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Product Info Summary
| SKU: | RP1007 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Growth Hormone/GH1 Antibody Picoband®
View all Growth Hormone Antibodies
SKU/Catalog Number
RP1007
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Growth Hormone/GH1 Antibody catalog # RP1007. Tested in ELISA, IHC, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Growth Hormone/GH1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1007)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived rat GH recombinant protein (Position: F27-F216).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1007 is reactive to Gh1 in Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
22 kDa
Calculated molecular weight
24656 MW
Background of Growth Hormone
Growth Hormone (GH) is mapped to 17q22-q24. Human growth hormone has a molecular mass of 22,005 and contains 191 amino acid residues with 2 disulfide bridges. Rat GH shares 98% amino acid sequence homology with mouse. It binds two receptor molecules and thereby induces signal transduction through receptor dimerization. At high concentrations, GH acts as an antagonist because of a large difference in affinities at the respective binding sites.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1007 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat, By Heat
ELISA, 0.1-0.5μg/ml, -
Positive Control
IHC: rat brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GH1 using anti-GH1 antibody (RP1007).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 5 ng of sample under reducing conditions.
Lane 1: rat GH1 recombinant protein.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GH1 antigen affinity purified polyclonal antibody (Catalog # RP1007) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GH1 at approximately 22 kDa.
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Figure 2. IHC analysis of GH1 using antiGH1 antibody (RP1007).
GH1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GH1 Antibody (RP1007) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Gh1 (Source: Uniprot.org, NCBI)
Gene Name
Gh1
Full Name
Somatotropin
Weight
24656 MW
Superfamily
somatotropin/prolactin family
Alternative Names
Somatotropin;Growth hormone;Gh1;Gh; GH1 GH, GH-N, GHB5, GHN, IGHD1A, IGHD1B, IGHD2, hGH-N growth hormone 1 somatotropin|growth hormone B5|pituitary growth hormone
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Gh1, check out the Gh1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Gh1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Growth Hormone/GH1 Antibody Picoband® (RP1007)
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