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- Table of Contents
10 Citations 5 Q&As
1 Citations 11 Q&As
Facts about Dickkopf-related protein 1.
Plays a role in limb development; attenuates Wnt signaling in the developing limb to permit normal limb patterning (PubMed:18505822). .
Mouse | |
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Gene Name: | Dkk1 |
Uniprot: | O54908 |
Entrez: | 13380 |
Belongs to: |
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dickkopf family |
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like
Mass (kDA):
29.298 kDA
Mouse | |
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Location: | 19|19 C1 |
Sequence: | 19; |
We can help you determine DKK1 protein in your samples, or provide you with information regarding DKK1 marker. Boster Bio offers validated ELISA kits as well as primary and second antibodies to this particular target. Find out more about their products and how they can aid you. We will also look at the benefits of ELISA including its picogram sensitiveness.
The membrane's principal component, the DKK1 protein, is bound to the DKK1 kinase. Antibodies to DKK1 can be found as primary and secondary antibodies. The primary antibody was developed by Abcam and the secondary antibody was produced by Santa Cruz Biotechnology. The antibody was incubated for 15 mins. The primary antibody was used as a loading control and the secondary antibody was added with the antibody to detect the protein.
This primary antibody was not approved for clinical diagnostic purposes. It is employed in research to screen for drugs or to determine if the treatment being used is producing undesirable side effects. The antibody comes with a 1-year guarantee. These antibodies can be used in research studies that are focused on DKK1 expression. To determine the knockdown efficiency of DKK1 they can be purchased from Santa Cruz Biotechnology.
Dickkopf-1 is a key regulator for human esophageal carcinoma. It also regulates the expression of ALDH2A1, which is a stem-like genetic gene, and can be used to predict outcomes for patients. It prevents the proliferation and vimentin formation of CK18 cells, CK18, SOX2, Bmi1 and CK18 cells. It also aids in the detoxification process of chemotherapy agents.
Additionally, DKK1 also controls the self-renewing capabilities of EC cells. In a subcutaneous-xenograft model, DKK1 knockdown was able to increase the survival rate of mice. In addition, DKK1-dependent activation ALDH2A1 plays a crucial role in the increase of tumor-initiating capabilities in EC cells. The primary antibody against DKK1 is extremely specific.
Boster Bio's picogram sensitivity has been enhanced with the use of proprietary coatings and blocking technologies. These kits are validated against cell culture supernatants as well as biologically relevant matrices. Furthermore, all Picokine ELISA kits are rigorously tested for intra-assay and inter-assay variability. These ELISA kits include technical support as well as free ELISA samples.
Boster Bio was founded by Steven Xia, a renowned histologist. They specialize in the production of high-specificity antibodies and sensitive antibodies. Boster Bio has over 12,000 antibodies in its range of products and has earned the trust of scientists from all over the world. Boster Bio's tested, high-quality antibodies have been referenced more than 14,000 times. And all of its products are covered by the a Boster Quality Guarantee.
These kits come in various formats, including microliter-scale versions. Boster Bio's multiplex ELISA kit can detect up to 18 analytes. It requires 25 microliters of sample and is 30% less expensive than traditional multiplex assays. Boster Bio's IQELISA (tm) product is a high-sensitivity picogram ELISA kits that utilize ultrasensitive DNA amplification in order to increase the sensitivity of detection.
For many years, Boster Bio has specialized in sandwich ELISA and multiplex ELISA kits to aid in research, diagnostics and clinical trials. The kits are easily available and have been validated by Boster Bio for their superior quality and reliability. These kits are also compatible with other imaging devices that are not Quansys. With high-sensitivity picogram ELISAs, Boster Bio's technology has revolutionized ELISA industry.
Human DKK1 is found in a variety of organs, tissues and tissues. This ELISA kit measures levels in human serum and cit plasma. This kit has a limit of 6.66 pg/ml of sensitivity, and is suitable for multiple reasons, such as research, diagnostics, and development. This kit does not cross-react with other relevant proteins.
The DKK1 ELISA Kit contains the following components: EK0867 CAP (human DKK1 antibody), EK0867 -ST (Human DKK1 Standard) and EK0867 -DA (biotinylated anti-bodies). The diluents, wash buffers and sanitizers are each 30ml. The adhesive sealers for plates are 20ml.
The immune system produces the enzyme. To produce activated p17 or P12 fragments, it needs proteolytic conversion. A aspartic acid residue located in the P1 position of the inactive zymogen is essential for the cleavage process of caspase. The antibodies test for myeloperoxidase (MPO) activity in patients with a suspicion of vasculitis caused by immune stimulation, or MPA.
TG-ROC analyses have shown that home-made and commercially available DKK1 ELISA kits have good agreement between their results. There are however some distinctions. Although the home-made kit was more sensitive but it was only moderately accurate in detecting infections within the Iranian population. The sensitivity of the homemade kit was superior to the commercial kit.
Boster Bio's DKK1 ELISA Kits were created by using zearalenone monoclonal antigen and an indirect ELISA. The detection range was between 1200ng/ml and R2 = 0.99. The 50% inhibition value of ZEN was 16.3 ng/ml. The average recovery rate for spiked corn and wheat samples was 96.7%. The coefficient of variation was 10.9 percent. The kits are suitable for storage at 4 degrees C for six months.
DKK1 ELISA kit can also be used to detect routinely in cases of ricin poisoning. This diagnostic kit can detect ricin in environmental and clinical samples. Additionally, it can also be used for assessing the effectiveness of therapeutic drugs. It is a crucial tool in the monitoring of drugs. However, there are many tests for this enzyme. Since DKK1 isn't a standard for reference most tests related to drugs be based on these kits as an excellent reference.
PMID: 9450748 by Glinka A., et al. Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction.
PMID: 17143291 by Niehrs C.; Function and biological roles of the Dickkopf family of Wnt modulators.
*More publications can be found for each product on its corresponding product page