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Product Info Summary
| SKU: | PA1826 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Myoglobin/MB Antibody Picoband®
SKU/Catalog Number
PA1826
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Myoglobin/MB Antibody catalog # PA1826. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Myoglobin/MB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1826)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Myoglobin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1826 is reactive to MB in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
17184 MW
Background of Myoglobin
Myoglobin (MB) also known as PVALB, is a single-chain globular protein of 153 or 154 amino acids, containing a heme (iron-containing porphyrin) prosthetic group in the center around which the remaining apoprotein folds. It is a member of the globin superfamily and is expressed in skeletal and cardiac muscles. This gene is mapped to chromosome 22q11-q13. Myoglobin is released from damaged muscle tissue (rhabdomyolysis), which has very high concentrations of myoglobin. The released myoglobin is filtered by the kidneys but is toxic to the renal tubular epithelium and so may cause acute renal failure.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1826 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue, Human Placenta Tissue,
Validation Images & Assay Conditions
Click image to see more details
Anti-Myoglobin antibody, PA1826, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: Human Placenta Tissue Lysate
Protein Target Info & Infographic
Gene/Protein Information For MB (Source: Uniprot.org, NCBI)
Gene Name
MB
Full Name
Myoglobin
Weight
17184 MW
Superfamily
globin family
Alternative Names
Myoglobin;MB; MB PVALB myoglobin myoglobin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MB, check out the MB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Myoglobin/MB Antibody Picoband® (PA1826)
Hello CJ!
PA1826 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Peng L, Yanjiao M, Ai-Guo W, Pengtao G, Jianhua L, Ju Y, Hongsheng O, Xichen Z. Biochem Biophys Res Commun. 2011 Jun 3;409(2):344-9. Doi: 10.1016/J.Bbrc.2011.05.021. Epub 2011 May 8. A Fine Balance Between Ccnl1 And Timp1 Contributes To The Develo...
Xu H, He J, Zhang Y, Fan L, Zhao Y, Xu T, Nie Z, Li X, Huang Z, Lu B, Xu P. Carbohydr Polym. 2015 May 5;121:132-9. Doi: 10.1016/J.Carbpol.2014.12.057. Epub 2015 Jan 3. Synthesis And In Vitro Evaluation Of A Hyaluronic Acid-Quantum Dots-Melphalan C...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Myoglobin/MB Antibody Picoband®
Question
See attached the WB image, lot number and protocol we used for heart using anti-Myoglobin/MB antibody PA1826. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-20
Question
We have observed staining in human skeletal muscle. What should we do? Is anti-Myoglobin/MB antibody supposed to stain skeletal muscle positively?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
From literature skeletal muscle does express MB. From Uniprot.org, MB is expressed in heart right ventricle, skeletal muscle, heart, among other tissues. Regarding which tissues have MB expression, here are a few articles citing expression in various tissues:
Heart, Pubmed ID: 7895732
Skeletal muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-02
Question
Is this PA1826 anti-Myoglobin/MB antibody reactive to the isotypes of MB?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The immunogen of PA1826 anti-Myoglobin/MB antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Myoglobin(138-154aa LFRKDMASNYKELGFQG). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-21
Question
I have a question about product PA1826, anti-Myoglobin/MB antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1826 anti-Myoglobin/MB antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-12
Question
I am interested in to test anti-Myoglobin/MB antibody PA1826 on human heart for research purposes, then I may be interested in using anti-Myoglobin/MB antibody PA1826 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-22
Answer
The products we sell, including anti-Myoglobin/MB antibody PA1826, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-22
Question
Our lab want to know about using your anti-Myoglobin/MB antibody for brown fat cell differentiation studies. Has this antibody been tested with western blotting on tissue lysate? We would like to see some validation images before ordering.
O. Krishna
Verified customer
Asked: 2019-08-05
Answer
We appreciate your inquiry. This PA1826 anti-Myoglobin/MB antibody is tested on human placenta tissue, tissue lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-05
Question
Would anti-Myoglobin/MB antibody PA1826 work on monkey WB with skeletal muscle?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
Our lab technicians have not tested anti-Myoglobin/MB antibody PA1826 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Myoglobin/MB antibody PA1826 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey skeletal muscle in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-19
Question
Would anti-Myoglobin/MB antibody PA1826 work for WB with heart?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
According to the expression profile of heart, MB is highly expressed in heart. So, it is likely that anti-Myoglobin/MB antibody PA1826 will work for WB with heart.
Boster Scientific Support
Answered: 2019-07-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Myoglobin/MB antibody PA1826. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-20
Question
I see that the anti-Myoglobin/MB antibody PA1826 works with WB, what is the protocol used to produce the result images on the product page?
Z. Mitchell
Verified customer
Asked: 2018-10-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-10-18
Question
We are currently using anti-Myoglobin/MB antibody PA1826 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
The anti-Myoglobin/MB antibody (PA1826) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-17
Question
Is a blocking peptide available for product anti-Myoglobin/MB antibody (PA1826)?
E. Patel
Verified customer
Asked: 2018-03-30
Answer
We do provide the blocking peptide for product anti-Myoglobin/MB antibody (PA1826). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-30
Question
Is there a BSA free version of anti-Myoglobin/MB antibody PA1826 available?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Myoglobin/MB antibody PA1826 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-29
Question
I was wanting to use your anti-Myoglobin/MB antibody for WB for human heart on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human heart identification?
D. Zhao
Verified customer
Asked: 2017-08-09
Answer
You can see on the product datasheet, PA1826 anti-Myoglobin/MB antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-09
Question
Would PA1826 anti-Myoglobin/MB antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
E. Zhang
Verified customer
Asked: 2017-01-06
Answer
As indicated on the product datasheet, PA1826 anti-Myoglobin/MB antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-06
