Key=store_id Key=entity_id, value=47297 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A00804-1 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2018-04-25 10:29:04 Key=updated_at, value=2024-10-25 05:02:38 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-Oncostatin M/Osm Antibody Picoband® Key=cj_search_index Key=publication_count, value=1 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1199 Key=yoast_content_score, value=30 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=Oncostatin M/Osm Antibody A00804-1 Key=meta_description, value=Boster Bio Anti-Oncostatin M/Osm Antibody Picoband® catalog # A00804-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody. Key=image, value=/a/0/a00804-1-oncostatin-m-primary-antibodies-wb-testing-1.jpg Key=small_image, value=/a/0/a00804-1-oncostatin-m-primary-antibodies-wb-testing-1.jpg Key=thumbnail, value=/a/0/a00804-1-oncostatin-m-primary-antibodies-wb-testing-1.jpg Key=gallery, value=/A00237_1.jpg:/A00237_2.jpg:/A00237_3.jpg Key=url_key, value=anti-oncostatin-m-picoband-trade-antibody-a00804-1-boster Key=url_path, value=anti-oncostatin-m-picoband-trade-antibody-a00804-1-boster Key=options_container, value=Product Info Column Key=image_label, value=Figure 1. Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (A00804-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded wit Key=small_image_label, value=Figure 1. Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (A00804-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded wit Key=thumbnail_label, value=Figure 1. Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (A00804-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded wit Key=msrp_display_actual_price_type, value= Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=gene_full_name, value=oncostatin M Key=uniprot_id, value=Q65Z15 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Key=sensitivity Key=applications, value=ELISA, WB Key=reactivity, value=Mouse, Rat Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins. Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot. Key=predicted_reactivity Key=source_company, value=Boster A00804-1 Key=isotype, value=Rabbit IgG Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway Key=clonality, value=Polyclonal Key=swatch_image, value=/a/0/a00801-2-faah-primary-antibodies-wb-testing-1.jpg Key=rating_value, value=100 Key=review_count, value=5 Key=phospho_site Key=mp_exclude_sitemap, value=Yes Key=yoast_facebook_image, value=/a/0/a00801-2-faah-primary-antibodies-wb-testing-1.jpg Key=yoast_twitter_image, value=/a/0/a00801-2-faah-primary-antibodies-wb-testing-1.jpg Key=cj_related_products, value=Kaposi Sarcoma:EK0367|PB9732|EZ0478|A00804|EZ0479 Key=description, value=Boster Bio Anti-Oncostatin M/Osm Antibody Picoband® catalog # A00804-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-Oncostatin M/Osm Antibody Picoband® catalog # A00804-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-Oncostatin M/Osm Antibody Picoband® Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Key=background, value=OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. OSM has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene. Key=gene_name, value=Osm Key=synonyms, value=Oncostatin-M; OSM; Osm Key=application_details, value=Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Key=subcellular_localization, value= Secreted. Key=tissue_specificity, value= Widely expressed. Expressed at higher levels in liver, skin and spleen. Key=protein_function, value= Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (By similarity). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Key=immunogen, value=E. coli-derived rat Oncostatin M recombinant protein (Position: K26-R208). Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=28 kDa Key=custom_attribute_2, value=WB: rat lung tissue, rat spleen tissue, mouse lung tissue, mouse spleen tissue Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=quantity_and_stock_status Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-Oncostatin M/Osm Antibody Picoband®
Oncostatin M/OSM antibody
Boster Bio Anti-Oncostatin M/Osm Antibody Picoband® catalog # A00804-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).
Boster Bio Anti-Oncostatin M/Osm Antibody Picoband® catalog # A00804-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Oncostatin M/Osm Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00804-1)
E. coli-derived rat Oncostatin M recombinant protein (Position: K26-R208).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00804-1 is reactive to Osm in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
28.484kDa
Background of Oncostatin M/OSM
OSM (ONCOSTATIN M) is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. OSM is mapped on 22q12.2. OSM has the ability to inhibit the growth of human A375 melanoma cells but not normal human fibroblasts. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM also has the ability to inhibit the proliferation of murine M1 myeloid leukemic cells and can induce their differentiation into macrophage-like cells, a function shared by LIF, CSF3, and IL6. The direction of gene transcription was telomeric to centromeric, with the OSM gene located upstream of the LIF gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: rat lung tissue, rat spleen tissue, mouse lung tissue, mouse spleen tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Oncostatin M using anti-Oncostatin M antibody (A00804-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Oncostatin M antigen affinity purified polyclonal antibody (Catalog # A00804-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Oncostatin M at approximately 28KD. The expected band size for Oncostatin M is at 28KD.
Gene/Protein Information For Osm (Source: Uniprot.org, NCBI)
Gene Name
Osm
Full Name
Oncostatin-M
Weight
28.484kDa
Superfamily
LIF/OSM family
Alternative Names
Oncostatin-M; OSM; Osm OSM oncostatin M oncostatin-M
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on Osm, check out the Osm Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Osm: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhu M, Che Q, Liao Y, Wang H, Wang J, Chen Z, Wang F, Dai C, Wan X. Oncol Rep. 2015 Jul;34(1):129-38. Doi: 10.3892/Or.2015.3951. Epub 2015 May 5. Oncostatin M Activates Stat3 To Promote Endometrial Cancer Invasion And Angiogenesis.
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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