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Product Info Summary
| SKU: | EK1299 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat CD36/SR-B3/GP4 ELISA Kit PicoKine®
View all CD36/SR-B3 ELISA kits
SKU/Catalog Number
EK1299
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat CD36/SR-B3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cd36 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat CD36/SR-B3/GP4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1299)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: G30-K439
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1299 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1299 is reactive to Cd36 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1299 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD36/SR-B3
CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1299-CAP | Anti-Rat Cd36 Pre-coated 96-well strip microplate | 1 |
| EK1299-ST | Rat Cd36 Standard | 2 vials, 10 ng/tube |
| EK1299-DA | Rat Cd36 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.072 | 0.182 | 0.319 | 0.435 | 0.689 | 1.087 | 1.539 | 2.037 |
Data Example Images
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Rat CD36/SR-B3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD36/SR-B3, Dilution ratio of 1:1, concentration in serum and plasma is 352-2152 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat CD36/SR-B3/GP4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 297 | 1163 | 5833 | 317 | 1160 | 5856 |
| Standard deviation | 16.92 | 91.87 | 425.8 | 19.65 | 92.8 | 462.62 |
| CV (%) | 5.7% | 7.9% | 7.3% | 6.2% | 8% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD36/SR-B3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 297 | 267 | 314 | 299 | 294 | 17.04 | 5.7% |
| Sample 2 | 1163 | 1212 | 1289 | 1172 | 1209 | 49.73 | 4.1% |
| Sample 3 | 5833 | 5270 | 6084 | 5144 | 5582 | 388.64 | 6.9% |
Protein Target Info & Infographic
Gene/Protein Information For Cd36 (Source: Uniprot.Org, NCBI)
Gene Name
Cd36
Full Name
Platelet glycoprotein 4
Weight
52731 MW
Superfamily
CD36 family
Alternative Names
Platelet glycoprotein 4;Adipocyte membrane protein;Fatty acid translocase;Fatty acid transport protein;Glycoprotein IIIb;GPIIIB;PAS IV;PAS-4;Platelet glycoprotein IV;GPIV;CD36;Cd36;Fat; CD36 BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3 CD36 molecule platelet glycoprotein 4|CD36 (collagen type I receptor, thrombospondin receptor)|CD36 molecule (thrombospondin receptor)|GPIIIB|PAS IV|PAS-4 protein|cluster determinant 36|fatty acid translocase|glycoprotein IIIb|leukocyte differentiation CD36|platelet glycoprotein IV|scavenger receptor class B, member 3
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Cd36, check out the Cd36 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd36: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat CD36/SR-B3/GP4 ELISA Kit PicoKine® (EK1299)
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No publications found for EK1299
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Rat CD36/SR-B3/GP4 ELISA Kit PicoKine®
Question
Q: how can I thaw whole blood sample for CD36 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-06-18
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD36 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-06-18
Question
I would like to know if your product EK1299 kit detects endogenous expression.
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
The EK1299 kit has been validated and guaranteed to detect endogenous expression using IHC and WB.
Boster Scientific Support
Answered: 2019-12-11
Question
Q: how to analyze ELISA data? I measured CD36 level in cell lysates.
R. Adams
Verified customer
Asked: 2019-04-20
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-04-20
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CD36 in Rat cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-07-08
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD36 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-07-08
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD36 using the EK1299 Rat CD36 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-09
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2017-03-09
Question
Q: Can CD36 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2017-01-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2017-01-01
Question
Q: What is the optimal O.D. value for CD36 ELISA kit? I performed your CD36 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD36 even though the O.D. values are not very high?
K. Parker
Verified customer
Asked: 2014-02-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD36 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD36 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2014-02-14
