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Product Info Summary
| SKU: | EK1886 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. |
Product info
Product Name
Rat SerpinE2 ELISA Kit PicoKine®
View all Serpin E2/PN1 ELISA kits
SKU/Catalog Number
EK1886
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat SerpinE2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Serpine2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine. Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat SerpinE2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1886)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S20-P397
Sensitivity
<0.1 ng/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK1886 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1886 is reactive to Serpine2 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate) ,urine.
Application Guarantee
EK1886 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin E2/PN1
Glia-derived nexin is a protein that in humans is encoded by the SERPINE2 gene. This gene is mapped to 9q34. SERPINE2 produced in Sf9 Baculovirus cells is a single,glycosylated polypeptide chain containing 386 aminoacids (20-397a.a.) andhaving a molecular mass of 42.9kDa (Molecular size on SDS-PAGE will appear atapproximately 40-57kDa).SERPINE2 is expressed with a 8 aminoacid His tag atC-Terminus and purified by proprietary chromatographic techniques. This gene displays neurite promoting activity and serine protease inhibitory activity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1886-CAP | Anti-Rat Serpine2 Pre-coated 96-well strip microplate | 1 |
| EK1886-ST | Rat Serpine2 Standard | 2 vials, 50 ng/tube |
| EK1886-DA | Rat Serpine2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.089 | 0.125 | 0.181 | 0.287 | 0.449 | 0.886 | 1.85 | 2.043 |
Data Example Images
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Rat SerpinE2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin E2/PN1, Dilution ratio of 1:50, concentration in serum and plasma is 200-500 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat SerpinE2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 413 | 2922 | 9164 | 595 | 3000 | 9451 |
| Standard deviation | 17.35 | 131.49 | 650.64 | 38.68 | 165.00 | 737.18 |
| CV (%) | 4.2% | 4.5% | 7.1% | 6.5% | 5.5% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin E2/PN1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 413 | 438 | 416 | 463 | 432 | 20.08 | 4.6% |
| Sample 2 | 2922 | 2913 | 2827 | 3068 | 2932 | 86.57 | 2.9% |
| Sample 3 | 9164 | 8948 | 9570 | 8836 | 9129 | 280.31 | 3.0% |
Protein Target Info & Infographic
Gene/Protein Information For Serpine2 (Source: Uniprot.Org, NCBI)
Gene Name
Serpine2
Full Name
Glia-derived nexin
Weight
44.002kDa
Superfamily
serpin family
Alternative Names
Glia-derived nexin; GDN; Peptidase inhibitor 7; PI-7; Protease nexin 1; PN-1; Protease nexin I; Serpin E2; Serpine2; Pi7; Pn1 SERPINE2 GDN, GDNPF, PI-7, PI7, PN-1, PN1, PNI serpin family E member 2 glia-derived nexin|glial-derived neurite promoting factor|peptidase inhibitor 7|protease nexin I|serpin E2|serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Serpine2, check out the Serpine2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Serpine2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat SerpinE2 ELISA Kit PicoKine® (EK1886)
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Customer Q&As
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10 Customer Q&As for Rat SerpinE2 ELISA Kit PicoKine®
Question
Q: is it okay to use heparin plasma as samples in Rat Serpin E2 Picokine® ELISA Kit (Catalog # EK1886)?
Verified Customer
Verified customer
Asked: 2020-12-08
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Serpin E2 causing disruption of its protein structure. Serpin E2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Serpin E2 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-12-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Zhou
Verified customer
Asked: 2020-08-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-12
Question
Q: if the enzyme conjugated Serpin E2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin E2 antigen?
G. Zhou
Verified customer
Asked: 2020-07-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-07-17
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Serpin E2 in Rat plasma,urine? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-06-17
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Serpin E2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-06-17
Question
Q: which procedure should I follow in order to thaw whole blood sample for Serpin E2 ELISA after freezing?
G. Kapoor
Verified customer
Asked: 2019-11-12
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin E2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-11-12
Question
Q: What is the optimal O.D. value for Serpin E2 ELISA kit? I performed your Serpin E2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Serpin E2 even though the O.D. values are not very high?
T. Bhasin
Verified customer
Asked: 2019-09-12
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin E2 ELISA kit to have sensitivity of 0.1ng/ml, that means the minimum amount of Serpin E2 that can be declared/interpreted as positive by the above standard is 0.1ng/ml.
Boster Scientific Support
Answered: 2019-09-12
Question
Q: how to analyze ELISA data? I have obtained Serpin E2 level in plasma,urine.
Verified Customer
Verified customer
Asked: 2019-03-19
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-03-19
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-01-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-01-25
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-15
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Serpin E2 using the EK1886 Rat Serpin E2 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-02-14
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2016-02-14
