Anti-I-309/CCL1 Antibody Picoband®

CCL1/I-309/TCA-3 antibody

Boster Bio Anti-I-309/CCL1 Antibody Picoband® catalog # PB9028. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 4 publication(s).

Product Info Summary

SKU: PB9028
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, WB

Product Name

Anti-I-309/CCL1 Antibody Picoband®

View all CCL1/I-309/TCA-3 Antibodies

SKU/Catalog Number

PB9028

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-I-309/CCL1 Antibody Picoband® catalog # PB9028. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-I-309/CCL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9028)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human I-309 recombinant protein (Position: K24-K96). Human I-309 shares 38% amino acid (aa) sequence identity with mouse I-309.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9028 is reactive to CCL1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

11 kDa

Calculated molecular weight

154793 MW

Background of CCL1/I-309/TCA-3

CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PB9028 is guaranteed for ELISA, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -

Positive Control

WB: U87 Whole Cell, MCF-7 Whole Cell, COLO320 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For CCL1 (Source: Uniprot.org, NCBI)

Gene Name

CCL1

Full Name

C-C motif chemokine 1

Weight

154793 MW

Superfamily

intercrine beta (chemokine CC) family

Alternative Names

Insulin-like growth factor 1 receptor;2.7.10.1;Insulin-like growth factor I receptor;IGF-I receptor;CD221;Insulin-like growth factor 1 receptor alpha chain;Insulin-like growth factor 1 receptor beta chain;IGF1R; CCL1 I-309, P500, SCYA1, SISe, TCA3 C-C motif chemokine ligand 1 C-C motif chemokine 1|T lymphocyte-secreted protein I-309|chemokine (C-C motif) ligand 1|inflammatory cytokine I-309|small inducible cytokine A1 (I-309, homologous to mouse Tca-3)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCL1, check out the CCL1 Infographic

CCL1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9028 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Long-pulse gastric electrical stimulation protects interstitial cells of Cajal in diabetic rats via IGF-1 signaling pathway

Insulin-like growth factors in endometrioid adenocarcinoma: Correlation with clinico-pathological features and estrogen receptor expression

Treating Human Meniscal Fibrochondrocytes with hIGF-1 Gene by Liposome

Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression

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6 Customer Q&As for Anti-I-309/CCL1 Antibody Picoband®

Question

We are currently using anti-I-309/CCL1 antibody PB9028 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

P. Baker

Verified customer

Asked: 2020-02-10

Answer

The anti-I-309/CCL1 antibody (PB9028) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-10

Question

Do you have a BSA free version of anti-I-309/CCL1 antibody PB9028 available?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-I-309/CCL1 antibody PB9028 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-08

Question

Here is the WB image, lot number and protocol we used for brain using anti-I-309/CCL1 antibody PB9028. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-08

Question

I see that the anti-I-309/CCL1 antibody PB9028 works with ELISA, what is the protocol used to produce the result images on the product page?

J. Baker

Verified customer

Asked: 2019-05-30

Answer

You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-30

Question

I have a question about product PB9028, anti-I-309/CCL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Carter

Verified customer

Asked: 2018-11-26

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9028 anti-I-309/CCL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-11-26

Question

I was wanting to use your anti-I-309/CCL1 antibody for ELISA for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?

Verified Customer

Verified customer

Asked: 2017-07-19

Answer

You can see on the product datasheet, PB9028 anti-I-309/CCL1 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-07-19

Order DetailsPrice
PB9028

100μg

$370
PB9028-10ug

10μg sample (liquid)

$99
PB9028-Biotin

100 μg Biotin conjugated

$570
PB9028-Cy3

100 μg Cy3 conjugated

$570
PB9028-Dylight488

100 μg Dylight488 conjugated

$570
PB9028-Dylight550

100 μg Dylight550 conjugated

$570
PB9028-Dylight594

100 μg Dylight594 conjugated

$570
PB9028-FITC

100 μg FITC conjugated

$570
PB9028-HRP

100 μg HRP conjugated

$570
PB9028-APC

100 μg APC conjugated

$670
PB9028-PE

100 μg PE conjugated

$670
PB9028-iFluor647

100 μg iFluor647 conjugated

$670
PB9028-carrier-free

Carrier Free

$370

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PB9028
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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