ligand superfamily member 13
Weight
58827 MW
Superfamily
tumor necrosis factor family
Alternative Names
Keratin, type I cytoskeletal 10;Cytokeratin-10;CK-10;Keratin-10;K10;KRT10;KPP; TNFSF13 APRIL, CD256, TALL-2, TALL2, TNLG7B, TRDL-1, UNQ383/PRO715, ZTNF2 TNF superfamily member 13 tumor necrosis factor ligand superfamily member 13|TNF- and APOL-related leukocyte expressed ligand 2|a proliferation-inducing ligand|tumor necrosis factor (ligand) superfamily, member 13|tumor necrosis factor ligand 7B|tumor necrosis factor superfamily member 13|tumor necrosis factor-like protein ZTNF2|tumor necrosis factor-related death ligand-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFSF13, check out the TNFSF13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APRIL (human) Monoclonal Antibody (Aprily-8) (M02417)
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