Product Info Summary
SKU: | EK0740 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine®
View all CXCL13/BLC/BCA-1 ELISA kits
SKU/Catalog Number
EK0740
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cxcl13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0740)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: I22-A109
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0740 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0740 is reactive to CXCL13 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0740 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL13/BLC/BCA-1
C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK0740-CAP | Anti-Mouse CXCL13 Pre-coated 96-well strip microplate | 1 |
EK0740-ST | Mouse CXCL13 Standard | 2 vials, 10 ng/tube |
EK0740-DA | Mouse CXCL13 Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.028 | 0.039 | 0.059 | 0.090 | 0.191 | 0.469 | 1.058 | 1.927 |
Data Example Images
Click image to see more details
Mouse CXCL13/BLC PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL13/BLC/BCA-1, Dilution ratio of 1:1, concentration in serum and plasma is 0-1155 pg/ml..
Some articles we found to cite concentrations of CXCL13/BLC/BCA-1 in samples: 23936541 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 31 | 182 | 491 | 28 | 184 | 451 |
Standard deviation | 1.39 | 8 | 31.42 | 1.4 | 11.22 | 33.82 |
CV (%) | 4.5% | 4.4% | 6.4% | 5% | 6.1% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CXCL13/BLC/BCA-1 in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 31 | 34 | 32 | 29 | 31 | 1.8 | 5.8% |
Sample 2 | 182 | 204 | 199 | 194 | 194 | 8.16 | 4.2% |
Sample 3 | 491 | 507 | 522 | 441 | 490 | 30.47 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For CXCL13 (Source: Uniprot.Org, NCBI)
Gene Name
CXCL13
Full Name
C-X-C motif chemokine 13
Weight
11927 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA1; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; CXCL13; SCYB13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant) CXCL13 ANGIE, ANGIE2, BCA-1, BCA1, BLC, BLR1L, SCYB13 C-X-C motif chemokine ligand 13 C-X-C motif chemokine 13|B-cell chemoattractant|B-cell-attracting chemokine 1|B-cell-homing chemokine (ligand for Burkitts lymphoma receptor-1)|B-lymphocyte chemoattractant|CXC chemokine BLC|b cell-attracting chemokine 1|b lymphocyte chemoattractant|chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)|small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant)|small-inducible cytokine B13
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CXCL13, check out the CXCL13 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CXCL13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine® (EK0740)
Hello CJ!
EK0740 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A prokaryotic-eukaryotic hybrid viral vector for delivery of large cargos of genes and proteins into human cells
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Customer Q&As
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11 Customer Q&As for Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine®
Question
Does EK0740 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Mouse CXCL13 / BLC / BCA1 ELISA Kit PicoKine® (EK0740) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-10
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
T. Lewis
Verified customer
Asked: 2019-02-11
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-02-11
Question
Q: how to analyze ELISA data? I measured CXCL13 level in serum.
K. Baker
Verified customer
Asked: 2018-12-30
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-12-30
Question
Q: if the enzyme conjugated CXCL13 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CXCL13 antigen?
R. Gonzalez
Verified customer
Asked: 2018-07-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-07-30
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring CXCL13 using the EK0740 Mouse CXCL13 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-07-01
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2018-07-01
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CXCL13 in Mouse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-12-06
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CXCL13 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-12-06
Question
Q: which procedure should I follow in order to thaw whole blood sample for CXCL13 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-12-05
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CXCL13 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2017-12-05
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
A. Carter
Verified customer
Asked: 2017-09-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-09-20
Question
Q: What is the optimal O.D. value for CXCL13 ELISA kit? I used your CXCL13 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CXCL13 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2017-03-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CXCL13 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CXCL13 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2017-03-14
Question
Q: can I use citrate plasma as samples in Mouse CXCL13 Picokine® ELISA Kit (Catalog # EK0740)?
B. Hall
Verified customer
Asked: 2015-03-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CXCL13 causing degradation of its protein structure. CXCL13 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CXCL13 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2015-03-15