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Product Info Summary
| SKU: | EK2141 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) |
Product info
Product Name
Mouse BID ELISA Kit PicoKine®
SKU/Catalog Number
EK2141
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse BID ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Bid in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse BID ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2141)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: M1-D195
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2141 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2141 is reactive to Bid in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate)
Application Guarantee
EK2141 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of BID
The BH3 interacting-domain death agonist, or BID, gene is a pro-apoptotic member of the Bcl-2 protein family. This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2, and thus regulate apoptosis. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2141-CAP | Anti-Mouse Bid Pre-coated 96-well strip microplate | 1 |
| EK2141-ST | Mouse Bid Standard | 2 vials, 10 ng/tube |
| EK2141-DA | Mouse Bid Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1,000 | 2,000 |
| O.D. | 0.004 | 0.106 | 0.319 | 0.450 | 0.829 | 1.311 | 1.827 | 2.132 |
Data Example Images
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Mouse BID PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse BID ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 45 | 265 | 1125 | 55 | 266 | 1155 |
| Standard deviation | 2.03 | 14.58 | 67.5 | 3.36 | 13.03 | 64.68 |
| CV(%) | 4.5% | 5.5% | 6% | 6.1% | 4.9% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of BID in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 45 | 49 | 61 | 55 | 53 | 3.05 | 5.8% |
| Sample 2 | 265 | 285 | 290 | 296 | 284 | 11.08 | 3.9% |
| Sample 3 | 1125 | 1325 | 1266 | 1451 | 1292 | 63.3 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For Bid (Source: Uniprot.Org, NCBI)
Gene Name
Bid
Full Name
BH3-interacting domain death agonist
Weight
21.995kDa
Alternative Names
apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID BID FP497 BH3 interacting domain death agonist BH3-interacting domain death agonist|Human BID coding sequence|apoptic death agonist|desmocollin type 4|p22 BID
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Bid, check out the Bid Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Bid: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse BID ELISA Kit PicoKine® (EK2141)
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