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Product Info Summary
| SKU: | EK0729 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CXCL7/PPBP/NAP-2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0729
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CXCL7/NAP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PPBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CXCL7/PPBP/NAP-2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0729)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A59-D128
Sensitivity
<2 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0729 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0729 is reactive to PPBP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0729 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of PPBP
Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0729-CAP | Anti-Human PPBP Pre-coated 96-well strip microplate | 1 |
| EK0729-ST | Human PPBP Standard | 2 vials, 10 ng/tube |
| EK0729-DA | Human PPBP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.026 | 0.058 | 0.114 | 0.189 | 0.320 | 0.605 | 1.211 | 1.812 |
Data Example Images
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Human CXCL7 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect PPBP, Dilution ratio of 1:500, concentration in serum and plasma is 50-150 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CXCL7/PPBP/NAP-2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 29 | 155 | 411 | 28 | 157 | 395 |
| Standard deviation | 1.47 | 7.9 | 29.18 | 1.73 | 9.26 | 32.78 |
| CV (%) | 8.3% | 8.3% | 7.1% | 6.2% | 5.9% | 8.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of PPBP in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 31 | 30 | 30 | 30 | 0.7 | 2.3% |
| Sample 2 | 155 | 156 | 165 | 162 | 159 | 4.15 | 2.6% |
| Sample 3 | 411 | 409 | 455 | 402 | 419 | 20.9 | 4.9% |
Protein Target Info & Infographic
Gene/Protein Information For PPBP (Source: Uniprot.Org, NCBI)
Gene Name
PPBP
Full Name
Platelet basic protein
Weight
13894 MW
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
Platelet basic protein;PBP;C-X-C motif chemokine 7;Leukocyte-derived growth factor;LDGF;Macrophage-derived growth factor;MDGF;Small-inducible cytokine B7;Connective tissue-activating peptide III;CTAP-III;LA-PF4;Low-affinity platelet factor IV;TC-2;Connective tissue-activating peptide III (1-81);CTAP-III (1-81);Beta-thromboglobulin;Beta-TG;Neutrophil-activating peptide 2 (74);NAP-2 (74);Neutrophil-activating peptide 2 (73);NAP-2 (73);Neutrophil-activating peptide 2;NAP-2;TC-1;Neutrophil-activating peptide 2 (1-66);NAP-2 (1-66);Neutrophil-activating peptide 2 (1-63);NAP-2 (1-63);PPBP;CTAP3, CXCL7, SCYB7, TGB1, THBGB1; PPBP B-TG1, Beta-TG, CTAP-III, CTAP3, CTAPIII, CXCL7, LA-PF4, LDGF, MDGF, NAP-2, PBP, SCYB7, TC1, TC2, TGB, TGB1, THBGB, THBGB1 pro-platelet basic protein platelet basic protein|C-X-C motif chemokine 7|CXC chemokine ligand 7|beta-thromboglobulin|chemokine (C-X-C motif) ligand 7|connective tissue-activating peptide III|leukocyte-derived growth factor|low-affinity platelet factor IV|macrophage-derived growth factor|neutrophil-activating peptide 2|small inducible cytokine B7|small inducible cytokine subfamily B, member 7|thrombocidin 1|thrombocidin 2|thromboglobulin, beta-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PPBP, check out the PPBP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PPBP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CXCL7/PPBP/NAP-2 ELISA Kit PicoKine® (EK0729)
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Human CXCL7/PPBP/NAP-2 ELISA Kit PicoKine®
Question
Q: how to proceed with the analysis of ELISA data? I have obtained PPBP level in plasma.
Z. Zhao
Verified customer
Asked: 2020-10-08
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-10-08
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
P. Maharaj
Verified customer
Asked: 2020-06-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-06-08
Question
Q: can you recommend the dilution ratio of serum samples for detection of PPBP in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-05-15
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the PPBP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-05-15
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-01-09
Question
Q: can I use citrate plasma as samples in Human PPBP Picokine® ELISA Kit (Catalog # EK0729)?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of PPBP causing disruption of its protein structure. PPBP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the PPBP ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-24
Question
Q: how can I thaw whole blood sample for PPBP ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test PPBP for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-08-26
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
K. Davis
Verified customer
Asked: 2018-11-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-11-06
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
C. Walker
Verified customer
Asked: 2014-04-27
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-04-27
