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Product Info Summary
| SKU: | EK1565 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat Sclerostin/SOST ELISA Kit PicoKine®
View all SOST/Sclerostin ELISA kits
SKU/Catalog Number
EK1565
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat Sclerostin/SOST ELISA Kit PicoKine® (96 Tests). Quantitate Rat Sost in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat Sclerostin/SOST ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1565)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F29-Y213
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1565 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1565 is reactive to Sost in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1565 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SOST/Sclerostin
Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1565-CAP | Anti-Rat Sost Pre-coated 96-well strip microplate | 1 |
| EK1565-ST | Rat Sost Standard | 2 vials, 10 ng/tube |
| EK1565-DA | Rat Sost Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.080 | 0.109 | 0.138 | 0.181 | 0.285 | 0.543 | 1.219 | 2.315 |
Data Example Images
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Rat Sclerostin/SOST PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SOST/Sclerostin, Dilution ratio of 1:1, concentration in serum and plasma is around 400 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Sclerostin/SOST ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 33 | 214 | 472 | 33 | 234 | 430 |
| Standard deviation | 1.78 | 13.26 | 20.29 | 1.94 | 14.97 | 20.64 |
| CV (%) | 5.4% | 6.2% | 4.3% | 5.9% | 6.4% | 4.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SOST/Sclerostin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 33 | 34 | 37 | 35 | 34 | 1.47 | 4.3% |
| Sample 2 | 214 | 226 | 230 | 233 | 225 | 7.22 | 3.2% |
| Sample 3 | 472 | 504 | 525 | 455 | 489 | 27.23 | 5.5% |
Protein Target Info & Infographic
Gene/Protein Information For Sost (Source: Uniprot.Org, NCBI)
Gene Name
Sost
Full Name
Sclerostin
Weight
23974 MW
Superfamily
sclerostin family
Alternative Names
Sclerostin;Sost; SOST CDD, DAND61, VBCH, SOST sclerostin sclerostin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Sost, check out the Sost Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Sost: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat Sclerostin/SOST ELISA Kit PicoKine® (EK1565)
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Customer Q&As
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7 Customer Q&As for Rat Sclerostin/SOST ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-08-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-08-24
Question
Q: if the enzyme conjugated SOST antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SOST antigen?
C. Lewis
Verified customer
Asked: 2019-11-24
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-11-24
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring SOST using the EK1565 Rat SOST Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-09-13
Question
Q: can I use citrate plasma as samples in Rat SOST Picokine® ELISA Kit (Catalog # EK1565)?
V. Wilson
Verified customer
Asked: 2018-05-14
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of SOST causing degradation of its protein structure. SOST may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the SOST ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-05-14
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of SOST in Rat cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
E. Yang
Verified customer
Asked: 2016-10-17
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SOST ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2016-10-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-03-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-03-28
Question
Q: What is the optimal O.D. value for SOST ELISA kit? I used your SOST ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SOST even though the O.D. values are not very high?
P. Clark
Verified customer
Asked: 2015-01-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SOST ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SOST that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2015-01-05
