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Product Info Summary
| SKU: | PB9345 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband®
View all t-Plasminogen Activator/tPA Antibodies
SKU/Catalog Number
PB9345
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband® catalog # PB9345. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9345)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TPA recombinant protein (Position: H366-P562). Human TPA shares 83% and 84% amino acid (aa) sequence identity with mouse and rat TPA, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9345 is reactive to PLAT in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
63-69 kDa
Calculated molecular weight
62917 MW
Background of t-Plasminogen Activator/tPA
PLAT is also known as tPA. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9345 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human PC-3 whole cell, human U87 whole cell, human SH-SY5Y whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PLAT using anti-PLAT antibody (PB9345).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human PC-3 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLAT antigen affinity purified polyclonal antibody (Catalog # PB9345) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLAT at approximately 63-69 kDa. The expected band size for PLAT is at 63 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PLAT (Source: Uniprot.org, NCBI)
Gene Name
PLAT
Full Name
Tissue-type plasminogen activator
Weight
62917 MW
Superfamily
peptidase S1 family
Alternative Names
Tissue-type plasminogen activator;t-PA;t-plasminogen activator;tPA;3.4.21.68;Alteplase;Reteplase;Tissue-type plasminogen activator chain A;Tissue-type plasminogen activator chain B;PLAT; PLAT T-PA, TPA plasminogen activator, tissue type tissue-type plasminogen activator|alteplase|plasminogen/activator kringle|reteplase|t-plasminogen activator
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PLAT, check out the PLAT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PLAT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband® (PB9345)
Hello CJ!
PB9345 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang J,Li Y,Pan L,Li J,Yu Y,Liu B,Zubair M,Wei Y,Pillay B,Olaniran AO,Chiliza TE,Shao G,Feng Z,Xiong Q.Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) moonlights as an adhesin in Mycoplasma hyorhinis adhesion to epithelial cells as well as a plasminogen receptor mediating extracellular matrix degradation.Vet Res.2021 Jun 3;52(1):80.doi:10.1186/s13567-021-00952-8. PMID:34082810;PMCID:PMC8173509.
Species: Human
PB9345 usage in article: APP:WB, SAMPLE:M. HYORHINIS CELL, DILUTION:1:2000
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Customer Q&As
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1 Customer Q&As for Anti-TPA Tissue Plasminogen Activator/PLAT Antibody Picoband®
Question
We are currently using anti-TPA Tissue Plasminogen Activator/PLAT antibody PB9345 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-06
Answer
The anti-TPA Tissue Plasminogen Activator/PLAT antibody (PB9345) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-06
