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Product Info Summary
| SKU: | EK1355 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse TFPI ELISA Kit PicoKine®
SKU/Catalog Number
EK1355
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TFPI ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tfpi in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TFPI ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1355)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: L29-N306
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1355 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1355 is reactive to Tfpi in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1355 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TFPI
TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus. TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1355-CAP | Anti-Mouse Tfpi Pre-coated 96-well strip microplate | 1 |
| EK1355-ST | Mouse Tfpi Standard | 2 vials, 10 ng/tube |
| EK1355-DA | Mouse Tfpi Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 125000 | 25000 | 5000 | 10,000 |
| O.D. | 0.019 | 0.068 | 0.104 | 0.219 | 0.380 | 0.769 | 1.299 | 1.957 |
Data Example Images
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Mouse TFPI PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TFPI, Dilution ratio of 1:1, concentration in serum and plasma is around 3 ng/ml..
Some articles we found to cite concentrations of TFPI in samples: 19422457 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TFPI ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 310 | 1506 | 4359 | 330 | 1630 | 4238 |
| Standard deviation | 20.15 | 99.39 | 217.95 | 22.44 | 123.88 | 216.13 |
| CV (%) | 6.5% | 6.6% | 5% | 6.8% | 7.6% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TFPI in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 310 | 324 | 315 | 312 | 315 | 5.35 | 1.6% |
| Sample 2 | 1506 | 1410 | 1529 | 1285 | 1432 | 96.14 | 6.7% |
| Sample 3 | 4359 | 4343 | 4246 | 3938 | 4221 | 169.29 | 4% |
Protein Target Info & Infographic
Gene/Protein Information For Tfpi (Source: Uniprot.Org, NCBI)
Gene Name
Tfpi
Full Name
Tissue factor pathway inhibitor
Weight
34987 MW
Alternative Names
Tissue factor pathway inhibitor;TFPI;Extrinsic pathway inhibitor;EPI;Lipoprotein-associated coagulation inhibitor;LACI;Tfpi; TFPI EPI, LACI, TFI1, TFPI tissue factor pathway inhibitor tissue factor pathway inhibitor|anti-convertin|extrinsic pathway inhibitor|tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tfpi, check out the Tfpi Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tfpi: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TFPI ELISA Kit PicoKine® (EK1355)
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Customer Q&As
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8 Customer Q&As for Mouse TFPI ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for TFPI ELISA kit? I performed your TFPI ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TFPI even though the O.D. values are not very high?
S. Varma
Verified customer
Asked: 2020-01-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TFPI ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TFPI that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-01-31
Question
Q: which procedure should I follow in order to thaw whole blood sample for TFPI ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TFPI for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-12-27
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-03
Question
Q: if the enzyme conjugated TFPI antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TFPI antigen?
Verified Customer
Verified customer
Asked: 2018-09-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-23
Question
Q: how do I analyze ELISA data? I have obtained TFPI level in cell lysates.
S. King
Verified customer
Asked: 2018-05-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-07
Question
Q: is it okay to use citrate plasma as samples in Mouse TFPI Picokine® ELISA Kit (Catalog # EK1355)?
W. Jackson
Verified customer
Asked: 2018-02-28
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of TFPI causing degradation of its protein structure. TFPI may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the TFPI ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-02-28
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Z. Robinson
Verified customer
Asked: 2016-10-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-10-11
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TFPI in Mouse cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
H. Edwards
Verified customer
Asked: 2014-11-17
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TFPI ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-11-17
