Anti-SOST Antibody (N-term)

Product Name

Anti-SOST Antibody (N-term)

View all SOST/Sclerostin Antibodies

SKU/Catalog Number

A03551-1

Size

50 µl

Description

Boster Bio Anti-SOST Antibody (N-term) (Catalog # A03551-1). Tested in IHC-P-Leica, WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-SOST Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03551-1)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SOST.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03551-1 is reactive to SOST in Human, Mouse, Rat

Applications

A03551-1 is guaranteed for WB, IHC-P-Leica Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

24031 Da

Background of SOST/Sclerostin

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:2000
IHC-P-Leica: 1:500

Validation Images & Assay Conditions

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Western blot analysis of lysates from mouse liver, mouse lung, rat kidney tissue lysate (from left to right), using SOST Antibody (N-term). A03551-1 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.

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All lanes : Anti-SOST Antibody (N-term) at 1:2000 dilution
Lane 1: mouse lung lysates
Lane 2: human liver lysates
Lane 3: Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 24 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

All lanes : Anti-SOST Antibody (N-term) at 1:1000 dilution
Lane 1: human kidney lysates
Lane 2: human liver lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 24 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

Anti-SOST Antibody (N-term) at 1:2000 dilution + Recombinant human SOST protein lysate
Lysates/proteins at 20 ng per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 24 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded Human kidney tissue using A03551-1 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Gene/Protein Information For SOST (Source: Uniprot.org, NCBI)

Alternative Names

sclerostin; SOST; VBCHsclerosteosis SOST CDD, DAND61, VBCH, SOST sclerostin sclerostin

For more info on SOST, check out the SOST Infographic

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In this infographic, you will see the following information for SOST: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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