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Product Info Summary
| SKU: | EKC2496 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human |
| Application: | ELISA |
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Product info
Product Name
PKR (Phospho-Thr446) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC2496
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The PKR (Phospho-Thr446) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PKR (Phospho-Thr446) protein expression profile in cells. The kit can be used for measuring the relative amounts of PKR (Phospho-Thr446) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PKR (Phospho-Thr446).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
PKR (Phospho-Thr446) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC2496)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC2496 is reactive to EIF2AK2 in Human samples
Application Guarantee
EKC2496 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-PKR Phospho-Thr446 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-PKR Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from NIH-3T3 cells treated with IFN 2500U/ml 30'
Protein Target Info & Infographic
Gene/Protein Information For EIF2AK2 (Source: Uniprot.Org, NCBI)
Gene Name
EIF2AK2
Full Name
Interferon-induced, double-stranded RNA-activated protein kinase
Weight
62094 MW
Superfamily
protein kinase superfamily
Alternative Names
Interferon-induced, double-stranded RNA-activated protein kinase;2.7.11.1;Eukaryotic translation initiation factor 2-alpha kinase 2;eIF-2A protein kinase 2;Interferon-inducible RNA-dependent protein kinase;P1/eIF-2A protein kinase;Protein kinase RNA-activated;PKR;Protein kinase R ;Tyrosine-protein kinase EIF2AK2;2.7.10.2;p68 kinase;EIF2AK2;PKR, PRKR; EIF2AK2 EIF2AK1, LEUDEN, PKR, PPP1R83, PRKR eukaryotic translation initiation factor 2 alpha kinase 2 interferon-induced, double-stranded RNA-activated protein kinase|P1/eIF-2A protein kinase|double stranded RNA activated protein kinase|eIF-2A protein kinase 2|interferon-inducible elF2alpha kinase|p68 kinase|protein kinase R|protein kinase, interferon-inducible double stranded RNA dependent|protein phosphatase 1, regulatory subunit 83|tyrosine-protein kinase EIF2AK2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on EIF2AK2, check out the EIF2AK2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EIF2AK2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For PKR (Phospho-Thr446) Colorimetric Cell-Based ELISA Kit (EKC2496)
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Customer Q&As
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3 Customer Q&As for PKR (Phospho-Thr446) Colorimetric Cell-Based ELISA Kit
Question
Did you use a peptide or full-length protein in the ready-to-use substrate of EKC2496?
Verified customer
Asked: 2022-05-23
Answer
The ready to use substrate contains 3,3',5,5'-tetramethylbenzidine (TMB). The immunogen of the antibodies for the PKR (Phospho-Thr446) Colorimetric Cell-Based ELISA Kit (EKC2496) is produced from peptide.
Boster Scientific Support
Answered: 2022-05-24
Question
Are the plates that are included in EKC2496 treated with somenthing special?
Verified customer
Asked: 2021-11-04
Answer
The plates included in the PKR (Phospho-Thr446) Colorimetric Cell-Based ELISA Kit (EKC2496) are standard tissue culture treated plates (Corning Costar #3595).
Boster Scientific Support
Answered: 2021-11-04
Question
Are the plates that are included in EKC2496 treated with somenthing special?
Verified customer
Asked: 2021-11-04
Answer
The plates included in the PKR (Phospho-Thr446) Colorimetric Cell-Based ELISA Kit (EKC2496) are standard tissue culture treated plates (Corning Costar #3595).
Boster Scientific Support
Answered: 2021-11-04
