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Product Info Summary
| SKU: | EK1623 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat FABP3 ELISA Kit PicoKine®
View all FABP3/H-FABP ELISA kits
SKU/Catalog Number
EK1623
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat FABP3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Fabp3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat FABP3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1623)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A2-A133
Sensitivity
<10 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1623 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1623 is reactive to Fabp3 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1623 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FABP3/H-FABP
Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1623-CAP | Anti-Rat Fabp3 Pre-coated 96-well strip microplate | 1 |
| EK1623-ST | Rat Fabp3 Standard | 2 vials, 10 ng/tube |
| EK1623-DA | Rat Fabp3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 13000 | 3000 | 6000 |
| O.D. | 0.075 | 0.166 | 0.229 | 0.356 | 0.588 | 1.016 | 1.722 | 2.411 |
Data Example Images
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Rat FABP3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FABP3/H-FABP, Dilution ratio of 1:400, concentration in serum and plasma is around 700 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat FABP3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 131 | 866 | 2555 | 124 | 868 | 2555 |
| Standard deviation | 6.02 | 54.55 | 158.41 | 7.19 | 60.76 | 181.4 |
| CV (%) | 4.6% | 7%% | 6.2% | 5.8% | 7% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FABP3/H-FABP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 131 | 127 | 121 | 131 | 127 | 4.09 | 3.2% |
| Sample 2 | 866 | 923 | 850 | 898 | 884 | 28.26 | 3.1% |
| Sample 3 | 2555 | 2241 | 2468 | 2352 | 2404 | 118.5 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For Fabp3 (Source: Uniprot.Org, NCBI)
Gene Name
Fabp3
Full Name
Fatty acid-binding protein, heart
Weight
14.858kDa
Superfamily
calycin superfamily
Alternative Names
Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP; Fabp3 FABP3 FABP11, H-FABP, M-FABP, MDGI, O-FABP fatty acid binding protein 3 fatty acid-binding protein, heart|epididymis secretory sperm binding protein|fatty acid binding protein 11|fatty acid binding protein 3, muscle and heart|heart-type fatty acid-binding protein|mammary-derived growth inhibitor|muscle fatty acid-binding protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Fabp3, check out the Fabp3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fabp3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat FABP3 ELISA Kit PicoKine® (EK1623)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Rat FABP3 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
D. Wilson
Verified customer
Asked: 2020-03-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-03-21
Question
Q: how can I thaw whole blood sample for FABP3 ELISA after freezing?
T. Kulkarni
Verified customer
Asked: 2019-11-27
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FABP3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-11-27
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification FABP3 using the EK1623 Rat FABP3 Picokine® ELISA Kit?
J. Allen
Verified customer
Asked: 2019-09-28
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2019-09-28
Question
Q: how to analyze ELISA data? I have obtained FABP3 level in plasma.
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-06-10
Question
Q: is it okay to use citrate plasma as samples in Rat FABP3 Picokine® ELISA Kit (Catalog # EK1623)?
T. Bhatt
Verified customer
Asked: 2018-10-10
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of FABP3 causing disruption of its protein structure. FABP3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the FABP3 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-10-10
Question
Q: can you recommend the dilution ratio of serum samples for detection of FABP3 in Rat plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-01-22
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FABP3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-01-22
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2015-09-22
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-09-22
