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Product Info Summary
| SKU: | EK1367 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and cell lysates. |
Product info
Product Name
Mouse Tissue Factor/F3 ELISA Kit PicoKine®
View all Coagulation Factor III/Tissue Factor ELISA kits
SKU/Catalog Number
EK1367
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Tissue Factor/F3 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse F3 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and cell lysates. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Tissue Factor/F3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1367)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A29-E251
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1367 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1367 is reactive to F3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and cell lysates.
Application Guarantee
EK1367 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Coagulation Factor III/Tissue Factor
Tissue factor, also called platelet tissue factor, factor III, thrombokinase, or CD142 is a protein present in sub endothelial tissue and leukocytes necessary for the initiation of thrombin formation from the zymogene prothrombin. Tissue factor gene was mapped to 1pter-p21 by study of somatic cell hybrids with a species-specific sensitive chromogenic assay. This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1367-CAP | Anti-Mouse F3 Pre-coated 96-well strip microplate | 1 |
| EK1367-ST | Mouse F3 Standard | 2 vials, 10 ng/tube |
| EK1367-DA | Mouse F3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.041 | 0.092 | 0.171 | 0.252 | 0.396 | 0.707 | 1.105 | 1.849 |
Data Example Images
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Mouse Tissue Factor/F3 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Tissue Factor/F3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 31 | 167 | 444 | 32 | 169 | 432 |
| Standard deviation | 1.3 | 9.18 | 32.41 | 1.5 | 10.47 | 35.85 |
| CV (%) | 4.2% | 5.5% | 7.3% | 4.7% | 6.2% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Coagulation Factor III/Tissue Factor in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 31 | 36 | 32 | 30 | 32 | 2.27 | 7% |
| Sample 2 | 167 | 171 | 178 | 166 | 170 | 4.71 | 2.7% |
| Sample 3 | 444 | 389 | 387 | 433 | 413 | 25.55 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For F3 (Source: Uniprot.Org, NCBI)
Gene Name
F3
Full Name
Tissue factor
Weight
32935 MW
Superfamily
tissue factor family
Alternative Names
Tissue factor;TF;Coagulation factor III;CD142;F3;Cf-3, Cf3; F3 CD142, TF, TFA coagulation factor III, tissue factor tissue factor|coagulation factor III (thromboplastin, tissue factor)
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on F3, check out the F3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Tissue Factor/F3 ELISA Kit PicoKine® (EK1367)
Hello CJ!
EK1367 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Coagulopathy signature precedes and predicts severity of end‐organ heat stroke pathology in a mouse model
Chen J, Wei D, Zhuang H, Qiao Y, Tang B, Zhang X, Wei J, Fang S, Chen G, Du P, Huang X, Jiang W, Hu Q, Hua Zc. Mol Cell Proteomics. 2011 Jun;10(6):M111.009399. Doi: 10.1074/Mcp.M111.009399. Epub 2011 Apr 7. Proteomic Screening Of Anaerobically Reg...
Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Mouse Tissue Factor/F3 ELISA Kit PicoKine®
Question
Does EK1367 contain recombinant protein? If so, what is the expression system of the recombinant proteins?
Verified customer
Asked: 2020-12-29
Answer
The standards in the Mouse Tissue Factor/F3 ELISA Kit PicoKine™ (EK1367) are recombinant proteins. The expression system is NSO.
Boster Scientific Support
Answered: 2020-12-29
Question
Does EK1367 contain recombinant proteins expressed in insect cells? Do you use the baculovirus vector to transfect target gene to insect cells? If you didn't use the baculovirus vector, could you tell us the insect cell line you used and the method how you transfect target gene to insect cells?
Verified customer
Asked: 2020-12-29
Answer
The Mouse Tissue Factor/F3 ELISA Kit PicoKine™ (EK1367) doesn't contain recombinant proteins expressed in insect cells.
Boster Scientific Support
Answered: 2020-12-29
Question
Are human/primate-origin components included in the manufacturing process of EK1367? If so, what are the human/primate-origin components?
Verified customer
Asked: 2020-12-29
Answer
There wasn't any human/primate-origin components involved in the manufacturing process of the Mouse Tissue Factor/F3 ELISA Kit PicoKine™ (EK1367).
Boster Scientific Support
Answered: 2020-12-29
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-12-12
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-12-12
Question
Q: is it okay to use EDTA plasma as samples in Mouse Tissue Factor Picokine® ELISA Kit (Catalog # EK1367)?
S. Gandhi
Verified customer
Asked: 2019-07-29
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Tissue Factor causing disruption of its protein structure. Tissue Factor may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Tissue Factor ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-07-29
Question
Q: can you recommend the dilution ratio of serum samples for detection of Tissue Factor in Mouse cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Tissue Factor ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-05-06
Question
Q: if the enzyme conjugated Tissue Factor antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Tissue Factor antigen?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-07-17
Question
Q: how to thaw whole blood sample for Tissue Factor ELISA after freezing?
A. Surve
Verified customer
Asked: 2018-05-11
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Tissue Factor for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-05-11
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2015-01-31
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-01-31
