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Product Info Summary
| SKU: | EKC1966 |
|---|---|
| Size: | 1 kit, containing two 96-well plates and all necessary reagents |
| Reactive Species: | Human, Mouse, Rat |
| Application: | ELISA |
| Sample Types: | Cell lines |
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Product info
Product Name
IGF1R (Phospho-Tyr1161) Colorimetric Cell-Based ELISA Kit
See all IGF-I R/IGF1R products
SKU/Catalog Number
EKC1966
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The IGF1R (Phospho-Tyr1161) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor IGF1R (Phospho-Tyr1161) protein expression profile in cells. The kit can be used for measuring the relative amounts of IGF1R (Phospho-Tyr1161) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on IGF1R (Phospho-Tyr1161).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
IGF1R (Phospho-Tyr1161) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1966)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1966 is reactive to IGF1R in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1966 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Reagent | Quantity | Container |
|---|---|---|
| 96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
| 10x TBS | 24 ml (10x) | Clear |
| Quenching Buffer | 24 ml (1x) | Clear |
| Blocking Buffer | 50 ml (1x) | Clear |
| 15x Wash Buffer | 50 ml (15x) | Clear |
| 100x Anti-IGF1R Phospho-Tyr1161 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
| 100x Anti-IGF1R Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
| 100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
| HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
| HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
| Primary Antibody Diluent | 12 ml (1x) | Clear |
| Ready-to-Use Substrate | 12 ml (1x) | Brown |
| Stop Solution | 12 ml (1x) | Clear |
| Crystal Violet Solution | 12ml (1x) | Glass |
| SDS Solution | 24 ml (1x) | Clear |
| Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
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Boster Kit Box
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Western blot analysis of extracts from 293 cells treated with Insulin
Protein Target Info & Infographic
Gene/Protein Information For IGF1R (Source: Uniprot.Org, NCBI)
Gene Name
IGF1R
Full Name
Insulin-like growth factor 1 receptor
Weight
154793 MW
Superfamily
protein kinase superfamily
Alternative Names
Insulin-like growth factor 1 receptor;Insulin-like growth factor I receptor;IGF-I receptor;CD221 antigen IGF1R CD221, IGFIR, IGFR, JTK13 insulin like growth factor 1 receptor insulin-like growth factor 1 receptor|IGF-I receptor|soluble IGF1R variant 1|soluble IGF1R variant 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IGF1R, check out the IGF1R Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGF1R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For IGF1R (Phospho-Tyr1161) Colorimetric Cell-Based ELISA Kit (EKC1966)
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