Product Info Summary
SKU: | M03266 |
---|---|
Size: | 100ug |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Mesothelin MSLN Monoclonal Antibody
View all Mesothelin Antibodies
SKU/Catalog Number
M03266
Size
100ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Mesothelin MSLN Monoclonal Antibody (Catalog # M03266). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Mesothelin MSLN Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03266)
Host
Mouse
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Monoclonal
Clone Number
Clone: MN-1 IgG2a
Isotype
IgG2a
Immunogen
This antibody was produced in mesothelin-deficient mice by immunizations with plasmid cDNA encoding human MSLN full length protein followed by a single boost of a recombinant human mesothelin-Fc fusion protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M03266 is reactive to MSLN in Human
Reconstitution
Calculated molecular weight
68986 MW
Background of Mesothelin
Anti Mesothelin Antibody recognizes Mesothelin that is a glycosyl-phosphatidylinositol–anchored glycoprotein present on the cell surface of various human solid tumors. The mesothelin (MSLN) gene encodes a 71-kDa precursor protein that is processed to a 40-kDa glycosylphosphatidylinositol–anchored protein that composes the mature portion and an NH2 terminal 31-kDa fragment called megakaryocyte-potentiating factor that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues, such as mesothelium, but aberrantly over expressed in mesotheliomas, ovarian, and pancreatic cancers. The biological functions of mesothelin remain elusive. A recent study showed that mesothelin binds to MUC16/CA125, and that this interaction mediates cell adhesion, suggesting that there may be an important role for MUC16/CA125 and mesothelin in the metastatic spread of ovarian cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03266 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
Flow Cytometry: 1:800
IHC: 1:100
WB: 1:1,000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry using mouse anti-mesothelin monoclonal antibody (Catalog # M03266) to detect mesothelin in PEFF human tissue sections treated by antigen retrieval methods. Anti-mesothelin primary antibodies were used to label these sections as follows: C, MAb MB; and D, MAb MN. Reprinted with permission from Clin.Cancer Res. 11(16):5840-6.
Click image to see more details
Western blotting using mouse anti-mesothelin monoclonal antibody (Catalog # M03266) at 1
Protein Target Info & Infographic
Gene/Protein Information For MSLN (Source: Uniprot.org, NCBI)
Gene Name
MSLN
Full Name
Mesothelin
Weight
68986 MW
Superfamily
mesothelin family
Alternative Names
Mesothelin, Mesothelian, MN, MB, Pre-pro-megakaryocyte-potentiating factor, CAK1 antigen MSLN MPF, SMRP mesothelin mesothelin|CAK1 |megakaryocyte potentiating factor|pre-pro-megakaryocyte-potentiating factor|soluble MPF mesothelin related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MSLN, check out the MSLN Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MSLN: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Mesothelin MSLN Monoclonal Antibody (M03266)
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No publications found for M03266
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Customer Q&As
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15 Customer Q&As for Anti-Mesothelin MSLN Monoclonal Antibody
Question
We ordered your anti-Mesothelin Monoclonal antibody for WB on pancreatic tumor in the past. I am using human, and We are going to use the antibody for IHC next. My question regards examining pancreatic tumor as well as pancreatic cancer in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-04-14
Answer
I viewed the website and datasheets of our anti-Mesothelin Monoclonal antibody and it seems that M03266 has been tested on human in both WB and IHC. Thus M03266 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-14
Question
We are currently using anti-Mesothelin Monoclonal antibody M03266 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
The anti-Mesothelin Monoclonal antibody (M03266) has not been tested for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-20
Question
Would anti-Mesothelin Monoclonal antibody M03266 work for IHC with tear?
J. Rodriguez
Verified customer
Asked: 2019-09-13
Answer
According to the expression profile of tear, MSLN is highly expressed in tear. So, it is likely that anti-Mesothelin Monoclonal antibody M03266 will work for IHC with tear.
Boster Scientific Support
Answered: 2019-09-13
Question
I have attached the WB image, lot number and protocol we used for tear using anti-Mesothelin Monoclonal antibody M03266. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-02
Question
Is a blocking peptide available for product anti-Mesothelin Monoclonal antibody (M03266)?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
We do provide the blocking peptide for product anti-Mesothelin Monoclonal antibody (M03266). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-08
Question
you antibody to test anti-Mesothelin Monoclonal antibody M03266 on human tear for research purposes, then I may be interested in using anti-Mesothelin Monoclonal antibody M03266 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-10-29
Answer
The products we sell, including anti-Mesothelin Monoclonal antibody M03266, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-10-29
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tear using anti-Mesothelin Monoclonal antibody M03266. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-11
Question
Would M03266 anti-Mesothelin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-05-04
Answer
You can see on the product datasheet, M03266 anti-Mesothelin Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-05-04
Question
We were content with the WB result of your anti-Mesothelin Monoclonal antibody. However we have seen positive staining in pancreatic cancer cell membrane using this antibody. Is that expected? Could you tell me where is MSLN supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-03-16
Answer
From what I have seen in literature, pancreatic cancer does express MSLN. Generally MSLN expresses in cell membrane. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2018-03-16
Question
My question regarding product M03266, anti-Mesothelin Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
J. Baker
Verified customer
Asked: 2018-01-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03266 anti-Mesothelin Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-09
Question
I see that the anti-Mesothelin Monoclonal antibody M03266 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-12-28
Question
Do you have a BSA free version of anti-Mesothelin Monoclonal antibody M03266 available?
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Mesothelin Monoclonal antibody M03266 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-17
Question
I was wanting to use using your anti-Mesothelin Monoclonal antibody for cell-matrix adhesion studies. Has this antibody been tested with western blotting on peff human tissue? We would like to see some validation images before ordering.
J. Singh
Verified customer
Asked: 2017-05-03
Answer
Thanks for your inquiry. This M03266 anti-Mesothelin Monoclonal antibody is tested on peff human tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-05-03
Question
We have seen staining in human pancreatic tumor. Do you have any suggestions? Is anti-Mesothelin Monoclonal antibody supposed to stain pancreatic tumor positively?
B. Parker
Verified customer
Asked: 2016-09-08
Answer
From what I have seen in literature pancreatic tumor does express MSLN. From what I have seen in Uniprot.org, MSLN is expressed in right uterine tube, pancreatic cancer, carcinoma, lung spleen, pancreas placenta, pancreatic tumor, tear, among other tissues. Regarding which tissues have MSLN expression, here are a few articles citing expression in various tissues:
Carcinoma, Pubmed ID: 8552591
Lung, and Spleen, Pubmed ID: 15897581
Pancreas, and Placenta, Pubmed ID: 15489334
Pancreatic cancer, Pubmed ID: 7665620
Pancreatic tumor, Pubmed ID: 8288629
Tear, Pubmed ID: 25946035
Boster Scientific Support
Answered: 2016-09-08
Question
I was wanting to use your anti-Mesothelin Monoclonal antibody for IHC for human tear on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human tear identification?
N. Mitchell
Verified customer
Asked: 2013-09-03
Answer
It shows on the product datasheet, M03266 anti-Mesothelin Monoclonal antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tear in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-09-03