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Product Info Summary
| SKU: | EK0515 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (EDTA) and urine. |
Product info
Product Name
Mouse TGF Beta 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0515
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TGF Beta 1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tgfb1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TGF Beta 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0515)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: CHO; Immunogen sequence: A279-S390
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0515 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0515 is reactive to Tgfb1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (EDTA) and urine.
Application Guarantee
EK0515 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TGFB1
Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects. TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation. TGFbeta1 is also an important mediator of bone remodeling. TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation. TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0515-CAP | Anti-Mouse Tgfb1 Pre-coated 96-well strip microplate | 1 |
| EK0515-ST | Mouse Tgfb1 Standard | 2 vials, 10 ng/tube |
| EK0515-DA | Mouse Tgfb1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.057 | 0.138 | 0.220 | 0.406 | 0.611 | 1.060 | 1.612 | 2.047 |
Data Example Images
Click image to see more details
Mouse TGF beta 1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TGFB1, Dilution ratio of 1:500, concentration in serum and plasma is 14-210 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TGF Beta 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 36 | 169 | 499 | 33 | 183 | 484 |
| Standard deviation | 1.47 | 11.66 | 31.43 | 1.55 | 12.99 | 34.36 |
| CV (%) | 4.1% | 6.9% | 7.1% | 4.7% | 7.1% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TGFB1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 36 | 39 | 42 | 38 | 38 | 2.16 | 5.6% |
| Sample 2 | 169 | 169 | 184 | 172 | 173 | 6.18 | 3.5% |
| Sample 3 | 499 | 442 | 521 | 490 | 488 | 28.85 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For Tgfb1 (Source: Uniprot.Org, NCBI)
Gene Name
Tgfb1
Full Name
Transforming growth factor beta-1 proprotein
Weight
44310 MW
Superfamily
TGF-beta family
Alternative Names
Transforming growth factor beta-1;TGF-beta-1;Latency-associated peptide;LAP;Tgfb1; TGFB1 CED, DPD1, IBDIMDE, LAP, TGF-beta1, TGFB, TGFbeta transforming growth factor beta 1 transforming growth factor beta-1 proprotein|TGF-beta-1|latency-associated peptide|prepro-transforming growth factor beta-1|transforming growth factor beta1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tgfb1, check out the Tgfb1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tgfb1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TGF Beta 1 ELISA Kit PicoKine® (EK0515)
Hello CJ!
EK0515 has been cited in 106 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Role of Macrophage Migration Inhibitory Factor (MIF) in Asthmatic Airway Remodeling
Tumor cell lysate induces the immunosuppression and apoptosis of mouse immunocytes
Effects of a Unique Combination of the Whole-Body Low Dose Radiotherapy with Inactivation of Two Immune Checkpoints and/or a Heat Shock Protein on the Transplantable Lung Cancer in Mice
Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro
Shikonin Prolongs Allograft Survival via Induction of CD4+FoxP3+ Regulatory T Cells
Macrophage Foam Cell-Targeting Immunization Attenuates Atherosclerosis
TLR4 signaling pathway in mouse Lewis lung cancer cells promotes the expression of TGF-β1 and IL-10 and tumor cells migration
The effects of Panax notoginseng saponins on the cytokines and peritoneal function in rats with peritoneal fibrosis
Neuroprotective effects of α-lipoic acid on long-term experimental autoimmune encephalomyelitis.
Branched Chain Amino Acids Protects Rat Mesangial Cells from High Glucose by Modulating TGF-β1 and BMP-7
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Mouse TGF Beta 1 ELISA Kit PicoKine®
Question
Was mouse urine validated for EK0515?
Verified customer
Asked: 2022-07-06
Answer
Our lab hasn't worked on urine samples using the Mouse TGF Beta 1 ELISA Kit PicoKine® (EK0515).
Boster Scientific Support
Answered: 2022-07-07
Question
Q: if the enzyme conjugated TGFB1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGFB1 antigen?
