TGFB1 Antibodies

Transforming growth factor beta-1 proprotein (TGFB1)

Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors.

It plays an important role in bone remodeling as it's a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in dedicated osteoblasts (By similarity). Stimulates sustained production of collagen through the activation of CREB3L1 by regulated intramembrane proteolysis (RIP) (PubMed:25310401).

Gene Information

Human
Gene Name:	TGFB1
Uniprot:	P01137
Entrez:	7040

Family

Belongs to:
TGF-beta family

Alternative Names

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1

Molecular Weight

Mass (kDA):
44.341 kDA

Genomic Context

Human
Location:	19q13.2
Sequence:	19; NC_000019.10 (41330323..41353922, complement)

Tissue Specificity

Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage.

Subcellular Localizations

[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted.

Diseases

• Fibrosis
• Malignant Neoplasms
• Neoplasms
• Inflammation
• Kidney Diseases
• Carcinoma
• Tissue Adhesions
• Hepatic Fibrosis
• Liver Cirrhosis
• Diabetic Nephropathy
• Diabetes Mellitus
• Hypertrophy
• Tumor Angiogenesis

• Neoplasm Metastasis
• Hypertensive Disease
• Cell Invasion
• Pulmonary Fibrosis
• Hyperplasia
• Malignant Paraganglionic Neoplasm
• Malignant Neoplasm Of Breast

Interacting Proteins

• APP
• DIP2A
• ENG
• FSTL1
• LRRC32

• MEOX2
• PSG1
• TGFBR1
• TGFBR2
• TGFBR3

• THBS1

Pathways

• Pathogenesis
• Cell Proliferation
• Secretion
• Wound Healing
• Cell Growth
• Angiogenesis
• Regeneration
• Cell Cycle
• Localization
• Excretion
• Cell Death
• Cell Adhesion
• Immune Response

• Inflammatory Response
• Cytokine Production
• Cell Migration
• Reverse Transcription
• Cell Differentiation
• Cell Activation
• Fibroblast Proliferation

PTMs

• Phosphorylation
• Cleavage
• Methylation
• Dephosphorylation
• Acetylation
• Nitration
• Glycosylation
• Phosphorothioation
• Ubiquitination
• Reduction

• Oxidation
• Demethylation
• Hydroxylation
• Farnesylation
• Sulphation
• Carboxylation
• Nitrosylation
• Amidation
• Acylation
• Prenylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/TGFB1

Best Uses Of The TGFB1 Marker From Boster Bio

If you are considering purchasing an Anti-TGF beta 1 TGFB1 Marker, you may need to read the below information first. This article will show you how to utilize this product in flow cytometry. We'll also explain how to utilize this marker for IHC. We'll also cover the best ways to share your results , and the best ways to make the most of your anti-TGF beta1 TGFB1 marker.

Marker TGFB1

The fibroblastic cell line BMAL-2 is an important component of the TGFB1 gene. The molecule also regulates the function of other cellular proteins, such as the interferon-like regulator. It is therefore crucial to understand the role this protein plays in cell function. We investigated the molecular mechanism of transforming Growth Factor-B within macrophages. We focused on the role of transforming growth factor-b in modulating the activity DNMT1, Pellino 1 and interferon regulatory Fact 5 (IRF5).

The TGFBR1 gene was first described by Vellucci and Reiss (1997), and was reported to be 31 kb long, having nine exons. The gene encodes the C terminal segment of a serine/threonine-kinase-domain. The region appears to be preserved among members of the gene family. Therefore, the TGFB1 gene can be a valuable marker to study TGFb-related diseaseslike cancer.

A variety of illnesses have been related to the TGFB1 gene, including Camurati-Engelmann that is associated with abnormally thick bone. The disease is characterised by muscle weakness and pain in the bones and can cause pressure on the brain, which can lead to neurological problems, including headaches and hearing loss. TGFB1 gene mutations can also cause a disease known as Camurati-Engelmann syndrome.

The TGF-b gene family is comprised of various signaling proteins that play a crucial role in the development, differentiation, and pathology of cells. It is found in every cell's nucleus, and is in contact with a variety of signaling proteins. These proteins communicate with two types of serine-threonine-kinase receptors. They phosphorylate effector SMAD proteins, which transfer to the nucleus and control transcription. Additionally, TGF-b proteins can be released into latent complexes which contain TGF-b binding proteins.

Anti-TGF beta 1 Antibody to TGFB1

Boster Bio Anti-TGF Beta 1 TGFB1 Clone5D2 from Boster Bio was purified using mouse ascites , and affinity removed. The anti-TGF beta 1 antibody reacts with Human. It is stable at -20degC one year and fourdegC for one month. It is available in vials that contain sodium chloride, trehalose and Na2HPO4 as the blocking protein.

