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Product Info Summary
| SKU: | EK0464 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin) and saliva. |
Product info
Product Name
Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine®
SKU/Catalog Number
EK0464
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MMP-8 ELISA Kit PicoKine® (96 Tests). Quantitate Human MMP8 in cell culture supernatants, serum, plasma (heparin) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0464)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F21-G467
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0464 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0464 is reactive to MMP8 in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin) and saliva.
Application Guarantee
EK0464 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-8
Matrix metalloproteinase 8 (MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase (HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0464-CAP | Anti-Human MMP8 Pre-coated 96-well strip microplate | 1 |
| EK0464-ST | Human MMP8 Standard | 2 vials, 10 ng/tube |
| EK0464-DA | Human MMP8 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.083 | 0.334 | 0.486 | 0.740 | 1.181 | 1.468 | 1.673 | 1.930 |
Data Example Images
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Human MMP-8 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-8, Dilution ratio of 1:30, concentration in serum and plasma is around 40 ng/ml..
Some articles we found to cite concentrations of MMP-8 in samples: 17546376 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 251 | 1516 | 5010 | 258 | 1538 | 5119 |
| Standard deviation | 13.05 | 101.57 | 360.72 | 18.31 | 109.19 | 404.4 |
| CV (%) | 5.2% | 6.7% | 7.2% | 7.1% | 7.1% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MMP-8 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 251 | 247 | 275 | 273 | 261 | 12.59 | 4.8% |
| Sample 2 | 1516 | 1312 | 1347 | 1465 | 1410 | 83.41 | 5.9% |
| Sample 3 | 5010 | 5134 | 4821 | 4702 | 4916 | 166.71 | 3.3% |
Protein Target Info & Infographic
Gene/Protein Information For MMP8 (Source: Uniprot.Org, NCBI)
Gene Name
MMP8
Full Name
Neutrophil collagenase
Weight
53412 MW
Superfamily
peptidase M10A family
Alternative Names
Neutrophil collagenase;3.4.24.34;Matrix metalloproteinase-8;MMP-8;PMNL collagenase;PMNL-CL;MMP8;CLG1; MMP8 CLG1, HNC, MMP-8, PMNL-CL matrix metallopeptidase 8 neutrophil collagenase|PMN leukocyte collagenase|PMNL collagenase|collagenase 2|matrix metalloproteinase 8 (neutrophil collagenase)|matrix metalloproteinase-8
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MMP8, check out the MMP8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MMP8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine® (EK0464)
Hello CJ!
EK0464 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Transplantation of human amniotic epithelial cells promotes morphological and functional regeneration in a rat uterine scar model
A rapid, non-invasive tool for periodontitis screening in a medical care setting
Comparison of regular- and slope-configured dental implants placed in the edentulous ridge with height discrepancy: A pilot randomized controlled trial
Clinical outcomes of adjunctive indocyanine green-diode lasers therapy for treating refractory periodontitis: A randomized controlled trial with in vitro assessment
Randomized controlled clinical effectiveness of adjunct 660-nm light-emitting diode irradiation during non-surgical periodontal therapy
Tu CC, Wang SH, Hu CY, Chen MH, Hsieh O, Chang PC. Comparison of regular- and slope-configured dental implants placed in the edentulous ridge with height discrepancy: A pilot randomized controlled trial. J Formos Med Assoc. 2020 Nov 5:S0929-6646(20)30503-
Species: Human
EK0464 usage in article: APP:ELISA, SAMPLE:GINGIVAL CERVICULAR FLUID, DILUTION:NA
Expression of matrix metalloproteinase-8 and matrix metalloproteinase-13 in mast cells of human periapical lesions
Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test
Kumar Y, Jain V, Chauhan SS, Bharate V, Koli D, Kumar M. J Prosthet Dent. 2017 Mar 23. pii: S0022-3913(16)30693-X. doi: 10.1016/j.prosdent.2016.11.017. Influence of different forms and materials (zirconia or titanium) of abutments in periimplant s...
Stio M, Martinesi M, Treves C, Borgioli F. J Mater Sci Mater Med. 2015 Feb;26(2):100. Doi: 10.1007/S10856-015-5446-Y. Epub 2015 Feb 6. In Vitro Response Of Human Peripheral Blood Mononuclear Cells To Aisi 316L Austenitic Stainless Steel Subjected ...
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Human MMP-8/Neutrophil collagenase ELISA Kit PicoKine®
Question
Q: can I use EDTA plasma as samples in Human MMP-8 Picokine® ELISA Kit (Catalog # EK0464)?
Verified Customer
Verified customer
Asked: 2020-05-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of MMP-8 causing disruption of its protein structure. MMP-8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the MMP-8 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-05-30
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
M. Dhar
Verified customer
Asked: 2019-10-07
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-10-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-25
Question
How many reactions are included in one EK0464 kit?
Verified customer
Asked: 2019-03-28
Answer
There are 12 strips of 8 wells each in the Human MMP-8 ELISA Kit PicoKine™ (EK0464), all removable from the plate. The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit.
Boster Scientific Support
Answered: 2019-03-28
Question
Are the antibodies in EK0464 suitable for Western Blot application?
Verified customer
Asked: 2019-03-28
Answer
The antibody in the Human MMP-8 ELISA Kit PicoKine™ (EK0464) and all our ELISA kits are not validated for Western Blot usage.
Boster Scientific Support
Answered: 2019-03-28
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-10-28
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-10-28
Question
Q: how to analyze ELISA data? I have obtained MMP-8 level in saliva.
H. Lopez
Verified customer
Asked: 2018-02-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-02-09
Question
Q: if the enzyme conjugated MMP-8 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-8 antigen?
Verified Customer
Verified customer
Asked: 2017-10-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2017-10-25
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
H. Singh
Verified customer
Asked: 2015-08-19
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2015-08-19
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of MMP-8 in Human saliva? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
N. Jones
Verified customer
Asked: 2014-01-27
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the MMP-8 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2014-01-27