T. Phillips
Verified customer
Asked: 2020-11-10
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-10
Question
Q: Can TGFB1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Zhao
Verified customer
Asked: 2020-10-07
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-07
Question
Q: What is the optimal O.D. value for TGFB1 ELISA kit? I used your TGFB1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TGFB1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-07-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGFB1 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of TGFB1 that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2020-07-05
Question
Is your mouse monoclonal to TGF-beta in the kit specific to mouse, or will it cross with human TGF-beta or Bovine TGF-beta that may be present in the media from the FBS? Keyword: specificity
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
TGFb1 is conservative, EK0515 also cross reacts with human and bovine TGFb1.
Boster Scientific Support
Answered: 2019-11-26
Question
What does the EK0515 kit detect after sample´s activation?
Verified customer
Asked: 2019-11-11
Answer
The Mouse TGF Beta 1 ELISA Kit PicoKine® (EK0515) detects Active TGF beta after activating the sample.
Boster Scientific Support
Answered: 2019-11-12
Question
Does your mouse TGF-beta kit detect TGF-beta when in complex with its binding proteins or latent complex? Do I have to perform some kind of extraction or treat their supernatants with RIPA or other preparation to expose the actual free TGF-beta or will your monoclonal detect the TGF beta even when it is in the latent forms? Keyword: applications, protocol, detection
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
TGFβ1 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGFβ1. Please contact us for recommended protocol to activate the sample (if want to analyze the active form).
Boster Scientific Support
Answered: 2019-10-14
Question
What is a reference range for mouse serum samples? Keyword: experimental data
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
TGF-β1 level in mouse serum is about 80-123ng/ml.
Boster Scientific Support
Answered: 2019-09-26
Question
What is the protocol for performing EK0515 to test for the active form of TGF-b (acidification of the tissue homogenate sample)?
Verified customer
Asked: 2019-09-03
Answer
For the Mouse TGF Beta 1 ELISA Kit PicoKine™ (EK0515), please see the following protocol for activating tissue homogenate samples; 1. Mince tissue and then rinse tissue in ice-cold PBS. 2. Place minced tissue in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS(Volumes of PBS must be determined in relation to the amount of tissue present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no tissue chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 10.Add activating reagent into the supernatant pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Solution A: 1N HCl: add 8.33ml of 12N HCl into 91.67ml of H2O. Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml. Note: Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Please perform the endogenous biotin test first.
Boster Scientific Support
Answered: 2019-09-03
Question
Will EK0515 work for mouse tracheal tissue homogenates with samples approximately 4mm^3?
Verified customer
Asked: 2019-07-30
Answer
For the Mouse TGF Beta 1 ELISA Kit PicoKine™ (EK0515), the mouse tracheal tissue homogenates may work regardless of sample size as long as the endogenous biotin test has been done first.
Boster Scientific Support
Answered: 2019-07-31
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TGFB1 in Mouse urine? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
H. Perez
Verified customer
Asked: 2019-04-30
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGFB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-04-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-12-24
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
H. Martinez
Verified customer
Asked: 2018-09-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-09-02
Question
Q: can I use heparin plasma as samples in Mouse TGFB1 Picokine® ELISA Kit (Catalog # EK0515)?
W. Davis
Verified customer
Asked: 2018-08-14
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TGFB1 causing degradation of its protein structure. TGFB1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TGFB1 ELISA, treating samples with a number of anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-14
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TGFB1 using the EK0515 Mouse TGFB1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2017-07-25
Question
Thanks for the info. Would you be able to provide a protocol for performing the ELISA to test for the active form of TGF-b (acidification of the tissue homogenate sample)?
Verified Customer
Verified customer
Asked: 2015-03-11
Answer
Please see the following protocol for activating tissue homogenate samples 1. Mince tissue and then rinse tissue in ice-cold PBS. 2. Place minced tissue in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS(Volumes of PBS must be determined in relation to the amount of tissue present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no tissue chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 10.Add activating reagent into the supernatant pro rata, i.e. add 20?l of Solution A into 100?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6. Solution A: 1N HCl: add 8.33ml of 12N HCl into 91.67ml of H2O. Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml. Note: Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Please perform the endogenous biotin test first.
Boster Scientific Support
Answered: 2015-03-11
Question
I'm interested in purchasing the Mouse TGF Beta 1 PicoKine
Verified Customer
Verified customer
Asked: 2013-04-17
Answer
We haven't tested mouse tracheal tissue homogenates with EK0412. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2013-04-17
