Click image to see more details
Product Info Summary
| SKU: | A06059 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-POLR3A Antibody
SKU/Catalog Number
A06059
Size
100μl
Form
Liquid
Description
Boster Bio Anti-POLR3A Antibody catalog # A06059. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLR3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06059)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RPC1. AA range:31-80
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06059 is reactive to POLR3A in Human, Monkey, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
155641 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06059 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of specific cells using POLR3A Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For POLR3A (Source: Uniprot.org, NCBI)
Gene Name
POLR3A
Full Name
DNA-directed RNA polymerase III subunit RPC1
Weight
155641 MW
Superfamily
RNA polymerase beta' chain family
Alternative Names
DNA-directed RNA polymerase III subunit RPC1;RNA polymerase III subunit C1;2.7.7.6;DNA-directed RNA polymerase III largest subunit;DNA-directed RNA polymerase III subunit A;RNA polymerase III 155 kDa subunit;RPC155;RNA polymerase III subunit C160;POLR3A; POLR3A ADDH, C160, HLD7, RPC1, RPC155, WDRTS, hRPC155 RNA polymerase III subunit A DNA-directed RNA polymerase III subunit RPC1|DNA-directed RNA polymerase III largest subunit|DNA-directed RNA polymerase III subunit A|RNA polymerase III 155 kDa subunit|RNA polymerase III subunit C1|RNA polymerase III subunit C160|RNA polymerase III subunit RPC155-D|polymerase (RNA) III (DNA directed) polypeptide A, 155kDa|polymerase (RNA) III subunit A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR3A, check out the POLR3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR3A Antibody (A06059)
Hello CJ!
No publications found for A06059
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-POLR3A Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-POLR3A Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-POLR3A Antibody
Question
I see that the anti-POLR3A antibody A06059 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-12-09
Question
Our lab want to know about to test anti-POLR3A antibody A06059 on human buccal mucosa cell for research purposes, then I may be interested in using anti-POLR3A antibody A06059 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The products we sell, including anti-POLR3A antibody A06059, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-30
Question
Would A06059 anti-POLR3A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
As indicated on the product datasheet, A06059 anti-POLR3A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-02
Question
Here is the WB image, lot number and protocol we used for buccal mucosa cell using anti-POLR3A antibody A06059. Please let me know if you require anything else.
K. Lewis
Verified customer
Asked: 2018-09-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for buccal mucosa cell using anti-POLR3A antibody A06059. Let me know if you need anything else.
S. Banerjee
Verified customer
Asked: 2017-07-13
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-07-13
Question
I was wanting to use your anti-POLR3A antibody for WB for human buccal mucosa cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human buccal mucosa cell identification?
D. Baker
Verified customer
Asked: 2015-06-30
Answer
You can see on the product datasheet, A06059 anti-POLR3A antibody has been validated for WB on human, monkey, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human buccal mucosa cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-30
Question
Is this A06059 anti-POLR3A antibody reactive to the isotypes of POLR3A?
R. Carter
Verified customer
Asked: 2014-02-28
Answer
The immunogen of A06059 anti-POLR3A antibody is Synthesized peptide derived from the N-terminal region of human POLR3A. at AA rangle: 1-80. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-28
