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Product Info Summary
| SKU: | A00429-Dyl550 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human TLR1 DyLight® 550 conjugated Antibody
SKU/Catalog Number
A00429-Dyl550
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human TLR1 DyLight® 550 conjugated Antibody catalog # A00429-Dyl550. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human TLR1 DyLight® 550 conjugated Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00429-Dyl550)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human TLR1 recombinant protein (Position: F350-D404).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00429-Dyl550 is reactive to TLR1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
90.291kDa
Background of TLR1
Toll-like receptor 1 (TLR1), also called TIL or CD281 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. This gene is mapped to 4p14 by fluorescence in situ hybridization. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00429-Dyl550 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For TLR1 (Source: Uniprot.org, NCBI)
Gene Name
TLR1
Full Name
Toll-like receptor 1
Weight
90.291kDa
Superfamily
Toll-like receptor family
Alternative Names
Toll-like receptor 1; Toll/interleukin-1 receptor-like protein; TIL; CD281; TLR1; KIAA0012 TLR1 CD281, TIL, TIL. LPRS5, rsc786 toll like receptor 1 toll-like receptor 1|toll/interleukin-1 receptor-like protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR1, check out the TLR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human TLR1 DyLight® 550 conjugated Antibody (A00429-Dyl550)
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-Human TLR1 DyLight® 550 conjugated Antibody
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Human TLR1 DyLight® 550 conjugated antibody A00429-Dyl550. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-18
Question
My question regarding product A00429-Dyl550, anti-Human TLR1 DyLight® 550 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00429-Dyl550 anti-Human TLR1 DyLight® 550 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-28
Question
Does anti-Human TLR1 DyLight® 550 conjugated antibody A00429-Dyl550 work for Flow Cytometry with brain?
A. Edwards
Verified customer
Asked: 2015-12-24
Answer
According to the expression profile of brain, TLR1 is highly expressed in brain. So, it is likely that anti-Human TLR1 DyLight® 550 conjugated antibody A00429-Dyl550 will work for Flow Cytometry with brain.
Boster Scientific Support
Answered: 2015-12-24
Question
Will A00429-Dyl550 anti-Human TLR1 DyLight® 550 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Li
Verified customer
Asked: 2014-04-02
Answer
You can see on the product datasheet, A00429-Dyl550 anti-Human TLR1 DyLight® 550 conjugated antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-04-02
