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Product Info Summary
| SKU: | EK1329 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Chemerin/RARRES2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1329
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Chemerin/RARRES2 ELISA Kit PicoKine® (96 Tests). Quantitate Human RARRES2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Chemerin/RARRES2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1329)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V17-S163
Sensitivity
<20 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1329 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1329 is reactive to RARRES2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1329 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Chemerin
Chemerin, also known as RARRES2 or TIG2, is a protein that in humans is encoded by the RARRES2 gene. It is mapped to 7q36.1. Chemerin is a potent chemoattractant specific for antigen-presenting cells that requires proteolytic activation and acts as a ligand for the G protein-coupled receptor CMKLR1 (also known as ChemR23). It is a 14 kDa protein secreted in an inactive form as prochemerin and is activated through cleavage of the C-terminus by inflammatory and coagulation serine proteases. Chemerin was found to stimulate chemotaxis of dendritic cells and macrophages to the site of inflammation. What’s more, the active protein has several roles, including that as an adipokine, and is truncated on both termini from the proprotein.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1329-CAP | Anti-Human RARRES2 Pre-coated 96-well strip microplate | 1 |
| EK1329-ST | Human RARRES2 Standard | 2 vials, 50 ng/tube |
| EK1329-DA | Human RARRES2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.000 | 0.375 | 0.700 | 0.852 | 1.200 | 1.472 | 1.858 | 2.042 |
Data Example Images
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Human Chemerin/RARRES2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Chemerin, Dilution ratio of 1:100, concentration in serum and plasma is around 100 ng/ml..
Some articles we found to cite concentrations of Chemerin in samples: 25664056, 25651894, 25539827 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Chemerin/RARRES2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 107 | 508 | 2037 | 113 | 519 | 1850 |
| Standard deviation | 5.02 | 32.53 | 154.86 | 6.81 | 42.05 | 172.13 |
| CV (%) | 4.7% | 6.4% | 7.6% | 6% | 8.1% | 9.3% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Chemerin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 100 | 121 | 111 | 116 | 112 | 7.77 | 6.9% |
| Sample 2 | 741 | 756 | 857 | 851 | 801 | 53.05 | 6.6% |
| Sample 3 | 2346 | 2384 | 2464 | 2369 | 2390 | 44.40 | 1.8% |
Protein Target Info & Infographic
Gene/Protein Information For RARRES2 (Source: Uniprot.Org, NCBI)
Gene Name
RARRES2
Full Name
Retinoic acid receptor responder protein 2
Weight
18618 MW
Alternative Names
Retinoic acid receptor responder protein 2;Chemerin;RAR-responsive protein TIG2;Tazarotene-induced gene 2 protein;RARRES2;TIG2; RARRES2 HP10433, TIG2 retinoic acid receptor responder 2 retinoic acid receptor responder protein 2|RAR-responsive protein TIG2|chemerin|retinoic acid receptor responder (tazarotene induced) 2|tazarotene-induced gene 2 protein
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on RARRES2, check out the RARRES2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for RARRES2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Chemerin/RARRES2 ELISA Kit PicoKine® (EK1329)
Hello CJ!
EK1329 has been cited in 6 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serum levels of chemerin, apelin, vaspin, and omentin-1 in obese type 2 diabetic Egyptian patients with coronary artery stenosis
The significance of blood and salivary oxidative stress markers and chemerin in gestational diabetes mellitus
Impact of chemerin, lipid profile, and insulin resistance on disease parameters in patients with multiple sclerosis
Circulating irisin and chemerin levels as predictors of seizure control in children with idiopathic epilepsy
Evaluation of Serum Resistin, Visfatin, and Chemerin Levels in Patients with Lung Cancer and Chronic Obstructive Pulmonary Disease, Mustafa Göktepe, Celalettin Korkmaz, Adil Zamani, Soner Demirbaş, and İbrahim KılınçTurkish Thoracic Journal.
Motawi, T.M.K., Mahdy, S.G., El-Sawalhi, M.M., Ali, E.N., & El-Telbany, R.F.A. (2017). Serum levels of chemerin, apelin, vaspin, and omentin-1 in obese type 2 diabetic Egyptian patients with coronary artery stenosis. Canadian Journal of Physiology...
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Human Chemerin/RARRES2 ELISA Kit PicoKine®
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-07-02
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-07-02
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-05
Question
Q: is it okay to use citrate plasma as samples in Human Chemerin Picokine® ELISA Kit (Catalog # EK1329)?
G. Thomas
Verified customer
Asked: 2020-02-01
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Chemerin causing disruption of its protein structure. Chemerin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Chemerin ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-02-01
Question
Q: if the enzyme conjugated Chemerin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Chemerin antigen?
M. Anand
Verified customer
Asked: 2019-04-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-29
Question
Is EK1329 polyclonal or monoclonal antibody?
Verified customer
Asked: 2019-04-03
Answer
The capture antibody for the Human Chemerin/RARRES2 ELISA Kit PicoKine™ (EK1329) is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat.
Boster Scientific Support
Answered: 2019-04-03
Question
Which chemerin isoforms does EK1329 recognizes? With which affinity?
Verified customer
Asked: 2019-04-03
Answer
The Human Chemerin/RARRES2 ELISA Kit PicoKine™ (EK1329) recognize isoforms containing the sequence of V17-S163.
Boster Scientific Support
Answered: 2019-04-03
Question
Which chemerin isoform has EK1329 been validated?
Verified customer
Asked: 2019-04-03
Answer
Our Human Chemerin/RARRES2 ELISA Kit PicoKine™ (EK1329) hasn't been validated with any chemerin isoform.
Boster Scientific Support
Answered: 2019-04-03
Question
Q: can you suggest the dilution ratio of serum samples for detection of Chemerin in Human plasma? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
L. Brown
Verified customer
Asked: 2019-02-26
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Chemerin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-02-26
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Chemerin using the EK1329 Human Chemerin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-06-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2018-06-26
Question
Q: Can Chemerin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
M. Lewis
Verified customer
Asked: 2018-03-05
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-03-05
Question
Q: how do I analyze ELISA data? I have performed Chemerin level in plasma.
Verified Customer
Verified customer
Asked: 2016-02-11
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-02-11
Question
Which protein has EK1329 been raised against?
Verified Customer
Verified customer
Asked: 2014-10-23
Answer
For Human Chemerin/RARRES2 ELISA Kit PicoKine™ (EK1329), expression system for standard: E.coli; Immunogen sequence: V17-S163 , Uniprot Id: Q99969
Boster Scientific Support
Answered: 2014-10-23
