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Product Info Summary
| SKU: | EK0840 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. |
Product info
Product Name
Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine®
SKU/Catalog Number
EK0840
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine® (96 Tests). Quantitate Human TFPI in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0840)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: D29-K282
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0840 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0840 is reactive to TFPI in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.
Application Guarantee
EK0840 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TFPI
TFPI (Tissue factor pathway inhibitor),also known as EPI, LACI or TFPI1, is a single-chain polypeptide which can reversibly inhibit Factor Xa (Xa) and Thrombin (Factor IIa). The gene for TFPI is located on chromosome 2q31-q32.1, and has nine exons which span 70 kb. TFPI consists of a highly negatively-charged amino-terminus, three tandemly-linked Kunitz domains, and a highly positively-charged carboxy-terminus.TFPI contributes significantly to the inhibition of Xa in vivo, despite being present at concentrations of only 2.5 nM.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0840-CAP | Anti-Human TFPI Pre-coated 96-well strip microplate | 1 |
| EK0840-ST | Human TFPI Standard | 2 vials, 10 ng/tube |
| EK0840-DA | Human TFPI Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.043 | 0.069 | 0.119 | 0.167 | 0.283 | 0.515 | 0.944 | 1.764 |
Data Example Images
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Human TFPI PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TFPI, Dilution ratio of 1:500, concentration in serum and plasma is 10.4-538.8 ng/ml..
Some articles we found to cite concentrations of TFPI in samples: 14610342 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 57 | 238 | 848 | 55 | 237 | 897 |
| Standard deviation | 3.3 | 11.9 | 66.14 | 3.46 | 14.93 | 75.34 |
| CV (%) | 5.8% | 5% | 7.8% | 6.3%% | 6.3% | 8.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TFPI in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 57 | 57 | 66 | 64 | 61 | 4.06 | 6.6% |
| Sample 2 | 238 | 272 | 240 | 245 | 248 | 13.66 | 5.5% |
| Sample 3 | 848 | 982 | 998 | 845 | 918 | 71.98 | 7.8% |
Protein Target Info & Infographic
Gene/Protein Information For TFPI (Source: Uniprot.Org, NCBI)
Gene Name
TFPI
Full Name
Tissue factor pathway inhibitor
Weight
35015 MW
Alternative Names
Tissue factor pathway inhibitor;TFPI;Extrinsic pathway inhibitor;EPI;Lipoprotein-associated coagulation inhibitor;LACI;TFPI;LACI, TFPI1; TFPI EPI, LACI, TFI1, TFPI tissue factor pathway inhibitor tissue factor pathway inhibitor|anti-convertin|extrinsic pathway inhibitor|tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TFPI, check out the TFPI Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TFPI: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine® (EK0840)
Hello CJ!
EK0840 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu Y, Qin X, Zhou J, Tu Z, Bi X, Li W, Fan X, Zhang Y. Oncol Lett. 2011 Sep 1;2(5):779-783. Epub 2011 Jul 5. Tissue Factor Pathway Inhibitor-2 Inhibits The Growth And Invasion Of Hepatocellular Carcinoma Cells And Is Inactivated In Human Hepatocel...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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11 Customer Q&As for Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine®
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
H. Wu
Verified customer
Asked: 2020-12-09
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-12-09
Question
What regions of the TFPI molecule does EK0840 detect?
Verified customer
Asked: 2020-11-30
Answer
The immunogen sequence for both the capture and detection antbodies in the Human TFPI / Tissue Factor Pathway Inhibitor ELISA Kit PicoKine™ (EK0840) is D29-K282 Accession # P10646.
Boster Scientific Support
Answered: 2020-11-30
Question
Q: if the enzyme conjugated TFPI antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TFPI antigen?
Verified Customer
Verified customer
Asked: 2020-11-28
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-28
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
V. Smith
Verified customer
Asked: 2020-07-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
D. Gandhi
Verified customer
Asked: 2020-06-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-06-09
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TFPI using the EK0840 Human TFPI Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-05-08
Question
Q: Can TFPI ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-11-15
Question
Q: can you recommend the dilution ratio of serum samples for detection of TFPI in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Y. Mitchell
Verified customer
Asked: 2019-10-05
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TFPI ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-10-05
Question
Q: which procedure should I follow in order to thaw whole blood sample for TFPI ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TFPI for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-09-06
Question
Q: how to proceed with the analysis of ELISA data? I have obtained TFPI level in plasma.
Verified Customer
Verified customer
Asked: 2018-04-22
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-04-22
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
T. Davis
Verified customer
Asked: 2017-01-05
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-01-05