There are three types of the beta cytokine that transforms growth factor. Each one is a different one with a specific purpose. It is typically overexpressed in advanced cancers and has been linked to poor prognoses. It hinders cell proliferation, causes apoptosis, promotes drug resistance, and controls the functions of several immune cells, including regulatory T cells. In fact, it may increase the effectiveness of certain cancer treatments, including immune checkpoint inhibitors, as well as other immunotherapy treatments.

TGF-b1 also regulates the differentiation and production of cytokines during T cells activation. The anti-TGFb1 antibody YM101 was found to block TGF-b1's effects by stopping the production of several cytokines. To determine if the antibody inhibits TGF-b1's effects, murine T cells were cultivated in Corning plates with 96 wells and cultured for four days at 37°C.

The expression of ECM genes was inhibited by KO cells that expressed SB431542. The effect was more severe in KO cells was more severe than WT cells. Inactivated TGFb hinders the production of latent TGFb. The cells with KO had a higher level of total collagen than WT cells. TGF-b inhibition reduced the level of total collagen and ECM genes.

Boster anti-TGF beta1 antibody is highly cross-reactive, and has exceptional sensitiveness to human TGFB1. The Boster antibody is tested on positive and negative samples, and confirmed using a corresponding Boster assay. It is a good choice to conduct further research once it has been tested. It is very affordable. The Boster's customer service is outstanding.

This test uses cell lysate. Cell lysate is harvested by using RIPA buffer. The BCA kit for measuring protein is used to determine the amount of protein. The samples are then mixed with Laemmli buffer and heated to 95°C for five minutes. Then, the immunoblotting process is performed using anti-Myl9and anti-Itga11 Anti-GAPDH-HRP, and anti-b-actin antibodies. The immunoblotted areas can be developed using a Pierce ECL western blotting substrat and imaged with a ChemiDoc MPI Imaging System.

Application of flow Cytometry

Immunophenotyping is one of the most popular applications of flowcytometry. This involves staining cells with antibodies that target antigens that are present on the cell's surface. Human Leukocyte Differentiation Workshops classify these antigens using their "cluster of differentiation" numbers. For instance, antigen number 3 is an antibody for the co-receptor for T cells known as CD3.

Utilizing a specific software program the cytometer can study the spectrum of two fluorescent dyes and determine the ratio of their light intensities. In modern flow cytometry, this program automatically calculates fluorescence compensation mathematical formulas and filters data, which enable researchers to identify cells of interest. This way, researchers are able to selectively observe the cells that are of interest and eliminate other particles. This process is referred to as Gating.

The fluorescence measurements can also provide qualitative or quantitative data about the particle's characteristics. Flow cytometers typically use two distinct fluorescence channels. There are many kinds of light sources that can be utilized. The type of detectors used will differ according to the manufacturer. In general, silicon photodiodes may be used to measure forward scatter. Photomultiplier tubes can be used to measure fluorescence or scatter. It is essential to comprehend the principles behind flow cytometry before you use it in immunology.

The fluidics systems play a vital role in flow cytometry. The fluidics system regulates the flow of the samples and prevents them from mixing and clogging the nozzle. Another major feature is hydrodynamic focus, which blocks the analysis of the analysis of a single cell. This technique is also commonly used in immunohistochemistry. This software lets scientists discern specific characteristics of a cell's surface and determine if it's an organ or tissue.

Flow cytometry works by detecting different physical and chemical properties in a sample. Fluids are placed in tubes and injected into the flowcytometer. The fluid then undergoes an Acoustic radiation pressure that focuses the analytes. The flow cytometer may measure up to six physical properties or components of a sample by using this technique. The technology can even analyze the analysis of tens of millions of cells in less than a minute!

IHC workflow

This IHC procedure was performed using a paraffin-embedded slides that were formalin-fixed using a monoclonal anti-rat antibody against mouse CD8a. After the slides were fixed and permeated complete slide images were captured using the Leica Biosciences Aperio AT2 slide scanner. The resulting images were analyzed using the HALO AreaQuantification algorithm, which was developed by Indica Labs.

To determine the subtypes of cell types, we analysed the mean staining intensity for the TGFB1 marker in epithelium of three clusters of patients to determine if there was a latent TGFB1. The expression of TGFB1 was inversely related to the presence of epithelial-associated macrophages, but not to patient characteristics. This study suggests that TGFB1 could be linked to the morphology of the tumor, but not to the severity of the tumor.

Despite the loss of myofibroblasts interferon-licensed CAFs induced an entirely new set of cells that are known as transcriptionally unique. These cells were distinguished from other CAF subsets because of their increased expression of CD73. The expression of this gene was enough to permit the detection of ilCAFs with flow cytometry. The ilCAFs could be distinguished easily from other subsets of CAF.