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Product Info Summary
| SKU: | PA1348 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Annexin A2/ANXA2 Antibody Picoband®
View all Annexin A2 Antibodies
SKU/Catalog Number
PA1348
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Annexin A2/ANXA2 Antibody catalog # PA1348. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Annexin A2/ANXA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1348)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Annexin A2, different from the rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1348 is reactive to ANXA2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
36 kDa
Calculated molecular weight
38604 MW
Background of Annexin A2
Annexin A2 also known as annexin II is a protein that in humans is encoded by the ANXA2 gene. The ANXA2 gene has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. This protein is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. Richard et al. (1994) presented an integration of the physical, expression, and genetic maps of human chromosome 15. They placed the ANXA2 gene in their region IV, i.e., 15q21-q22, thus confirming the previous localization.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1348 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat, -
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Testis Tissue, Rat Lung Tissue, Rat Ovary Tissue, MCF-7 Cell, SMMC Cell, A549 Cell, JURKAT Cell, mouse spleen tissue, mouse NIH/3T3 whole cell
IHC: Human Intestinal Cancer Tissue
Validation Images & Assay Conditions
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Anti-Annexin A2 antibody, PA1348, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Lung Tissue Lysate
Lane 3: Rat Ovary Tissue Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: SMMC Cell Lysate
Lane 6: A549 Cell Lysate
Lane 7: JURKAT Cell Lysate
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Anti-Annexin A2 antibody, PA1348, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Figure 3. Western blot analysis of Annexin A2 using anti-Annexin A2 antibody (PA1348).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates
Lane 2: mouse NIH/3T3 whole cell lysates
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin A2 antigen affinity purified polyclonal antibody (Catalog # PA1348) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin A2 at approximately 36KD. The expected band size for Annexin A2 is at 39KD.
Protein Target Info & Infographic
Gene/Protein Information For ANXA2 (Source: Uniprot.org, NCBI)
Gene Name
ANXA2
Full Name
Annexin A2
Weight
38604 MW
Superfamily
annexin family
Alternative Names
Annexin A2;Annexin II;Annexin-2;Calpactin I heavy chain;Calpactin-1 heavy chain;Chromobindin-8;Lipocortin II;Placental anticoagulant protein IV;PAP-IV;Protein I;p36;ANXA2;ANX2, ANX2L4, CAL1H, LPC2D; ANXA2 ANX2, ANX2L4, CAL1H, HEL-S-270, LIP2, LPC2, LPC2D, P36, PAP-IV annexin A2 annexin A2|annexin II|annexin-2|calpactin I heavy chain|calpactin I heavy polypeptide|calpactin-1 heavy chain|chromobindin 8|epididymis secretory protein Li 270|epididymis secretory sperm binding protein|lipocortin II|placental anticoagulant protein IV|protein I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ANXA2, check out the ANXA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANXA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Annexin A2/ANXA2 Antibody Picoband® (PA1348)
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Customer Q&As
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3 Customer Q&As for Anti-Annexin A2/ANXA2 Antibody Picoband®
Question
My question regarding product PA1348, anti-Annexin A2/ANXA2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-10
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1348 anti-Annexin A2/ANXA2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-10
Question
We are currently using anti-Annexin A2/ANXA2 antibody PA1348 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
O. Wu
Verified customer
Asked: 2019-04-02
Answer
The anti-Annexin A2/ANXA2 antibody (PA1348) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-02
Question
Would anti-Annexin A2/ANXA2 antibody PA1348 work on monkey IHC with umbilical vein endothelial cell?
Verified Customer
Verified customer
Asked: 2018-11-21
Answer
Our lab technicians have not validated anti-Annexin A2/ANXA2 antibody PA1348 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Annexin A2/ANXA2 antibody PA1348 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey umbilical vein endothelial cell in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-21
