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Product Info Summary
| SKU: | EK1259 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
Product info
Product Name
Human Ficolin-3 ELISA Kit PicoKine®
SKU/Catalog Number
EK1259
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Ficolin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human FCN3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Ficolin-3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1259)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q24-R299
Sensitivity
<20 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK1259 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1259 is reactive to FCN3 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK1259 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCN3
Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1259-CAP | Anti-Human FCN3 Pre-coated 96-well strip microplate | 1 |
| EK1259-ST | Human FCN3 Standard | 2 vials, 50 ng/tube |
| EK1259-DA | Human FCN3 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.037 | 0.112 | 0.216 | 0.370 | 0.603 | 1.076 | 1.362 | 1.842 |
Data Example Images
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Human Ficolin-3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FCN3, For serum, dilution ratio is 1:1 and concentration is around 7 ng/ml.
For plasma, dilution ratio is 1:5 and concentration is around 15 ng/ml..
Some articles we found to cite concentrations of FCN3 in samples: 22206485, 19208603 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Ficolin-3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1216 | 5741 | 26480 | 1219 | 6253 | 25739 |
| Standard deviation | 80.25 | 396.12 | 1403.44 | 80.45 | 450.21 | 1853.2 |
| CV (%) | 6.6% | 6.9% | 5.3% | 6.6% | 7.2% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of FCN3 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 1216 | 1347 | 1267 | 1181 | 1252 | 62.41 | 4.9% |
| Sample 2 | 5741 | 5533 | 5876 | 6484 | 5908 | 354.01 | 5.9% |
| Sample 3 | 26480 | 26566 | 26529 | 22150 | 25431 | 1894.67 | 7.4% |
Protein Target Info & Infographic
Gene/Protein Information For FCN3 (Source: Uniprot.Org, NCBI)
Gene Name
FCN3
Full Name
Ficolin-3
Weight
32903 MW
Superfamily
ficolin lectin family
Alternative Names
Ficolin-3;Collagen/fibrinogen domain-containing lectin 3 p35;Collagen/fibrinogen domain-containing protein 3;Hakata antigen;FCN3;FCNH, HAKA1; FCN3 FCNH, HAKA1 ficolin 3 ficolin-3|H-ficolin|collagen/fibrinogen domain-containing lectin 3 p35|collagen/fibrinogen domain-containing protein 3|ficolin (collagen/fibrinogen domain containing) 3 (Hakata )
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on FCN3, check out the FCN3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCN3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Ficolin-3 ELISA Kit PicoKine® (EK1259)
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Customer Q&As
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9 Customer Q&As for Human Ficolin-3 ELISA Kit PicoKine®
Question
What is the immunogen sequence of EK1259?
Verified customer
Asked: 2021-01-12
Answer
The immunogen sequence of the Human FCN3 Standard in EK1259 is Q24-R299.
Boster Scientific Support
Answered: 2021-01-12
Question
Is the standard of EK1259 full-length Ficolin-3, fragment or a peptide?
Verified customer
Asked: 2021-01-12
Answer
For the Human Ficolin-3 ELISA Kit PicoKine™ (EK1259), the standard is full length Ficolin-3.
Boster Scientific Support
Answered: 2021-01-12
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-09-07
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-09-07
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-23
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-23
Question
Q: if the enzyme conjugated FCN3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FCN3 antigen?
Y. Lewis
Verified customer
Asked: 2020-02-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-02-22
Question
What is the recommended dilution ratio for human plasma samples with EK1259?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
The suggested dilution ratio is 1:100-1:1000 for human plasma samples.
Boster Scientific Support
Answered: 2020-01-03
Question
Q: can you recommend the dilution ratio of serum samples for detection of FCN3 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-11-18
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the FCN3 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-18
Question
Q: can I use citrate plasma as samples in Human FCN3 Picokine® ELISA Kit (Catalog # EK1259)?
V. Robinson
Verified customer
Asked: 2018-03-09
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of FCN3 causing disruption of its protein structure. FCN3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the FCN3 ELISA, treating samples with a number of anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-03-09
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
R. Zhao
Verified customer
Asked: 2015-01-12
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-01-12
