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Product Info Summary
| SKU: | A06142-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RSK3/RPS6KA2 Antibody Picoband®
SKU/Catalog Number
A06142-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RSK3/RPS6KA2 Antibody Picoband® catalog # A06142-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RSK3/RPS6KA2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06142-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human RSK3 recombinant protein (Position: H658-L733).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06142-1 is reactive to RPS6KA2 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90-100 kDa
Calculated molecular weight
83.239kDa
Background of RSK3
Ribosomal protein S6 kinase alpha-2 is an enzyme that in humans is encoded by the RPS6KA2 gene. It is mapped to 6q27. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains two non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternative splice variants, encoding different isoforms, have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06142-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunocytochemistry/Immunofluorescence, 2μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human placenta tissue, human Hela whole cell, human PC-3 whole cell, human A431 whole cell, human K562 whole cell, human PANC-1 whole cell, rat testis tissue, mouse testis tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RSK3 using anti-RSK3 antibody (A06142-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human Hela whole cell lysate,
Lane 3: human PC-3 whole cell lysate,
Lane 4: human A431 whole cell lysate,
Lane 5: human K562 whole cell lysate,
Lane 6: human PANC-1 whole cell lysate,
Lane 7: rat testis tissue lysates,
Lane 8: mouse testis tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSK3 antigen affinity purified polyclonal antibody (Catalog # A06142-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSK3 at approximately 90-100KD. The expected band size for RSK3 is at 83KD.
Protein Target Info & Infographic
Gene/Protein Information For RPS6KA2 (Source: Uniprot.org, NCBI)
Gene Name
RPS6KA2
Full Name
Ribosomal protein S6 kinase alpha-2
Weight
83.239kDa
Superfamily
protein kinase superfamily
Alternative Names
Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2; p90-RSK 2; p90RSK2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; Ribosomal S6 kinase 3; RSK-3; pp90RSK3; RPS6KA2; MAPKAPK1C; RSK3 RPS6KA2 HU-2, MAPKAPK1C, RSK, RSK3, S6K-alpha, S6K-alpha2, p90-RSK3, p90RSK2, pp90RSK3 ribosomal protein S6 kinase A2 ribosomal protein S6 kinase alpha-2|MAP kinase-activated protein kinase 1c|MAPK-activated protein kinase 1c|mitogen-activated protein kinase-activated protein kinase 1C|ribosomal S6 kinase 3|ribosomal protein S6 kinase, 90kDa, polypeptide 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPS6KA2, check out the RPS6KA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPS6KA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RSK3/RPS6KA2 Antibody Picoband® (A06142-1)
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1 Customer Q&As for Anti-RSK3/RPS6KA2 Antibody Picoband®
Question
We are currently using anti-RSK3/RPS6KA2 antibody A06142-1 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-03-20
Answer
The anti-RSK3/RPS6KA2 antibody (A06142-1) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-03-20
