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Product Info Summary
| SKU: | EK0578 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Growth Hormone/GH1 ELISA Kit PicoKine®
View all Growth Hormone ELISA kits
SKU/Catalog Number
EK0578
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Growth Hormone/GH1 ELISA Kit PicoKine® (96 Tests). Quantitate Human GH1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Growth Hormone/GH1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0578)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: F27-F217
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0578 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0578 is reactive to GH1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0578 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Growth Hormone
Growth hormone (GH) is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. The human genes for growth hormone (GH), has been located on chromosome 17 in humans. GH replacement of adults with acquired GH deficiency (GHD) results in body composition changes including increases in lean mass and bone mineral density. However, the effects of long-term GH therapy on cognitive function are largely unknown, and there are conflicting data regarding quality of life. The standard product used in this kit is recombinant human Growth Hormone, consisting of 192 amino acids with the molecular mass of 22KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0578-CAP | Anti-Human GH1 Pre-coated 96-well strip microplate | 1 |
| EK0578-ST | Human GH1 Standard | 2 vials, 10 ng/tube |
| EK0578-DA | Human GH1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.042 | 0.129 | 0.229 | 0.378 | 0.609 | 1.024 | 1.347 | 2.098 |
Data Example Images
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Human Growth Hormone PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Growth Hormone, Dilution ratio of 1:1, concentration in serum and plasma is around 200 pg/ml..
Some articles we found to cite concentrations of Growth Hormone in samples: 2227604 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Growth Hormone/GH1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 29 | 192 | 394 | 28 | 188 | 423 |
| Standard deviation | 2.2 | 11.13 | 30.33 | 2.29 | 11.84 | 39.76 |
| CV (%) | 7.6% | 5.8% | 7.7% | 8.2% | 6.3% | 9.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Growth Hormone in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 29 | 31 | 29 | 29 | 0.86 | 2.9% |
| Sample 2 | 192 | 199 | 200 | 197 | 197 | 3.08 | 1.5% |
| Sample 3 | 394 | 427 | 443 | 440 | 426 | 19.42 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For GH1 (Source: Uniprot.Org, NCBI)
Gene Name
GH1
Full Name
Somatotropin
Weight
24847 MW
Superfamily
somatotropin/prolactin family
Alternative Names
Growth hormone 1 isoform 1 ;HCG1749481, isoform CRA_s ;GH1 ;hCG_1749481 ; GH1 GH, GH-N, GHB5, GHN, IGHD1A, IGHD1B, IGHD2, hGH-N growth hormone 1 somatotropin|growth hormone B5|pituitary growth hormone
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on GH1, check out the GH1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for GH1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Growth Hormone/GH1 ELISA Kit PicoKine® (EK0578)
Hello CJ!
EK0578 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inorganic Nitrate Mimics Exercise-Stimulated Muscular Fiber-Type Switching and Myokine and γ-Aminobutyric Acid Release
Clinical efficacy and mechanism of growth hormone action in patients experiencing repeat implantation failure
Alashti, M.,Shirvani, H.,Sabzevari Rad,R. et al.Effects of different small-sided games on blood lactate and GH/IGF-1 axis responses in young soccer players.Ger J Exerc Sport Res(2020).https://doi.org/10.1007/s1266 2-020-00691-6
Species: EK0578;APP:ELISA;SAMPLE:BLOOD;DILUTION:NA|EK0776;APP:ELISA;SAMPLE:BLOOD;DILUTION:NA
Inorganic nitrate mimics exercise-stimulated muscular fiber-type switching and myokine and %u03B3-aminobutyric acid release
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Human Growth Hormone/GH1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Lewis
Verified customer
Asked: 2020-07-16
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-16
Question
Q: Can Growth Hormone ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-06-20
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-06-20
Question
Q: is it okay to use citrate plasma as samples in Human Growth Hormone Picokine® ELISA Kit (Catalog # EK0578)?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Growth Hormone causing degradation of its protein structure. Growth Hormone may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Growth Hormone ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-09-17
Question
Q: What is the optimal O.D. value for Growth Hormone ELISA kit? I used your Growth Hormone ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Growth Hormone even though the O.D. values are not very high?
L. Martinez
Verified customer
Asked: 2019-01-04
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Growth Hormone ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of Growth Hormone that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2019-01-04
