Key=store_id
Key=entity_id, value=9193
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Key=sku, value=EZ0478
Key=has_options, value=0
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Key=created_at, value=2016-11-23 01:10:53
Key=updated_at, value=2024-09-26 00:48:25
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Human OSM/Oncostatin M ELISA Kit EZ-Set™ (DIY Antibody Pairs)
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Key=template, value=ez-set
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Key=yoast_content_score, value=60
Key=price, value=500.0000
Key=weight, value=1.0000
Key=meta_title, value=Human OSM/Oncostatin M Antibody Pairs ELISA Kit
Key=meta_description, value=Human OSM/Oncostatin M ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human OSM in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA)..
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Key=url_key, value=human-osm-oncostatin-m-elisa-kit-ez-set-trade-diy-antibody-pairs-ez0478-boster
Key=url_path, value=human-osm-oncostatin-m-elisa-kit-ez-set-trade-diy-antibody-pairs
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Key=image_label, value=Human OSM/Oncostatin M EZ Set ELISA Kit standard curve
Key=small_image_label, value=Human OSM/Oncostatin M EZ Set ELISA Kit standard curve
Key=thumbnail_label, value=Human OSM/Oncostatin M EZ Set ELISA Kit standard curve
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=For 5 plates, 96 wells each
Key=protein_name, value=Oncostatin-M
Key=gene_full_name, value=Oncostatin-M
Key=uniprot_id, value=P13725
Key=assay_range, value=15.6 pg/ml - 1,000 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=anticoagulant, value=heparin or EDTA
Key=tmb_incubation_time, value=20-25min
Key=normal_range_citations, value=24600984
Key=source_company, value=Boster EZ0478
Key=molecular_weight, value=28484 MW
Key=related_product_skus, value=PROTP13725,PROTP13725-1,PROTP53347,PROTQ65Z15,PROTP13725-2,PROTP13725-3,PROTP13725-4
Key=product_category, value=EZ-Set ELISA Kits (Ab Pairs)
Key=research_category, value=Cytokines, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Interleukins, Nuclear Signaling, Nuclear Signaling Pathways, Signal Transduction, Signaling Pathway
Key=swatch_image, value=/e/k/ek0478.png
Key=rating_value, value=100
Key=review_count, value=13
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Key=capture_antibody, value=Rabbit anti- human OSM polyclonal antibody
Key=detection_antibody, value=Biotinylated goat anti- human OSM polyclonal antibody
Key=cj_related_products, value=Janiceps:A00274-2^Kaposi Sarcoma:EK0577|EK0478|EK0479|A00804
Key=description, value=Human OSM/Oncostatin M ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human OSM in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Key=short_description, value=Human OSM/Oncostatin M ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human OSM in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Key=meta_keyword, value=Human OSM/Oncostatin M EZ-Set ELISA Kit DIY Antibody Pairs
Key=background, value=OSM (Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.
Key=gene_name, value=OSM
Key=synonyms, value=Oncostatin-M;OSM;OSM;
Key=sample_type, value=cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Key=kit_components, value=

Catalog number	Description	Quantity	Storage of opened/reconstituted material
EZ0478-CA	Rabbit anti- human OSM polyclonal antibody (Capture Antibody)	500 µl, 0.4 mg/mL (recommended dilution 1:100)	Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0478-DA	Biotinylated goat anti- human OSM polyclonal antibody (Detection Antibody)	500 µl, 2.5 µg/mL (recommended dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	500 µl (recommended dilution 1:100)
EK0478-ST	Lyophilized recombinant human OSM standard	10 ng/tube×3	Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	104	351	627	135	392	658
Standard deviation	5.72	16.15	42.64	8.37	20.78	47.4
CV (%)	5.5%	4.6%	6.8%	6.2%	5.3%	7.2%

Key=componentsneeded, value=

1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate (Cat# AR1100)
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered.
10. Color Developing Reagent: Tetramethylbenzidine (Cat# AR1104)
11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
12. Wash Buffer (PBS and PBS-T).
  PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered.
  PBS-T: 0.1% Tween? 20 in PBS, pH 7.2-7.4.
*Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

Key=subcellular_localization, value= Secreted.
Key=protein_function, value= Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration (By similarity). .
Key=sample_data, value=

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.023	0.113	0.21	0.332	0.589	1.062	1.574	2.251

Key=description_before_attributes, value=

Product Features:

Your ideal low cost ELISA solution. EZ Set™ ELISA kit contains the necessary core reagents develop and run the ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC). One kit is sufficient for developing 5 plates (96 well format). EZ Set™ ELISAs are ideal for experienced ELISA users on a budget and great for exploratory work--a fast and easy way to identify the relevant protein targets of interest/relevance to your studies.

Boster also provides the most comprehensive technical support materials and most user friendly technical support service to guarantee that you can do the experiment smoothly and successfully.

Key=competitor_equivalent_skus, value=sc 27104|sc 50294|sc 5488|sc 374040|sc 373734|sc 133229|sc 374039|sc 129|sc 50296|sc 365136|sc 390253
Key=immunogen, value=Expression system for standard: E.coli; Immunogen sequence: A26R-220
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=preparation_step_2, value=1) It is recommended that the standards be prepared no more than 2 hours prior to performing the experiment. Use one 10 ng of lyophilized human OSM standard for each experiment. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10 ng/ml using 1 ml of Reagent Diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions.
2) Dilution of Human OSM Standard
• Number tubes 1 - 8. Final Concentrations to be Tube # 1 - 1,000 pg/ml, # 2 - 500 pg/ml, # 3 - 250 pg/ml, # 4 - 125 pg/ml, # 5 - 62.5 pg/ml, # 6 - 31.2 pg/ml, # 7 - 15.6 pg/ml, # 8 - 0.0 (Blank).
• To generate standard # 1, add 100 µl of the reconstituted standard stock solution of 10 ng/ml and 900 µl of Reagent Diluent to tube # 1 for a final volume of 1,000 µl. Mix thoroughly.
• Add 300 µl of Reagent Diluent to tubes # 2 - 7.
• To generate standard # 2, add 300 µl of standard # 1 from tube # 1 to tube # 2 for a final volume of 600 µl. Mix thoroughly.
• To generate standard # 3, add 300 µl of standard # 2 from tube # 2 to tube # 3 for a final volume of 600 µl. Mix thoroughly.
• Continue the serial dilution for tube # 4 - 7.
• Tube # 8 is a blank standard to be used with every experiment.
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