Product Info Summary
SKU: | EZ0928 |
---|---|
Size: | For 5 plates, 96 wells each |
Reactive Species: | Human |
Application: | ELISA |
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Product info
Product Name
Human Tissue Factor/F3 ELISA Kit EZ-Set™ (DIY Antibody Pairs)
See all Coagulation Factor III/Tissue Factor products
SKU/Catalog Number
EZ0928
Size
For 5 plates, 96 wells each
*Question: How many samples can I assay/run in this kit?
Description
Human Tissue Factor/F3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Tissue Factor/F3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0928)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: S33-E251
Assay Range
15.6 pg/ml - 1,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EZ0928 is reactive to F3 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) , Cell lysates, and urine.
Application Guarantee
EZ0928 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Catalog number | Description | Quantity | Storage of opened/reconstituted material |
---|---|---|---|
EZ0928-CA | Rabbit anti- human F3 polyclonal antibody (Capture Antibody) | 500 µl, 0.4 mg/mL (recommended dilution 1:100) | Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. |
EZ0928-DA | Biotinylated goat anti- human F3 polyclonal antibody (Detection Antibody) | 500 µl, 5 µg/mL (recommended dilution 1:100) | |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 500 µl (recommended dilution 1:100) | |
EK0928-ST | Lyophilized recombinant human F3 standard | 10 ng/tube×3 | Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- 1. Microplate reader in standard size.
- 2. Automated plate washer.
- 3. Incubator.
- 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- 5. Clean tubes and Eppendorf tubes.
- 6. 96 well microplate (Cat# AR1100)
- 7. Plate Sealers.
- 8. Capture Antibody Diluent: PBS.
- 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
- 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
- 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
- 12. Wash Buffer (PBS and PBS-T). Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. - *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.035 | 0.09 | 0.155 | 0.254 | 0.479 | 0.914 | 1.446 | 2.308 |
Data Example Images
![ez0928 2 ELISA human tissue factor f3 ez set elisa kit diy antibody pairs ez0928 2 ELISA human tissue factor f3 ez set elisa kit diy antibody pairs](https://www.bosterbio.com/media/catalog/product/antibody/ez0928-2-ELISA-human-tissue-factor-f3-ez-set-elisa-kit-diy-antibody-pairs.jpg)
Click image to see more details
Human Tissue factor/F3 EZ Set ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 106 | 285 | 593 | 162 | 321 | 630 |
Standard deviation | 3.71 | 15.39 | 29.06 | 9.07 | 22.15 | 36.54 |
CV (%) | 3.5% | 5.4% | 4.9% | 5.6% | 6.9% | 5.8% |
Protein Target Info & Infographic
Gene/Protein Information For F3 (Source: Uniprot.Org, NCBI)
Gene Name
F3
Full Name
Tissue factor
Weight
33068 MW
Superfamily
tissue factor family
Alternative Names
CD142 antigen; CD142; coagulation factor III (thromboplastin, tissue factor); Coagulation Factor III; F3; FLJ17960; TF; TFA; Thromboplastin; Tissue Factor F3 CD142, TF, TFA coagulation factor III, tissue factor tissue factor|coagulation factor III (thromboplastin, tissue factor)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on F3, check out the F3 Infographic
![F3 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
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