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Product Info Summary
| SKU: | A00622 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ATTY/TAT Antibody Picoband®
SKU/Catalog Number
A00622
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATTY/TAT Antibody Picoband® catalog # A00622. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATTY/TAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00622)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ATTY, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00622 is reactive to TAT in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
50399 MW
Background of TAT
This nuclear gene encodes a mitochondrial protein tyrosine aminotransferase which is present in the liver and catalyzes the conversion of L-tyrosine into p-hydroxyphenylpyruvate. Mutations in this gene cause tyrosinemia (type II, Richner-Hanhart syndrome), a disorder accompanied by major skin and corneal lesions, with possible mental retardation. A regulator gene for tyrosine aminotransferase is X-linked.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00622 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: rat spleen tissue, mouse liver tissue, A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ATTY using anti-ATTY antibody (A00622).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: A549 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATTY antigen affinity purified polyclonal antibody (Catalog # A00622) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATTY at approximately 42KD. The expected band size for ATTY is at 50KD.
Protein Target Info & Infographic
Gene/Protein Information For TAT (Source: Uniprot.org, NCBI)
Gene Name
TAT
Full Name
Tyrosine aminotransferase
Weight
50399 MW
Superfamily
class-I pyridoxal-phosphate-dependent aminotransferase family
Alternative Names
Tyrosine aminotransferase;TAT;2.6.1.5;L-tyrosine:2-oxoglutarate aminotransferase;TAT; TAT tyrosine aminotransferase tyrosine aminotransferase|L-tyrosine:2-oxoglutarate aminotransferase|testis tissue sperm-binding protein Li 34a|tyrosine aminotransferase, cytosolic
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TAT, check out the TAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATTY/TAT Antibody Picoband® (A00622)
Hello CJ!
A00622 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142
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Customer Q&As
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1 Customer Q&As for Anti-ATTY/TAT Antibody Picoband®
Question
We are currently using anti-ATTY/TAT antibody A00622 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The anti-ATTY/TAT antibody (A00622) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-24
