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Product Info Summary
| SKU: | EK1713 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®
View all Serpin F2/alpha 2-Antiplasmin ELISA kits
SKU/Catalog Number
EK1713
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Serpinf2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1713)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: N40-K491
Sensitivity
<10 pg/ml
Assay Range
1.56 ng/ml - 100 ng/ml
Standard Dilution Instructions
See datasheet of EK1713 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1713 is reactive to Serpinf2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK1713 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Serpin F2/alpha 2-Antiplasmin
Alpha-2-antiplasmin is a serine protease inhibitor (serpin) responsible for inactivating plasmin. Plasmin is an important enzyme that participates in fibrinolysis and degradation of various other proteins. This protein is encoded by the SERPINF2 gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1713-CAP | Anti-Mouse Serpinf2 Pre-coated 96-well strip microplate | 1 |
| EK1713-ST | Mouse Serpinf2 Standard | 2 vials, 100 ng/tube |
| EK1713-DA | Mouse Serpinf2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 1.56 | 3.13 | 6.25 | 12.5 | 25 | 50 | 100 |
| O.D. | 0.066 | 0.122 | 0.175 | 0.276 | 0.520 | 0.952 | 1.408 | 2.380 |
Data Example Images
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Mouse SerpinF2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Serpin F2/alpha 2-Antiplasmin, Dilution ratio of 1:1000, concentration in serum and plasma is 7.5-80 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 2710 | 13864 | 42376 | 2900 | 14200 | 45464 |
| Standard deviation | 113.82 | 804.1 | 2712.06 | 130.5 | 965.6 | 3273.4 |
| CV (%) | 4.2% | 5.8% | 6.4% | 4.5% | 6.8% | 7.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Serpin F2/alpha 2-Antiplasmin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 2710 | 2230 | 2706 | 2277 | 2480 | 227.86 | 9.1% |
| Sample . 2 | 13864 | 12175 | 13380 | 12105 | 12881 | 760.90 | 5.9%% |
| Sample 3 | 42376 | 42947 | 47567 | 46317 | 44801 | 2194.70 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For Serpinf2 (Source: Uniprot.Org, NCBI)
Gene Name
Serpinf2
Full Name
Alpha-2-antiplasmin
Weight
54.566kDa
Superfamily
serpin family
Alternative Names
Alpha-2-antiplasmin; Alpha-2-AP; Alpha-2-plasmin inhibitor; Alpha-2-PI; Serpin F2; Serpinf2; Pli SERPINF2 A2AP, AAP, ALPHA-2-PI, API, PLI, alpha2AP serpin family F member 2 alpha-2-antiplasmin|alpha-2-AP|alpha-2-plasmin inhibitor|plasmin inhibitor|serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2|serpin F2|serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Serpinf2, check out the Serpinf2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Serpinf2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine® (EK1713)
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Customer Q&As
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6 Customer Q&As for Mouse Serpin F2/alpha 2-Antiplasmin/SERPINF2 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
D. Chakrabarti
Verified customer
Asked: 2019-07-10
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-07-10
Question
Q: if the enzyme conjugated Serpin F2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin F2 antigen?
J. Phillips
Verified customer
Asked: 2018-06-24
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-06-24
Question
Q: What is the optimal O.D. value for Serpin F2 ELISA kit? I used your Serpin F2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Serpin F2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin F2 ELISA kit to have sensitivity of 10pg/ml , that means the minimum amount of Serpin F2 that can be declared/interpreted as positive by the above standard is 10pg/ml .
Boster Scientific Support
Answered: 2018-05-11
Question
Q: how to analyze ELISA data? I have performed Serpin F2 level in plasma .
Verified Customer
Verified customer
Asked: 2018-05-01
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-05-01
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Serpin F2 using the EK1713 Mouse Serpin F2 Picokine® ELISA Kit?
Z. Li
Verified customer
Asked: 2017-05-09
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2017-05-09
Question
Q: how to thaw whole blood sample for Serpin F2 ELISA after freezing?
R. Patel
Verified customer
Asked: 2017-02-15
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin F2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-02-15
