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Product Info Summary
| SKU: | M01189 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-CD36/Sr B3 Rabbit Monoclonal Antibody
View all CD36/SR-B3 Antibodies
SKU/Catalog Number
M01189
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD36/Sr B3 Rabbit Monoclonal Antibody catalog # M01189. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD36/Sr B3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01189)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GFD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD36
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01189 is reactive to CD36 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
38 kDa
Calculated molecular weight
53053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01189 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
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Western blot analysis of CD36 expression in 3T3 cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For CD36 (Source: Uniprot.org, NCBI)
Gene Name
CD36
Full Name
Platelet glycoprotein 4
Weight
53053 MW
Superfamily
CD36 family
Alternative Names
Platelet glycoprotein 4;Fatty acid translocase;FAT;Glycoprotein IIIb;GPIIIB;Leukocyte differentiation antigen CD36;PAS IV;PAS-4;Platelet collagen receptor;Platelet glycoprotein IV;GPIV;Thrombospondin receptor;CD36;CD36;GP3B, GP4; CD36 BDPLT10, CHDS7, FAT, GP3B, GP4, GPIV, PASIV, SCARB3 CD36 molecule platelet glycoprotein 4|CD36 (collagen type I receptor, thrombospondin receptor)|CD36 molecule (thrombospondin receptor)|GPIIIB|PAS IV|PAS-4 protein|cluster determinant 36|fatty acid translocase|glycoprotein IIIb|leukocyte differentiation CD36|platelet glycoprotein IV|scavenger receptor class B, member 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD36, check out the CD36 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD36: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD36/Sr B3 Rabbit Monoclonal Antibody (M01189)
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No publications found for M01189
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD36/Sr B3 Rabbit Monoclonal Antibody
Question
Does anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 work for WB with liver?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
According to the expression profile of liver, CD36 is highly expressed in liver. So, it is likely that anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 will work for WB with liver.
Boster Scientific Support
Answered: 2020-01-17
Question
Is a blocking peptide available for product anti-CD36/Sr B3 Rabbit Monoclonal antibody (M01189)?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
We do provide the blocking peptide for product anti-CD36/Sr B3 Rabbit Monoclonal antibody (M01189). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-14
Question
Will M01189 anti-CD36/Sr B3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Banerjee
Verified customer
Asked: 2019-11-14
Answer
As indicated on the product datasheet, M01189 anti-CD36/Sr B3 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-14
Question
I would like to test anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 on rat liver for research purposes, then I may be interested in using anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-05
Answer
The products we sell, including anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-05
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-14
Question
Our lab used your anti-CD36/Sr B3 Rabbit Monoclonal antibody for WB on adipose tissue a few months ago. I am using human, and We intend to use the antibody for IP next. We want examining adipose tissue as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
I took a look at the website and datasheets of our anti-CD36/Sr B3 Rabbit Monoclonal antibody and it appears that M01189 has been validated on human in both WB and IP. Thus M01189 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-03
Question
Here is the WB image, lot number and protocol we used for liver using anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-13
Question
I was wanting to use your anti-CD36/Sr B3 Rabbit Monoclonal antibody for WB for rat liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat liver identification?
E. Dhar
Verified customer
Asked: 2018-04-04
Answer
It shows on the product datasheet, M01189 anti-CD36/Sr B3 Rabbit Monoclonal antibody has been validated for IP, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-04-04
Question
We were satisfied with the WB result of your anti-CD36/Sr B3 Rabbit Monoclonal antibody. However we have observed positive staining in skeletal muscle cell membrane using this antibody. Is that expected? Could you tell me where is CD36 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-03-28
Answer
From literature, skeletal muscle does express CD36. Generally CD36 expresses in cell membrane. Regarding which tissues have CD36 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Liver, Pubmed ID: 19159218
Milk, Pubmed ID: 18780401
Placenta, Pubmed ID: 2473841
Platelet, Pubmed ID: 16263699, 2468669, 11668637, 7505064, 7518447, 12853948
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-03-28
Question
I have a question about product M01189, anti-CD36/Sr B3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01189 anti-CD36/Sr B3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-20
Question
Is this M01189 anti-CD36/Sr B3 Rabbit Monoclonal antibody reactive to the isotypes of CD36?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
The immunogen of M01189 anti-CD36/Sr B3 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD36 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-02
Question
We have seen staining in mouse adipocyte. Do you have any suggestions? Is anti-CD36/Sr B3 Rabbit Monoclonal antibody supposed to stain adipocyte positively?
Verified Customer
Verified customer
Asked: 2017-07-31
Answer
Based on literature adipocyte does express CD36. Based on Uniprot.org, CD36 is expressed in adipose tissue, placenta, platelet, skeletal muscle, adipocyte, milk, liver, among other tissues. Regarding which tissues have CD36 expression, here are a few articles citing expression in various tissues:
Adipocyte, Pubmed ID: 15242332
Liver, Pubmed ID: 19159218
Milk, Pubmed ID: 18780401
Placenta, Pubmed ID: 2473841
Platelet, Pubmed ID: 16263699, 2468669, 11668637, 7505064, 7518447, 12853948
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-07-31
Question
Does anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 work on bovine IP with adipocyte?
Verified Customer
Verified customer
Asked: 2017-07-20
Answer
Our lab technicians have not validated anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine adipocyte in IP, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-20
Question
I see that the anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-03
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-03
Question
Do you have a BSA free version of anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 available?
G. Roberts
Verified customer
Asked: 2015-11-04
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-11-04
Question
We are currently using anti-CD36/Sr B3 Rabbit Monoclonal antibody M01189 for mouse tissue, and we are happy with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
K. Carter
Verified customer
Asked: 2014-07-25
Answer
The anti-CD36/Sr B3 Rabbit Monoclonal antibody (M01189) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-07-25
