Click image to see more details
Product Info Summary
| SKU: | EK1393 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
Product info
Product Name
Human Serpin C1/Antithrombin-III ELISA Kit PicoKine®
View all Serpin C1/Antithrombin-III ELISA kits
SKU/Catalog Number
EK1393
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Serpin C1/Antithrombin-III ELISA Kit PicoKine® (96 Tests). Quantitate Human SERPINC1 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Serpin C1/Antithrombin-III ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1393)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: H33-K464
Sensitivity
<10 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1393 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1393 is reactive to SERPINC1 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
Application Guarantee
EK1393 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin C1/Antithrombin-III
Antithrombin III is the most important inhibitor of thrombin and other coagulation proteinases. It belongs to the serine proteinase inhibitor (serpin) superfamily of inhibitors and structurally related proteins, which contain reactive centers that have evolved to attract and entrap certain proteinases. Inherited antithrombin III deficiency (AT3D) is a risk factor for the early development of venous thromboembolism (THPH7). Antithrombin III regulates clot formation both by inhibiting thrombin activity directly and by interfering with earlier stages of the clotting cascade. By in situ hybridization and quantitative analysis of DNA dosage in carriers of chromosome 1 deletions, AT3 is mapped to 1q23-q25. The AT3 gene has 7 exons. It contains 9 complete and 1 partial repetitive ALU sequence elements, which occur in the introns of the gene at a higher frequency (about 22% of the intron sequence) than in the genome as a whole (about 5%).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1393-CAP | Anti-Human SERPINC1 Pre-coated 96-well strip microplate | 1 |
| EK1393-ST | Human SERPINC1 Standard | 2 vials, 20 ng/tube |
| EK1393-DA | Human SERPINC1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 | 20,000 |
| O.D. | 0.011 | 0.062 | 0.082 | 0.15 | 0.295 | 0.582 | 1.333 | 1.974 |
Data Example Images
Click image to see more details
Human Serpin C1/Antithrombin-III PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin C1/Antithrombin-III, Dilution ratio of 1:20000, concentration in serum and plasma is 57-176 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Serpin C1/Antithrombin-III ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 647 | 1973 | 8982 | 647 | 2050 | 9365 |
| Standard deviation | 28.46 | 153.89 | 377.24 | 34.29 | 174.25 | 552.53 |
| CV (%) | 4.4% | 7.8% | 4.2% | 5.3% | 8.5% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin C1/Antithrombin-III in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 647 | 649 | 673 | 636 | 651 | 13.49 | 2% |
| Sample 2 | 1973 | 1798 | 1681 | 1827 | 1819 | 103.99 | 5.7% |
| Sample 3 | 8982 | 8247 | 8966 | 9377 | 8893 | 407.68 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For SERPINC1 (Source: Uniprot.Org, NCBI)
Gene Name
SERPINC1
Full Name
Antithrombin-III
Weight
52602 MW
Superfamily
serpin family
Alternative Names
Antithrombin-III;ATIII;Serpin C1;SERPINC1;AT3;PRO0309; SERPINC1 AT3, AT3D, ATIII, ATIII-R2, ATIII-T1, ATIII-T2, THPH7 serpin family C member 1 antithrombin-III|serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1|serpin peptidase inhibitor clade C member 1|serpin peptidase inhibitor, clade C (antithrombin), member 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SERPINC1, check out the SERPINC1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SERPINC1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Serpin C1/Antithrombin-III ELISA Kit PicoKine® (EK1393)
Hello CJ!
No publications found for EK1393
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Human Serpin C1/Antithrombin-III ELISA Kit PicoKine®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Human Serpin C1/Antithrombin-III ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Human Serpin C1/Antithrombin-III ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for Serpin C1 ELISA kit? I used your Serpin C1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Serpin C1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-08-18
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin C1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Serpin C1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-08-18
Question
Q: if the enzyme conjugated Serpin C1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin C1 antigen?
Verified Customer
Verified customer
Asked: 2020-07-07
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-07-07
Question
Q: can I use heparin plasma as samples in Human Serpin C1 Picokine® ELISA Kit (Catalog # EK1393)?
H. Mangal
Verified customer
Asked: 2019-12-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Serpin C1 causing degradation of its protein structure. Serpin C1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Serpin C1 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-12-29
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Serpin C1 using the EK1393 Human Serpin C1 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-07-18
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-07-18
Question
Q: how do I analyze ELISA data? I measured Serpin C1 level in urine.
G. Hernandez
Verified customer
Asked: 2016-08-01
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-08-01
