Human Ficolin-3 PicoKine® Quick ELISA Kit

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Ficolin-3 PicoKine® Quick ELISA Kit

See all FCN3 products

SKU/Catalog Number

FEK1259

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Ficolin-3 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Human FCN3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Ficolin-3 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1259)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: NS0; Immunogen sequence: Q24-R299

Sensitivity

<20 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Standard Dilution Instructions

See datasheet of FEK1259 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1259 is reactive to FCN3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

FEK1259 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of FCN3

Ficolin-3, also known as HAKA1, is a protein that in humans is encoded by the FCN3 gene. It is mapped to 1p36.11. The protein can activate the complement pathway in association with MASPs and sMAP, thereby aiding in host defense through the activation of the lectin pathway. Ficolin-3 is expressed as a 35-kD protein that reacts with systemic lupus erythematosus sera. Ficolin-3 does not bind fibronectin (FN1), elastin (ELN), or zymosan, and the lectin activity of the Ficolin-3 is calcium independent. Homozygosity for the variant in the FCN3 gene results in a recessive complement deficiency syndrome.

Kit Components

Catalog number	Description	Quantity
FEK1259-CAP	96-well plate precoated with anti-Human FCN3 antibody	1
EK1259-ST	lyophilized recombinant Human FCN3 standard	50 ng/tube
FEK1259-DA	Biotinylated anti-Human FCN3 detection antibody (50x)	100 ul
-	Avidin-Biotin-Peroxidase Complex (30x)	400 µl
AR1106-1	Sample diluent	30 ml
AR1106-2	Antibody diluent	12 ml
-	Avidin-Biotin-Peroxidase Diluent	12 ml
AR1106-5	Wash Buffer (25x)	20 ml
AR1104	Color Developing Reagent (TMB)	10 ml
AR1105	Stop Solution	10 ml
PLA-SEA	Plate Sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.159	0.188	0.239	0.323	0.494	0.817	1.264	1.915

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Ficolin-3 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FCN3 in ELISA kits from four different production batches/lots.

Gene/Protein Information For FCN3 (Source: Uniprot.Org, NCBI)

Alternative Names

Ficolin-3;Collagen/fibrinogen domain-containing lectin 3 p35;Collagen/fibrinogen domain-containing protein 3;Hakata antigen;FCN3;FCNH, HAKA1; FCN3 FCNH, HAKA1 ficolin 3 ficolin-3|H-ficolin|collagen/fibrinogen domain-containing lectin 3 p35|collagen/fibrinogen domain-containing protein 3|ficolin (collagen/fibrinogen domain containing) 3 (Hakata )

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

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