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Product Info Summary
| SKU: | EK0867 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0867
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine® (96 Tests). Quantitate Human DKK1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0867)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: T32-H266
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0867 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0867 is reactive to DKK1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0867 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Dkk-1
Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0867-CAP | Anti-Human DKK1 Pre-coated 96-well strip microplate | 1 |
| EK0867-ST | Human DKK1 Standard | 2 vials, 10 ng/tube |
| EK0867-DA | Human DKK1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.012 | 0.107 | 0.187 | 0.402 | 0.747 | 1.268 | 1.599 | 2.006 |
Data Example Images
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Human DKK-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Dkk-1, For serum, dilution ratio is 1:20 and concentration is around 9 ng/ml.
For plasma, dilution ratio is 1:1 and concentration is 99-978 pg/ml..
Some articles we found to cite concentrations of Dkk-1 in samples: 25941031, 25877354, 24612589 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 108 | 660 | 1140 | 103 | 594 | 1134 |
| Standard deviation | 5.72 | 47.52 | 66.12 | 6.59 | 43.36 | 82.78 |
| CV (%) | 5.3% | 7.2% | 5.8% | 6.4% | 7.3% | 7.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Dkk-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 108 | 125 | 119 | 115 | 116 | 6.17 | 5.3% |
| Sample 2 | 660 | 564 | 616 | 629 | 617 | 34.65 | 5.6% |
| Sample 3 | 1140 | 1087 | 1155 | 1110 | 1123 | 26.35 | 2.3% |
Protein Target Info & Infographic
Gene/Protein Information For DKK1 (Source: Uniprot.Org, NCBI)
Gene Name
DKK1
Full Name
Dickkopf-related protein 1
Weight
28672 MW
Superfamily
dickkopf family
Alternative Names
Dickkopf-like protein 1 ;DKK1 ; Dkk1|mdkk-1|dickkopf WNT signaling pathway inhibitor 1|dickkopf-related protein 1|dickkopf homolog 1|dickkopf-1|dickkopf-like protein 1|dkk-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on DKK1, check out the DKK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DKK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine® (EK0867)
Hello CJ!
EK0867 has been cited in 10 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
DKK1 in relation to HCV induced liver cirrhosis and HCV induced HCC curative resection.
Dickkopf-1 Is a Biomarker for Systemic Lupus Erythematosus and Active Lupus Nephritis
Serum midkine is a more sensitive predictor for hepatocellular carcinoma than Dickkopf-1 and alpha-L-fucosidase in cirrhotic HCV patients
Combination of serum tumor markers dickkopf-1, DCP and AFP for the diagnosis of primary hepatocellular carcinoma
Wnt signaling pathway in early- and late-onset preeclampsia: evaluation with Dickkopf-1 and R-Spondin-3 glycoproteins
El-Shayeb AF,El-Habachi NM,Mansour AR,Zaghloul MS.Serum midkine is a more sensitive predictor for hepatocellular carcinoma than Dickkopf-1 and alpha-L-fucosidase in cirrhotic HCV patients.Medicine (Baltimore).2021 Apr 30;100(17):e25112.doi:10.1097/MD.0000000000025112.PMID:33907088;PMCID:PMC8084028.
Species: Human
EK0867 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Qi L,Chen J,Zhou B,Xu K,Wang K,Fang Z,Shao Y,Yuan Y,Zheng S,Hu W.HomeoboxC6 promotes metastasis by orchestrating the DKK1/Wnt/β-catenin axis in right-sided colon cancer.Cell Death Dis.2021 Apr 1;12(4):337.doi:10.1038/s41419-021-03630-x.PMID:33795652.
Species: Human,Mouse
EK0867 usage in article: APP:ELISA, SAMPLE:HCT116 CELL, DILUTIO N:NA
Huo Q,Xu C,Shao Y,Yu Q,Huang L,Liu Y,Bao H.Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway.Int J Biol Sci 2021; 17(2):574-588.doi:10.7150/ijbs.52097
Species: Human,Mouse
EK0867 usage in article: APP:ELISA, SAMPLE:OVARIAN CANCER CELL, DILUTION:NA
DKK1 in relation to HCV induced liver cirrhosis and HCV induced HCC curative resection.
Combination of serum tumor markers dickkopf-1, DCP and AFP for the diagnosis of primary hepatocellular carcinoma
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Customer Reviews
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Customer Q&As
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Submit your question
5 Customer Q&As for Human DKK-1 / Dickkopf 1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-04-13
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Dkk-1 in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Dkk-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-09-03
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2018-12-09
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-12-09
Question
Q: how to proceed with the analysis of ELISA data? I measured Dkk-1 level in cell lysates.
T. Krishna
Verified customer
Asked: 2018-05-10
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-10
Question
Q: which procedure should I follow in order to thaw whole blood sample for Dkk-1 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2017-10-18
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Dkk-1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2017-10-18
