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Product Info Summary
| SKU: | EK0926 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CD244/2B4 ELISA Kit PicoKine®
View all 2B4/CD244/SLAMF4 ELISA kits
SKU/Catalog Number
EK0926
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD244/2B4 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD244 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD244/2B4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0926)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: C22-R221
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0926 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0926 is reactive to CD244 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0926 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of 2B4/CD244/SLAMF4
CD244 (Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Members of the CD2 family adhere to each other instead,the cell surface glycoprotein 2B4 is related to CD2 and is implicated in the regulation of NK- and T-cell function. The 2B4 gene was mapped to 1q22 on gene map, where SLAM, CD48, CD84 and LY9 are also located. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, It was found that CD48 binds to 2B4. BIAcore surface plasmon resonance analysis showed that the affinity of CD48 for 2B4 is approximately 10-fold higher than that shown for CD48 and CD2.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0926-CAP | Anti-Human CD244 Pre-coated 96-well strip microplate | 1 |
| EK0926-ST | Human CD244 Standard | 2 vials, 10 ng/tube |
| EK0926-DA | Human CD244 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.025 | 0.199 | 0.292 | 0.464 | 0.934 | 1.510 | 2.026 | 2.547 |
Data Example Images
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Human CD244/2B4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect 2B4/CD244/SLAMF4, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD244/2B4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 28 | 81 | 453 | 30 | 112 | 469 |
| Standard deviation | 1.34 | 4.62 | 20.84 | 1.53 | 6.61 | 30.02 |
| CV (%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of 2B4/CD244/SLAMF4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 28 | 27 | 30 | 28 | 28 | 1.08 | 3.8% |
| Sample 2 | 81 | 79 | 81 | 73 | 78 | 3.27 | 4.1% |
| Sample 3 | 453 | 479 | 435 | 456 | 455 | 15.64 | 3.4% |
Protein Target Info & Infographic
Gene/Protein Information For CD244 (Source: Uniprot.Org, NCBI)
Gene Name
CD244
Full Name
Natural killer cell receptor 2B4
Weight
41616 MW
Alternative Names
Natural killer cell receptor 2B4;NK cell activation-inducing ligand;NAIL;NK cell type I receptor protein 2B4;NKR2B4;h2B4;SLAM family member 4;SLAMF4;Signaling lymphocytic activation molecule 4;CD244;CD244;2B4; CD244 2B4, NAIL, NKR2B4, Nmrk, SLAMF4 CD244 molecule natural killer cell receptor 2B4|CD244 molecule, natural killer cell receptor 2B4|NK cell activation inducing ligand NAIL|NK cell activation-inducing ligand|NK cell type I receptor protein 2B4|SLAM family member 4|h2B4|signaling lymphocytic activation molecule 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD244, check out the CD244 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD244: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD244/2B4 ELISA Kit PicoKine® (EK0926)
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No publications found for EK0926
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Human CD244/2B4 ELISA Kit PicoKine®
Question
Q: Can CD244 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
W. Clark
Verified customer
Asked: 2020-10-05
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-10-05
Question
Q: how to thaw whole blood sample for CD244 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD244 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-03-16
Question
Are the standards in EK0926 natural material or recombinant? And if recombinant, what kind of cell or living material is used for expression?
Verified customer
Asked: 2019-12-02
Answer
For the Human CD244/2B4 ELISA Kit PicoKine™ (EK0926), the standard is recombinant and the expression system is NSO.
Boster Scientific Support
Answered: 2019-12-03
Question
Do the standards in EK0926 include additive agent such as antiseptic or preservative? If included, what is the composition of said additive agent?
Verified customer
Asked: 2019-12-02
Answer
The standard for the Human CD244/2B4 ELISA Kit PicoKine™ (EK0926) contains sodium azide (0.02%).
Boster Scientific Support
Answered: 2019-12-03
Question
What is the material name and concentration of Stop Solution for EK0926?
Verified customer
Asked: 2019-12-02
Answer
The concentration of Stop Solution for the Human CD244/2B4 ELISA Kit PicoKine™ (EK0926) is 2% Sulphuric acid.
Boster Scientific Support
Answered: 2019-12-03
Question
Does EK0926 include hazardous substance such as azide, thimerosal, 2-ME, etc?
Verified customer
Asked: 2019-12-02
Answer
The Human CD244/2B4 ELISA Kit PicoKine™ (EK0926) contains sodium azide (0.02%), sulphuric acid (2%) and proclin(0.2%).
Boster Scientific Support
Answered: 2019-12-03
Question
If the standards in EK0926 are recombinant, was a virus vector used in the recombination and/or expression process?
Verified customer
Asked: 2019-12-02
Answer
No, There wasn't any virus vector used in the recombination and/or expression process of the Human CD244/2B4 ELISA Kit PicoKine® (EK0926).
Boster Scientific Support
Answered: 2019-12-03
Question
Q: if the enzyme conjugated CD244 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD244 antigen?
Verified Customer
Verified customer
Asked: 2019-02-02
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-02-02
Question
Q: can I use citrate plasma as samples in Human CD244 Picokine® ELISA Kit (Catalog # EK0926)?
M. Baker
Verified customer
Asked: 2018-12-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CD244 causing degradation of its protein structure. CD244 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the CD244 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-12-04
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
H. Kaur
Verified customer
Asked: 2018-07-23
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-07-23
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
P. Dhawan
Verified customer
Asked: 2018-07-01
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-07-01
Question
Q: What is the optimal O.D. value for CD244 ELISA kit? I used your CD244 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD244 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-05-15
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD244 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD244 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-05-15
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. King
Verified customer
Asked: 2018-04-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-08
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CD244 in Human cell lysates? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-12-08
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD244 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-12-08
Question
Q: how to analyze ELISA data? I measured CD244 level in cell lysates.
C. Davis
Verified customer
Asked: 2017-08-31
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-08-31
