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Product Info Summary
| SKU: | EK1951 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human Renalase ELISA Kit PicoKine®
SKU/Catalog Number
EK1951
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Renalase ELISA Kit PicoKine® (96 Tests). Quantitate Human RNLS in cell culture supernatants, serum and plasma (heparin). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Renalase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1951)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A18-I342
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1951 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1951 is reactive to RNLS in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1951 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Renalase
Renalase, FAD-dependent amine oxidase is an enzyme that in humans is encoded by the RNLS gene. It is mapped to 10q23.31. Renalase is a flavin adenine dinucleotide-dependent amine oxidase that is secreted into the blood from the kidney. The renalase protein consists of a putative secretory signal peptide (SignalP score of 0.4), a flavin adenine dinucleotide (FAD)-binding region, and an oxidase domain. At least four alternative splicing isoforms have been identified in humans (hRenalase1 to hRenalase4). Only hRenalase1 is detected in human blood samples, which means that hRenalase2 to 4 probably have different functions than hRenalase1.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1951-CAP | Anti-Human RNLS Pre-coated 96-well strip microplate | 1 |
| EK1951-ST | Human RNLS Standard | 2 vials, 10 ng/tube |
| EK1951-DA | Human RNLS Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.075 | 0.160 | 0.205 | 0.312 | 0.556 | 0.935 | 1.497 | 2.272 |
Data Example Images
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Human Renalase PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Renalase, Dilution ratio of 1:1, concentration in serum and plasma is 1-2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Renalase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 181 | 1100 | 3799 | 201 | 1115 | 3899 |
| Standard deviation | 7.42 | 47.30 | 174.75 | 11.66 | 76.94 | 280.73 |
| CV (%) | 4.1% | 4.3% | 4.6% | 5.8% | 6.9% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Renalase in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 181 | 171 | 175 | 177 | 176 | 3.60 | 2.0% |
| Sample 2 | 1100 | 1074 | 1106 | 1218 | 1124 | 55.30 | 4.9% |
| Sample 3 | 3799 | 3638 | 3667 | 3825 | 3732 | 80.93 | 2.1% |
Protein Target Info & Infographic
Gene/Protein Information For RNLS (Source: Uniprot.Org, NCBI)
Gene Name
RNLS
Full Name
Renalase
Weight
37.847kDa
Superfamily
renalase family
Alternative Names
Renalase; Monoamine oxidase-C; MAO-C; RNLS; C10orf59 RNLS C10orf59, RENALASE renalase, FAD dependent amine oxidase renalase|MAO-C|alpha-NAD(P)H oxidase/anomerase|monoamine oxidase-C
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RNLS, check out the RNLS Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RNLS: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Renalase ELISA Kit PicoKine® (EK1951)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human Renalase ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I measured Renalase level in plasma.
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-02-18
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Renalase using the EK1951 Human Renalase Picokine® ELISA Kit?
W. Williams
Verified customer
Asked: 2019-08-25
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-08-02
Question
Q: Can Renalase ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-05-30
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-30
Question
Q: can you recommend the dilution ratio of serum samples for detection of Renalase in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
S. Roberts
Verified customer
Asked: 2018-11-25
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Renalase ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-25
Question
Q: which procedure should I follow in order to thaw whole blood sample for Renalase ELISA after freezing?
B. Jones
Verified customer
Asked: 2018-10-12
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Renalase for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-10-12
