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Product Info Summary
| SKU: | A00632 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-DKK1 Antibody Picoband®
SKU/Catalog Number
A00632
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DKK1 Antibody Picoband® catalog # A00632. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DKK1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00632)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human DKK1 recombinant protein (Position: T32-H266).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00632 is reactive to DKK1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
38 kDa
Calculated molecular weight
29.298kDa
Background of Dkk-1
DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00632 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DKK1 using anti-DKK1 antibody (A00632).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: recombinant human DKK1 protein 1ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 antigen affinity purified polyclonal antibody (Catalog # A00632) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DKK1 at approximately 38KD. The expected band size for DKK1 is at 26KD.
Protein Target Info & Infographic
Gene/Protein Information For DKK1 (Source: Uniprot.org, NCBI)
Gene Name
DKK1
Full Name
Dickkopf-related protein 1
Weight
29.298kDa
Superfamily
dickkopf family
Alternative Names
Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK; DKK1; UNQ492/PRO1008 Dkk1|mdkk-1|dickkopf WNT signaling pathway inhibitor 1|dickkopf-related protein 1|dickkopf homolog 1|dickkopf-1|dickkopf-like protein 1|dkk-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DKK1, check out the DKK1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DKK1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DKK1 Antibody Picoband® (A00632)
Hello CJ!
A00632 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Clusterin contributes to early stage of Alzheimer's disease pathogenesis
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-DKK1 Antibody Picoband®
Question
Would A00632 anti-DKK1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
You can see on the product datasheet, A00632 anti-DKK1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-23
Question
We are currently using anti-DKK1 antibody A00632 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
The anti-DKK1 antibody (A00632) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-13
Question
Is a blocking peptide available for product anti-DKK1 antibody (A00632)?
Verified Customer
Verified customer
Asked: 2019-10-30
Answer
We do provide the blocking peptide for product anti-DKK1 antibody (A00632). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-30
Question
Would anti-DKK1 antibody A00632 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
According to the expression profile of brain, DKK1 is highly expressed in brain. So, it is likely that anti-DKK1 antibody A00632 will work for WB with brain.
Boster Scientific Support
Answered: 2019-10-23
Question
I have a question about product A00632, anti-DKK1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00632 anti-DKK1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-11
Question
I was wanting to use your anti-DKK1 antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
You can see on the product datasheet, A00632 anti-DKK1 antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-26
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-DKK1 antibody A00632. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
My boss were happy with the WB result of your anti-DKK1 antibody. However we have seen positive staining in decidua secreted. using this antibody. Is that expected? Could you tell me where is DKK1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
From literature, decidua does express DKK1. Generally DKK1 expresses in secreted. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463
Boster Scientific Support
Answered: 2019-05-17
Question
Our lab used your anti-DKK1 antibody for WB on cerebellum in a previous experiment. I am using mouse, and We intend to use the antibody for ELISA next. We want examining cerebellum as well as leiomyosarcoma in our next experiment. Do you have any suggestion on which antibody would work the best for ELISA?
B. Wu
Verified customer
Asked: 2018-12-13
Answer
I took a look at the website and datasheets of our anti-DKK1 antibody and it appears that A00632 has been validated on mouse in both WB and ELISA. Thus A00632 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-12-13
Question
See below the WB image, lot number and protocol we used for brain using anti-DKK1 antibody A00632. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-12-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-11
Question
I see that the anti-DKK1 antibody A00632 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-12
Question
We have observed staining in human cerebellum. Do you have any suggestions? Is anti-DKK1 antibody supposed to stain cerebellum positively?
Verified Customer
Verified customer
Asked: 2017-07-18
Answer
From what I have seen in literature cerebellum does express DKK1. From what I have seen in Uniprot.org, DKK1 is expressed in decidua, leiomyosarcoma, fetal kidney, cerebellum, brain, among other tissues. Regarding which tissues have DKK1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Fetal kidney, Pubmed ID: 10570958
Leiomyosarcoma, Pubmed ID: 10383463
Boster Scientific Support
Answered: 2017-07-18
Question
My lab would like to test anti-DKK1 antibody A00632 on rat brain for research purposes, then I may be interested in using anti-DKK1 antibody A00632 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Yang
Verified customer
Asked: 2015-04-15
Answer
The products we sell, including anti-DKK1 antibody A00632, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-04-15
Question
Is this A00632 anti-DKK1 antibody reactive to the isotypes of DKK1?
L. Zhao
Verified customer
Asked: 2014-03-24
Answer
The immunogen of A00632 anti-DKK1 antibody is E. coli-derived human DKK1 recombinant protein (Position: T32-H266). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-03-24
Question
Is there a BSA free version of anti-DKK1 antibody A00632 available?
A. Krishna
Verified customer
Asked: 2013-09-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-DKK1 antibody A00632 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-09-03
